RT Journal Article SR Electronic(1) A1 Moon, Hee-Won A1 Lee, Sun Hwa A1 Chung, Hae-Sun A1 Lee, Miae A1 Lee, KyungwonYR 2013 T1 Performance of the Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry system for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories JF Journal of Medical Microbiology, VO 62 IS 9 SP 1301 OP 1306 DO https://doi.org/10.1099/jmm.0.062950-0 PB Microbiology Society, SN 1473-5644, AB We evaluated the performance of the Vitek MS for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. With a total of 424 well-characterized isolates, the results of the Vitek MS were compared to those of conventional methods and 16S rRNA gene sequencing. The Vitek MS correctly identified 97.9 % of the isolates tested to species level. The Vitek MS correctly identified the species of 97.2 % of the staphylococci (95.9 % of coagulase-negative staphylococci), 97.8 % of the streptococci, and 100 % of the enterococci. For the identification of Gram-positive cocci isolates, the overall concordance rate between conventional identification and the Vitek MS was 94.5 %. The Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) system can be a reliable and rapid method for the identification of most relevant Gram-positive cocci. In addition, expanding the database of the Vitek MS, especially for coagulase-negative staphylococci, is needed to enhance the performance of the Vitek MS., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.062950-0