1887

Abstract

A total of 69 strains of from patients in the Taizhou Municipal Hospital, China, were isolated, and 11 strains were identified that were resistant to bacitracin, chloramphenicol, tetracycline and erythromycin. These strains were PCR positive for at least two out of three genes, , and , by gene mapping with conventional PCR detection. Conjugation experiments demonstrated that these genes existed in plasmids that conferred resistance. Novel and variants were isolated from strains EC2163 and EC2347, which were obtained from the sputum of intensive care unit patients. Genetic mapping showed that the genes were located on 8200 kb plasmid regions flanked by RI restriction sites. Three distinct genetic structures were identified among the 11 PCR-positive strains of , and two contained the novel and variants. The putative amino acid differences in the and gene variants were characterized. These results provide evidence for novel variants of and , and suggest that multiple drug resistance in hospital strains of depends on the synergistic function of , and within three distinct genetic structures in conjugative plasmids.

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2013-11-01
2019-10-16
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