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Abstract

A total of 1878 non-duplicate clinical isolates (comprising 1711 urinary isolates and 167 blood-culture isolates), which were collected from multiple centres in Hong Kong during 1996–2008, were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin () resistance genes. Eighteen of the 1878 clinical isolates were fosfomycin resistant, of which six were positive and two were positive for another variant (designated ). No isolates had the gene. The clones of the eight isolates were diverse: sequence type (ST) 95 ( = 2), ST118 ( = 1), ST131 ( = 1), ST617 ( = 1), ST648 ( = 1), ST1488 ( = 1) and ST2847 ( = 1). In the isolates, and genes were co-harboured on conjugative plasmids with F2:A−:B− ( = 2), N ( = 1), F–:A−:B1 and N ( = 1) and untypable ( = 2) replicons. Both -carrying plasmids belonged to replicon N. RFLP analysis showed that the two F2:A−:B− plasmids carrying and genes shared the same pattern. Complete sequencing of one of the two F2:A−:B− plasmids, pFOS-HK151325 (69 768 bp) demonstrated it to be >99 % identical to the previously sequenced plasmid pHK23a originating from a pig isolate in the same region. This study demonstrated the dissemination of genes in diverse clones on multiple -carrying plasmid types, of which F2:A−:B− plasmids closely related to pHK23a were shared by isolates from human and animal sources.

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2013-11-01
2024-03-28
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