1887

Abstract

A total of 1878 non-duplicate clinical isolates (comprising 1711 urinary isolates and 167 blood-culture isolates), which were collected from multiple centres in Hong Kong during 1996–2008, were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin () resistance genes. Eighteen of the 1878 clinical isolates were fosfomycin resistant, of which six were positive and two were positive for another variant (designated ). No isolates had the gene. The clones of the eight isolates were diverse: sequence type (ST) 95 ( = 2), ST118 ( = 1), ST131 ( = 1), ST617 ( = 1), ST648 ( = 1), ST1488 ( = 1) and ST2847 ( = 1). In the isolates, and genes were co-harboured on conjugative plasmids with F2:A−:B− ( = 2), N ( = 1), F–:A−:B1 and N ( = 1) and untypable ( = 2) replicons. Both -carrying plasmids belonged to replicon N. RFLP analysis showed that the two F2:A−:B− plasmids carrying and genes shared the same pattern. Complete sequencing of one of the two F2:A−:B− plasmids, pFOS-HK151325 (69 768 bp) demonstrated it to be >99 % identical to the previously sequenced plasmid pHK23a originating from a pig isolate in the same region. This study demonstrated the dissemination of genes in diverse clones on multiple -carrying plasmid types, of which F2:A−:B− plasmids closely related to pHK23a were shared by isolates from human and animal sources.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.062653-0
2013-11-01
2019-09-22
Loading full text...

Full text loading...

/deliver/fulltext/jmm/62/11/1707.html?itemId=/content/journal/jmm/10.1099/jmm.0.062653-0&mimeType=html&fmt=ahah

References

  1. Aziz R. K., Bartels D., Best A. A., DeJongh M., Disz T., Edwards R. A., Formsma K., Gerdes S., Glass E. M.. & other authors ( 2008;). The RAST Server: rapid annotations using subsystems technology. . BMC Genomics 9:, 75. [CrossRef][PubMed]
    [Google Scholar]
  2. CLSI ( 2012;). Performance Standards for Antimicrobial Susceptibility Testing; 22nd Informational Supplement M100-S22. . Wayne, PA, USA:: CLSI;.
  3. Doumith M., Day M. J., Hope R., Wain J., Woodford N.. ( 2012;). Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups. . J Clin Microbiol 50:, 3108–3110. [CrossRef][PubMed]
    [Google Scholar]
  4. Gupta K., Hooton T. M., Naber K. G., Wullt B., Colgan R., Miller L. G., Moran G. J., Nicolle L. E., Raz R.. & other authors ( 2011;). International clinical practice guidelines for the treatment of acute uncomplicated cystitis and pyelonephritis in women: a 2010 update by the Infectious Diseases Society of America and the European Society for Microbiology and Infectious Diseases. . Clin Infect Dis 52:, e103–e120. [CrossRef][PubMed]
    [Google Scholar]
  5. Ho P. L., Wong R. C., Yip K. S., Loke S. L., Leung M. S., Mak G. C., Chow F. K., Tsang K. W., Que T. L..COMBAT study group ( 2007;). Antimicrobial resistance in Escherichia coli outpatient urinary isolates from women: emerging multidrug resistance phenotypes. . Diagn Microbiol Infect Dis 59:, 439–445. [CrossRef][PubMed]
    [Google Scholar]
  6. Ho P. L., Yip K. S., Chow K. H., Lo J. Y., Que T. L., Yuen K. Y.. ( 2010;). Antimicrobial resistance among uropathogens that cause acute uncomplicated cystitis in women in Hong Kong: a prospective multicenter study in 2006 to 2008. . Diagn Microbiol Infect Dis 66:, 87–93. [CrossRef][PubMed]
    [Google Scholar]
  7. Ho P. L., Lo W. U., Wong R. C., Yeung M. K., Chow K. H., Que T. L., Tong A. H., Bao J. Y., Lok S., Wong S. S.. ( 2011a;). Complete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong. . J Antimicrob Chemother 66:, 752–756. [CrossRef][PubMed]
    [Google Scholar]
  8. Ho P. L., Lo W. U., Yeung M. K., Lin C. H., Chow K. H., Ang I., Tong A. H., Bao J. Y., Lok S., Lo J. Y.. ( 2011b;). Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong. . PLoS ONE 6:, e17989. [CrossRef][PubMed]
    [Google Scholar]
  9. Ho P. L., Lo W. U., Yeung M. K., Li Z., Chan J., Chow K. H., Yam W. C., Tong A. H., Bao J. Y.. & other authors ( 2012a;). Dissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources. . Vet Microbiol 158:, 172–179. [CrossRef][PubMed]
    [Google Scholar]
  10. Ho P. L., Yeung M. K., Lo W. U., Tse H., Li Z., Lai E. L., Chow K. H., To K. K., Yam W. C.. ( 2012b;). Predominance of pHK01-like incompatibility group FII plasmids encoding CTX-M-14 among extended-spectrum β-lactamase-producing Escherichia coli in Hong Kong, 1996-2008. . Diagn Microbiol Infect Dis 73:, 182–186. [CrossRef][PubMed]
    [Google Scholar]
  11. Ho P. L., Chan J., Lo W. U., Law P. Y., Chow K. H.. ( 2013a;). Plasmid-mediated fosfomycin resistance in Escherichia coli isolated from pig. . Vet Microbiol 162:, 964–967. [CrossRef][PubMed]
    [Google Scholar]
  12. Ho P. L., Chan J., Lo W. U., Law P. Y., Li Z., Lai E. L., Chow K. H.. ( 2013b;). Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals. . J Appl Microbiol 114:, 695–702. [CrossRef][PubMed]
    [Google Scholar]
  13. Hou J., Huang X., Deng Y., He L., Yang T., Zeng Z., Chen Z., Liu J. H.. ( 2012;). Dissemination of the fosfomycin resistance gene fosA3 with CTX-M β-lactamase genes and rmtB carried on IncFII plasmids among Escherichia coli isolates from pets in China. . Antimicrob Agents Chemother 56:, 2135–2138. [CrossRef][PubMed]
    [Google Scholar]
  14. Johnson T. J., Nolan L. K.. ( 2009;). Plasmid replicon typing. . Methods Mol Biol 551:, 27–35. [CrossRef][PubMed]
    [Google Scholar]
  15. Johnson T. J., Bielak E. M., Fortini D., Hansen L. H., Hasman H., Debroy C., Nolan L. K., Carattoli A.. ( 2012;). Expansion of the IncX plasmid family for improved identification and typing of novel plasmids in drug-resistant Enterobacteriaceae. . Plasmid 68:, 43–50. [CrossRef][PubMed]
    [Google Scholar]
  16. Lee S. Y., Park Y. J., Yu J. K., Jung S., Kim Y., Jeong S. H., Arakawa Y.. ( 2012;). Prevalence of acquired fosfomycin resistance among extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae clinical isolates in Korea and IS26-composite transposon surrounding fosA3. . J Antimicrob Chemother 67:, 2843–2847. [CrossRef][PubMed]
    [Google Scholar]
  17. Lo W. U., Ho P. L., Chow K. H., Lai E. L., Yeung F., Chiu S. S.. ( 2010;). Fecal carriage of CTXM type extended-spectrum β-lactamase-producing organisms by children and their household contacts. . J Infect 60:, 286–292. [CrossRef][PubMed]
    [Google Scholar]
  18. Lo W. U., Cheung Y. Y., Lai E., Lung D., Que T. L., Ho P. L.. ( 2013;). Complete sequence of an IncN plasmid, pIMP-HZ1, carrying blaIMP-4 in a Klebsiella pneumoniae strain associated with medical travel to China. . Antimicrob Agents Chemother 57:, 1561–1562. [CrossRef][PubMed]
    [Google Scholar]
  19. Mendoza C., Garcia J. M., Llaneza J., Mendez F. J., Hardisson C., Ortiz J. M.. ( 1980;). Plasmid-determined resistance to fosfomycin in Serratia marcescens. . Antimicrob Agents Chemother 18:, 215–219. [CrossRef][PubMed]
    [Google Scholar]
  20. Partridge S. R., Paulsen I. T., Iredell J. R.. ( 2012;). pJIE137 carrying blaCTX-M-62 is closely related to p271A carrying blaNDM-1. . Antimicrob Agents Chemother 56:, 2166–2168. [CrossRef][PubMed]
    [Google Scholar]
  21. Sekizuka T., Matsui M., Yamane K., Takeuchi F., Ohnishi M., Hishinuma A., Arakawa Y., Kuroda M.. ( 2011;). Complete sequencing of the blaNDM-1-positive IncA/C plasmid from Escherichia coli ST38 isolate suggests a possible origin from plant pathogens. . PLoS ONE 6:, e25334. [CrossRef][PubMed]
    [Google Scholar]
  22. Shen P., Jiang Y., Zhou Z., Zhang J., Yu Y., Li L.. ( 2008;). Complete nucleotide sequence of pKP96, a 67 850 bp multiresistance plasmid encoding qnrA1, aac(6′)-Ib-cr and blaCTX-M-24 from Klebsiella pneumoniae. . J Antimicrob Chemother 62:, 1252–1256. [CrossRef][PubMed]
    [Google Scholar]
  23. Takahata S., Ida T., Hiraishi T., Sakakibara S., Maebashi K., Terada S., Muratani T., Matsumoto T., Nakahama C., Tomono K.. ( 2010;). Molecular mechanisms of fosfomycin resistance in clinical isolates of Escherichia coli. . Int J Antimicrob Agents 35:, 333–337. [CrossRef][PubMed]
    [Google Scholar]
  24. Villa L., García-Fernández A., Fortini D., Carattoli A.. ( 2010;). Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants. . J Antimicrob Chemother 65:, 2518–2529. [CrossRef][PubMed]
    [Google Scholar]
  25. Wachino J., Yamane K., Suzuki S., Kimura K., Arakawa Y.. ( 2010;). Prevalence of fosfomycin resistance among CTX-M-producing Escherichia coli clinical isolates in Japan and identification of novel plasmid-mediated fosfomycin-modifying enzymes. . Antimicrob Agents Chemother 54:, 3061–3064. [CrossRef][PubMed]
    [Google Scholar]
  26. Zhou Y., Yu H., Guo Q., Xu X., Ye X., Wu S., Guo Y., Wang M.. ( 2010;). Distribution of 16S rRNA methylases among different species of Gram-negative bacilli with high-level resistance to aminoglycosides. . Eur J Clin Microbiol Infect Dis 29:, 1349–1353. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.062653-0
Loading
/content/journal/jmm/10.1099/jmm.0.062653-0
Loading

Data & Media loading...

Supplementary material 

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error