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Volume 62, Issue 11

Enhancement of the immune response against infection of mice by heat-killed multispecies combinations of lactic acid bacteria Free

Abstract

Heat-killed lactic acid bacteria (LAB) has advantages over live LAB in that it has a long shelf‐life and is therefore easy to store and transport. From four LAB strains selected by immunomodulatory activity and adherent properties, we prepared the heat-killed multispecies combination of LAB (MLAB) and the cell walls from MLAB under two conditions (100 °C for 30 min and 121 °C for 15 min). Different effects on the adherent properties of these four LAB strains were observed, depending on the heating conditions. With mouse macrophage cells, the two heat-killed MLABs (HMLABs) showed significantly higher induction activities on the production of interleukin 12 (IL-12) than their individual strains did. Heat-killed MLABs and cell‐wall preparations were able to reduce the invasion of Caco-2 and mouse macrophage cells. Feeding mice with HMLAB could inhibit the invasion of mice significantly. For these mice, the expression level of pro-inflammatory cytokines, such as TNF-α and IL-6, in mouse serum was reduced while that of the anti-inflammatory cytokine, i.e. IL-10, was enhanced. The HMLABs developed in this study showed higher protective effect against invasion either of Caco-2 cells or of mice, relative to the heat-killed lactobacilli, which consisted of strains selected at random. In conclusion, the HMLABs were potentially useful for the protection of mice against infection and the induced inflammation.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-11-01
2024-04-16
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Enhancement of the immune response against Salmonella infection of mice by heat-killed multispecies combinations of lactic acid bacteria
J. Med. Microbiol. 62, 1657 (2013); https://doi.org/10.1099/jmm.0.061010-0
/content/journal/jmm/10.1099/jmm.0.061010-0
/content/journal/jmm/10.1099/jmm.0.061010-0
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