1887

Journal of Medical Microbiology logo

Volume 62, Issue 8

High prevalence of VIM-4 and NDM-1 metallo-β-lactamase among carbapenem-resistant Free

Abstract

The purpose of this study was to identify the mechanisms leading to carbapenem resistance among multidrug-resistant isolates recovered from hospitalized patients with nosocomial infections in Mubarak Al Kabeer Hospital, Kuwait. Fourteen carbapenem-resistant isolates were obtained from inpatients in different wards and intensive care units between April 2009 and February 2011. Antibiotic susceptibilities were determined using the E-test method. Genes encoding β-lactamases were characterized by specific PCR amplification, sequencing and conjugation assays. All isolates were identified as metallo-β-lactamase (MBL) producers using phenotypic and molecular methods. Eleven of the 14 isolates produced VIM-4 (six , three , one and one ). Three isolates produced the MBL NDM-1 and co-produced the plasmid-encoded AmpC CMY-4. The VIM-4-producing isolates co-produced extended-spectrum β-lactamases including CTX-M-15 and some SHV derivatives. The VIM-4 gene was not transferable by conjugation studies of six selected strains. We demonstrated here the emergence of VIM-4- and NDM-1-producing isolates in the largest teaching hospital in Kuwait.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.059915-0
2013-08-01
2023-09-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/62/8/1239.html?itemId=/content/journal/jmm/10.1099/jmm.0.059915-0&mimeType=html&fmt=ahah

References

  1. Al Sweih N., Al Hashem G., Jamal W., Rotimi V. O. 2010; National surveillance of antimicrobial susceptibility of CTX-M-positive and -negative clinical isolates of Escherichia coli from Kuwait government hospitals. J Chemother 22:254–258[PubMed] [CrossRef]
     [Google Scholar]
  2. Bratu S., Tolaney P., Karumudi U., Quale J., Mooty M., Nichani S., Landman D. 2005; Carbapenemase-producing Klebsiella pneumoniae in Brooklyn, NY: molecular epidemiology and in vitro activity of polymyxin B and other agents. J Antimicrob Chemother 56:128–132 [View Article][PubMed]
     [Google Scholar]
  3. Bush K. 1998; Metallo-β-lactamases: a class apart. Clin Infect Dis 27:Suppl 1S48–S53 [View Article][PubMed]
     [Google Scholar]
  4. CLSI 2012; Performance Standards for Antimicrobial Susceptibility Testing, 22nd Informational Supplement M100–S22. Wayne, PA: Clinical and Laboratory Standards Institute;
     [Google Scholar]
  5. Dortet L., Poirel L., Al Yaqoubi F., Nordmann P. 2012; NDM-1, OXA-48 and OXA-181 carbapenemase-producing Enterobacteriaceae in Sultanate of Oman. Clin Microbiol Infect 18:E144–E148 [View Article][PubMed]
     [Google Scholar]
  6. El-Herte R. I., Kanj S. S., Matar G. M., Araj G. F. 2012; The threat of carbapenem-resistant Enterobacteriaceae in Lebanon: an update on the regional and local epidemiology. J Infect Public Health 5:233–243 [View Article][PubMed]
     [Google Scholar]
  7. Fevre C., Passet V., Weill F.-X., Grimont P. A., Brisse S. 2005; Variants of the Klebsiella pneumoniae OKP chromosomal β-lactamase are divided into two main groups, OKP-A and OKP-B. Antimicrob Agents Chemother 49:5149–5152 [View Article][PubMed]
     [Google Scholar]
  8. Figueiredo S., Poirel L., Papa A., Koulourida V., Nordmann P. 2008; First identification of VIM-4 metallo-β-lactamase in Acinetobacter spp. Clin Microbiol Infect 14:289–290 [View Article][PubMed]
     [Google Scholar]
  9. Galani I., Souli M., Chryssouli Z., Orlandou K., Giamarellou H. 2005; Characterization of a new integron containing bla VIM-1 and aac(6′)-IIc in an Enterobacter cloacae clinical isolate from Greece. J Antimicrob Chemother 55:634–638 [View Article][PubMed]
     [Google Scholar]
  10. Jamal W., Rotimi V. O., Albert M. J., Khodakhast F., Udo E. E., Poirel L. 2012; Emergence of nosocomial New Delhi metallo-β-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae in patients admitted to a tertiary care hospital in Kuwait. Int J Antimicrob Agents 39:183–184 [View Article][PubMed]
     [Google Scholar]
  11. Kassis-Chikhani N., Decré D., Gautier V., Burghoffer B., Saliba F., Mathieu D., Samuel D., Castaing D., Petit J. C. other authors 2006; First outbreak of multidrug-resistant Klebsiella pneumoniae carrying bla VIM-1 and bla SHV-5 in a French university hospital. J Antimicrob Chemother 57:142–145 [View Article][PubMed]
     [Google Scholar]
  12. Ktari S., Arlet G., Mnif B., Gautier V., Mahjoubi F., Ben Jmeaa M., Bouaziz M., Hammami A. 2006; Emergence of multidrug-resistant Klebsiella pneumoniae isolates producing VIM-4 metallo-β-lactamase, CTX-M-15 extended-spectrum β-lactamase, and CMY-4 AmpC β-lactamase in a Tunisian university hospital. Antimicrob Agents Chemother 50:4198–4201 [View Article][PubMed]
     [Google Scholar]
  13. Kumarasamy K. K., Toleman M. A., Walsh T. R., Bagaria J., Butt F., Balakrishnan R., Chaudhary U., Doumith M., Giske C. G. other authors 2010; Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis 10:597–602 [View Article][PubMed]
     [Google Scholar]
  14. Lee K., Chong Y., Shin H. B., Kim Y. A., Yong D., Yum J. H. 2001; Modified Hodge and EDTA-disk synergy tests to screen metallo-β-lactamase-producing strains of Pseudomonas and Acinetobacter species. Clin Microbiol Infect 7:88–91 [View Article][PubMed]
     [Google Scholar]
  15. Lee K., Kim C. K., Yong D., Jeong S. H., Yum J. H., Seo Y. H., Docquier J.-D., Chong Y. 2010; Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli. J Microbiol Methods 83:149–152 [View Article][PubMed]
     [Google Scholar]
  16. Libisch B., Giske C. G., Kovács B., Tóth T. G., Füzi M. 2008; Identification of the first VIM metallo-β-lactamase-producing multiresistant Aeromonas hydrophila strain. J Clin Microbiol 46:1878–1880 [View Article][PubMed]
     [Google Scholar]
  17. Luzzaro F., Docquier J. D., Colinon C., Endimiani A., Lombardi G., Amicosante G., Rossolini G. M., Toniolo A. 2004; Emergence in Klebsiella pneumoniae and Enterobacter cloacae clinical isolates of the VIM-4 metallo-β-lactamase encoded by a conjugative plasmid. Antimicrob Agents Chemother 48:648–650 [View Article][PubMed]
     [Google Scholar]
  18. Marchaim D., Navon-Venezia S., Schwaber M. J., Carmeli Y. 2008; Isolation of imipenem-resistant Enterobacter species: emergence of KPC-2 carbapenemase, molecular characterization, epidemiology, and outcomes. Antimicrob Agents Chemother 52:1413–1418 [View Article][PubMed]
     [Google Scholar]
  19. Mataseje L. F., Bryce E., Roscoe D., Boyd D. A., Embree J., Gravel D., Katz K., Kibsey P., Kuhn M. other authors 2012; Carbapenem-resistant Gram-negative bacilli in Canada 2009–10: results from the Canadian Nosocomial Infection Surveillance Program (CNISP). J Antimicrob Chemother 67:1359–1367 [View Article][PubMed]
     [Google Scholar]
  20. Nordmann P., Naas T., Poirel L. 2011a; Global spread of carbapenemase-producing Enterobacteriaceae. . Emerg Infect Dis 17:1791–1798 [View Article][PubMed]
     [Google Scholar]
  21. Poirel L., Naas T., Nicolas D., Collet L., Bellais S., Cavallo J.-D., Nordmann P. 2000; Characterization of VIM-2, a carbapenem-hydrolyzing metallo-β-lactamase and its plasmid- and integron-borne gene from a Pseudomonas aeruginosa clinical isolate in France. Antimicrob Agents Chemother 44:891–897 [View Article][PubMed]
     [Google Scholar]
  22. Poirel L., Dortet L., Bernabeu S., Nordmann P. 2011a; Genetic features of bla NDM-1-positive Enterobacteriaceae. . Antimicrob Agents Chemother 55:5403–5407 [View Article][PubMed]
     [Google Scholar]
  23. Poirel L., Fortineau N., Nordmann P. 2011b; International transfer of NDM-1-producing Klebsiella pneumoniae from Iraq to France. Antimicrob Agents Chemother 55:1821–1822 [View Article][PubMed]
     [Google Scholar]
  24. Poirel L., Walsh T. R., Cuvillier V., Nordmann P. 2011c; Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis 70:119–123 [View Article][PubMed]
     [Google Scholar]
  25. Poirel L., Ozdamar M., Ocampo-Sosa A. A., Türkoglu S., Ozer U. G., Nordmann P. 2012; NDM-1-producing Klebsiella pneumoniae now in Turkey. Antimicrob Agents Chemother 56:2784–2785 [View Article][PubMed]
     [Google Scholar]
  26. Poirel L., Abdelaziz M. O., Bernabeu S., Nordmann P. 2013; Occurrence of OXA-48 and VIM-1 carbapenemase-producing Enterobacteriaceae in Egypt. Int J Antimicrob Agents 41:90–91 [View Article][PubMed]
     [Google Scholar]
  27. Pournaras S., Tsakris A., Maniati M., Tzouvelekis L. S., Maniatis A. N. 2002; Novel variant (bla VIM-4) of the metallo-β-lactamase gene bla VIM-1 in a clinical strain of Pseudomonas aeruginosa . Antimicrob Agents Chemother 46:4026–4028 [View Article][PubMed]
     [Google Scholar]
  28. Queenan A. M., Bush K. 2007; Carbapenemases: the versatile β-lactamases. Clin Microbiol Rev 20:440–458 [View Article][PubMed]
     [Google Scholar]
  29. Rotimi V. O., Jamal W., Pal T., Sovenned A., Albert M. J. 2008; Emergence of CTX-M-15 type extended-spectrum β-lactamase-producing Salmonella spp. in Kuwait and the United Arab Emirates. J Med Microbiol 57:881–886 [View Article][PubMed]
     [Google Scholar]
  30. Shevchenko O. V., Mudrak D. Y., Skleenova E. Y., Kozyreva V. K., Ilina E. N., Ikryannikova L. N., Alexandrova I. A., Sidorenko S. V., Edelstein M. V. 2012; First detection of VIM-4 metallo-β-lactamase-producing Escherichia coli in Russia. Clin Microbiol Infect 18:E214–E217 [View Article][PubMed]
     [Google Scholar]
  31. Sonnevend A., Ghazawi A., Yahfoufi N., Al-Baloushi A., Hashmey R., Mathew M., Tariq W. Z., Pál T. 2012; VIM-4 carbapenemase-producing Enterobacter cloacae in the United Arab Emirates. Clin Microbiol Infect 18:E494–E496[PubMed] [CrossRef]
     [Google Scholar]
  32. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. 1995; Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233–2239[PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.059915-0
Loading
High prevalence of VIM-4 and NDM-1 metallo-β-lactamase among carbapenem-resistant Enterobacteriaceae
J. Med. Microbiol. 62, 1239 (2013); https://doi.org/10.1099/jmm.0.059915-0
/content/journal/jmm/10.1099/jmm.0.059915-0
/content/journal/jmm/10.1099/jmm.0.059915-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error