1887

Abstract

Among 28 clinically relevant, carbapenem non-susceptible isolates collected in 2009–2011 in the United Arab Emirates three , two , one and one were identified to produce NDM-1 carbapenemase. Unexpectedly, with the exception of a strain, sequence type ST11, originally acquired in India and subsequently spread nosocomially in the UAE, the majority of the strains could not be directly linked to foreign travel. All isolates harboured the gene on plasmids of IncA/C, IncHI1b and IncX3 types, or were untypable. IncX3 type plasmids with a mass of 50 kb and with the same or highly similar restriction patterns, with regions flanking the gene identical to the IncX3 NDM plasmids described from China were present in three different species, , and . Our findings strongly support the assumptions that, beyond the Indian subcontinent, the Middle East is an important reservoir of NDM-producing organisms. Furthermore, we also provide evidence that IncX3 plasmids, recently implicated in the spread of in China, have been widely distributed and are important vehicles of the inter-species spread of the NDM-1 gene.

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2013-07-01
2019-11-20
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References

  1. Carattoli A., Miriagou V., Bertini A., Loli A., Colinon C., Villa L., Whichard J. M., Rossolini G. M.. ( 2006;). Replicon typing of plasmids encoding resistance to newer beta-lactams. . Emerg Infect Dis 12:, 1145–1148. [CrossRef][PubMed]
    [Google Scholar]
  2. Chen Y. T., Lin A. C., Siu L. K., Koh T. H.. ( 2012;). Sequence of closely related plasmids encoding bla(NDM-1) in two unrelated Klebsiella pneumoniae isolates in Singapore. . PLoS ONE 7:, e48737. [CrossRef][PubMed]
    [Google Scholar]
  3. CLSI ( 2012;). Performance Standards for Antimicrobial Susceptibility Testing, M100–S22. Wayne, PA:: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  4. Damjanova I., Tóth A., Pászti J., Hajbel-Vékony G., Jakab M., Berta J., Milch H., Füzi M.. ( 2008;). Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type β-lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005–the new ‘MRSAs’?. J Antimicrob Chemother 62:, 978–985. [CrossRef][PubMed]
    [Google Scholar]
  5. Denis C., Poirel L., Carricajo A., Grattard F., Fascia P., Verhoeven P., Gay P., Nuti C., Nordmann P. et al. ( 2012;). Nosocomial transmission of NDM-1-producing Escherichia coli within a non-endemic area in France. . Clin Microbiol Infect 18:, E128–E130. [CrossRef][PubMed]
    [Google Scholar]
  6. Diancourt L., Passet V., Verhoef J., Grimont P. A., Brisse S.. ( 2005;). Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. . J Clin Microbiol 43:, 4178–4182. [CrossRef][PubMed]
    [Google Scholar]
  7. Dortet L., Poirel L., Al Yaqoubi F., Nordmann P.. ( 2012;). NDM-1, OXA-48 and OXA-181 carbapenemase-producing Enterobacteriaceae in Sultanate of Oman. . Clin Microbiol Infect 18:, E144–E148. [CrossRef][PubMed]
    [Google Scholar]
  8. Gautom R. K.. ( 1997;). Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157 : H7 and other gram-negative organisms in 1 day. . J Clin Microbiol 35:, 2977–2980.[PubMed]
    [Google Scholar]
  9. Ghazawi A., Sonnevend A., Bonnin R. A., Poirel L., Nordmann P., Hashmey R., Rizvi T. A., B Hamadeh M., Pál T.. ( 2012;). NDM-2 carbapenemase-producing Acinetobacter baumannii in the United Arab Emirates. . Clin Microbiol Infect 18:, E34–E36. [CrossRef][PubMed]
    [Google Scholar]
  10. Giske C. G., Fröding I., Hasan C. M., Turlej-Rogacka A., Toleman M., Livermore D., Woodford N., Walsh T. R.. ( 2012;). Diverse sequence types of Klebsiella pneumoniae contribute to the dissemination of blaNDM-1 in India, Sweden, and the United Kingdom. . Antimicrob Agents Chemother 56:, 2735–2738. [CrossRef][PubMed]
    [Google Scholar]
  11. Ho P.-L., Li Z., Lo W.-U., Cheung Y.-Y., Lin C.-H., Sham P.-C., Cheng V. C.-C., Ng T.-K., Que T.-L., Chow K.-H.. ( 2012;). Identification and characterization of a novel incompatibility group X3 plasmid carrying blaNDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China. . Emerg Microbes Inf 1:, e39. [CrossRef]
    [Google Scholar]
  12. Jamal W., Rotimi V. O., Albert M. J., Khodakhast F., Udo E. E., Poirel L.. ( 2012;). Emergence of nosocomial New Delhi metallo-β-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae in patients admitted to a tertiary care hospital in Kuwait. . Int J Antimicrob Agents 39:, 183–184. [CrossRef][PubMed]
    [Google Scholar]
  13. Johnson T. J., Bielak E. M., Fortini D., Hansen L. H., Hasman H., Debroy C., Nolan L. K., Carattoli A.. ( 2012;). Expansion of the IncX plasmid family for improved identification and typing of novel plasmids in drug-resistant Enterobacteriaceae.. Plasmid 68:, 43–50. [CrossRef][PubMed]
    [Google Scholar]
  14. Kado C. I., Liu S. T.. ( 1981;). Rapid procedure for detection and isolation of large and small plasmids. . J Bacteriol 145:, 1365–1373.[PubMed]
    [Google Scholar]
  15. Lee M. Y., Ko K. S., Kang C. I., Chung D. R., Peck K. R., Song J. H.. ( 2011;). High prevalence of CTX-M-15-producing Klebsiella pneumoniae isolates in Asian countries: diverse clones and clonal dissemination. . Int J Antimicrob Agents 38:, 160–163. [CrossRef][PubMed]
    [Google Scholar]
  16. Nordmann P., Poirel L., Walsh T. R., Livermore D. M.. ( 2011;). The emerging NDM carbapenemases. . Trends Microbiol 19:, 588–595. [CrossRef][PubMed]
    [Google Scholar]
  17. Pereira P. S., de Araujo C. F., Seki L. M., Zahner V., Carvalho-Assef A. P., Asensi M. D.. ( 2013;). Update of the molecular epidemiology of KPC-2-producing Klebsiella pneumoniae in Brazil: spread of clonal complex 11 (ST11, ST437 and ST340). . J Antimicrob Chemother 68:, 312–316. [CrossRef][PubMed]
    [Google Scholar]
  18. Pérez-Pérez F. J., Hanson N. D.. ( 2002;). Detection of plasmid-mediated AmpC β-lactamase genes in clinical isolates by using multiplex PCR. . J Clin Microbiol 40:, 2153–2162. [CrossRef][PubMed]
    [Google Scholar]
  19. Poirel L., Fortineau N., Nordmann P.. ( 2011a;). International transfer of NDM-1-producing Klebsiella pneumoniae from Iraq to France. . Antimicrob Agents Chemother 55:, 1821–1822. [CrossRef][PubMed]
    [Google Scholar]
  20. Poirel L., Dortet L., Bernabeu S., Nordmann P.. ( 2011b;). Genetic features of blaNDM-1-positive Enterobacteriaceae.. Antimicrob Agents Chemother 55:, 5403–5407. [CrossRef][PubMed]
    [Google Scholar]
  21. Samuelsen O., Thilesen C. M., Heggelund L., Vada A. N., Kümmel A., Sundsfjord A.. ( 2011;). Identification of NDM-1-producing Enterobacteriaceae in Norway. . J Antimicrob Chemother 66:, 670–672. [CrossRef][PubMed]
    [Google Scholar]
  22. Sekizuka T., Matsui M., Yamane K., Takeuchi F., Ohnishi M., Hishinuma A., Arakawa Y., Kuroda M.. ( 2011;). Complete sequencing of the bla(NDM-1)-positive IncA/C plasmid from Escherichia coli ST38 isolate suggests a possible origin from plant pathogens. . PLoS ONE 6:, e25334. [CrossRef][PubMed]
    [Google Scholar]
  23. Tsakris A., Poulou A., Pournaras S., Voulgari E., Vrioni G., Themeli-Digalaki K., Petropoulou D., Sofianou D.. ( 2010;). A simple phenotypic method for the differentiation of metallo-β-lactamases and class A KPC carbapenemases in Enterobacteriaceae clinical isolates. . J Antimicrob Chemother 65:, 1664–1671. [CrossRef][PubMed]
    [Google Scholar]
  24. Villa L., Poirel L., Nordmann P., Carta C., Carattoli A.. ( 2012;). Complete sequencing of an IncH plasmid carrying the blaNDM-1, blaCTX-M-15 and qnrB1 genes. . J Antimicrob Chemother 67:, 1645–1650. [CrossRef][PubMed]
    [Google Scholar]
  25. Voulgari E., Zarkotou O., Ranellou K., Karageorgopoulos D. E., Vrioni G., Mamali V., Themeli-Digalaki K., Tsakris A.. ( 2013;). Outbreak of OXA-48 carbapenemase-producing Klebsiella pneumoniae in Greece involving an ST11 clone. . J Antimicrob Chemother 68:, 84–88. [CrossRef][PubMed]
    [Google Scholar]
  26. Williamson D. A., Sidjabat H. E., Freeman J. T., Roberts S. A., Silvey A., Woodhouse R., Mowat E., Dyet K., Paterson D. L. et al. ( 2012;). Identification and molecular characterisation of New Delhi metallo-β-lactamase-1 (NDM-1)- and NDM-6-producing Enterobacteriaceae from New Zealand hospitals. . Int J Antimicrob Agents 39:, 529–533. [CrossRef][PubMed]
    [Google Scholar]
  27. Wirth T., Falush D., Lan R., Colles F., Mensa P., Wieler L. H., Karch H., Reeves P. R., Maiden M. C. et al. ( 2006;). Sex and virulence in Escherichia coli: an evolutionary perspective. . Mol Microbiol 60:, 1136–1151. [CrossRef][PubMed]
    [Google Scholar]
  28. Yong D., Toleman M. A., Giske C. G., Cho H. S., Sundman K., Lee K., Walsh T. R.. ( 2009;). Characterization of a new metallo-β-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. . Antimicrob Agents Chemother 53:, 5046–5054. [CrossRef][PubMed]
    [Google Scholar]
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