1887

Abstract

Antimicrobial susceptibility, integron carriage, genetic relationship and presence of some important virulence genes of the integron-carrier strains of ( = 230) and ( = 22) isolated from stool samples of patients in Hungary between 1998 and 2008 were investigated. Sixty-seven per cent (168/252) of the strains were resistant to sulfamethoxazole/trimethoprim (SxT) followed by streptomycin (S, 47 %), ampicillin (A, 32 %) and tetracycline (Tc, 28 %). Thirty-six per cent (90/252) exhibited multidrug resistance, mostly showing SSxTTc or ASSxTc, ASSxTTc resistance patterns. An . strain of imported origin was resistant to cefotaxime and harboured a -type extended-spectrum β-lactamase gene. Altogether 33 % of the ( = 75) and 14 % of the ( = 3) strains had either class 1 or class 2 integrons or both. The variable regions encoded or genes for the class 1 and or genes for the class 2 integrons. Pulsed-field gel electrophoresis analysis revealed that those strains that have different integron types represented different genetic clusters. The Shiga toxin () gene was identified in one strain and the gene was detected in an strain. The results reveal the high incidence of antibiotic resistance among isolates and the presence of the gene in and the gene in . The genetic diversity of spp. isolated recently in Hungary was also demonstrated.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.058917-0
2013-10-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jmm/62/10/1545.html?itemId=/content/journal/jmm/10.1099/jmm.0.058917-0&mimeType=html&fmt=ahah

References

  1. Acikgoz Z. C., Eser O. K., Kocagöz S. 2008; CTX-M-3 type β-lactamase producing Shigella sonnei isolates from pediatric bacillary dysentery cases. Jpn J Infect Dis 61:135–137[PubMed]
    [Google Scholar]
  2. Ahmed A. M., Furuta K., Shimomura K., Kasama Y., Shimamoto T. 2006; Genetic characterization of multidrug resistance in Shigella spp. from Japan. J Med Microbiol 55:1685–1691 [View Article][PubMed]
    [Google Scholar]
  3. Cantey J. R. 1985; Shiga toxin – an expanding role in the pathogenesis of infectious diseases. J Infect Dis 151:766–771 [View Article][PubMed]
    [Google Scholar]
  4. China B., Pirson V., Mainil J. 1996; Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes. Appl Environ Microbiol 62:3462–3465[PubMed]
    [Google Scholar]
  5. CLSI 2005; Performance Standards for Antimicrobial Susceptibility Testing; Approved Standard, 9th edn, 15th Informational Supplement M100–S15. Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  6. Damjanova I., Tóth A., Pászti J., Hajbel-Vékony G., Jakab M., Berta J., Milch H., Füzi M. 2008; Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type β-lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005 – the new ‘MRSAs’?. J Antimicrob Chemother 62:978–985 [View Article][PubMed]
    [Google Scholar]
  7. Guichon A., Hersh D., Smith M. R., Zychlinsky A. 2001; Structure-function analysis of the Shigella virulence factor IpaB. J Bacteriol 183:1269–1276 [View Article][PubMed]
    [Google Scholar]
  8. Iversen J., Sandvang D., Srijan A., Cam P. D., Dalsgaard A. 2003; Characterization of antimicrobial resistance, plasmids, and gene cassettes in Shigella spp. from patients in Vietnam. Microb Drug Resist 9:Suppl 117–24 [View Article][PubMed]
    [Google Scholar]
  9. Johnson W. M., Lior H. 1987; Production of Shiga toxin and a cytolethal distending toxin (CLDT) by serogroups of Shigella spp. FEMS Microbiol Lett 48:235–238 [View Article]
    [Google Scholar]
  10. Kuo C. Y., Su L. H., Perera J., Carlos C., Tan B. H., Kumarasinghe G., So T., Van P. H., Chongthaleong A. other authors 2008; Antimicrobial susceptibility of Shigella isolates in eight Asian countries, 2001–2004. J Microbiol Immunol Infect 41:107–111[PubMed]
    [Google Scholar]
  11. Lévesque C., Piché L., Larose C., Roy P. H. 1995; PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob Agents Chemother 39:185–191 [View Article][PubMed]
    [Google Scholar]
  12. Lin Z., Kurazono H., Yamasaki S., Takeda Y. 1993; Detection of various variant verotoxin genes in Escherichia coli by polymerase chain reaction. Microbiol Immunol 37:543–548[PubMed] [CrossRef]
    [Google Scholar]
  13. McDonough M. A., Butterton J. R. 1999; Spontaneous tandem amplification and deletion of the shiga toxin operon in Shigella dysenteriae 1. Mol Microbiol 34:1058–1069 [View Article][PubMed]
    [Google Scholar]
  14. Moura A., Henriques I., Ribeiro R., Correia A. 2007; Prevalence and characterization of integrons from bacteria isolated from a slaughterhouse wastewater treatment plant. J Antimicrob Chemother 60:1243–1250 [View Article][PubMed]
    [Google Scholar]
  15. Nataro J. P., Kaper J. B. 1998; Diarrheagenic Escherichia coli . Clin Microbiol Rev 11:142–201[PubMed]
    [Google Scholar]
  16. Nógrády N., Gadó I., Tóth A., Pászti J. 2005; Antibiotic resistance and class 1 integron patterns of non-typhoidal human Salmonella serotypes isolated in Hungary in 2002 and 2003. Int J Antimicrob Agents 26:126–132 [View Article][PubMed]
    [Google Scholar]
  17. Nógrády N., Pászti J., Pikó H., Nagy B. 2006; Class 1 integrons and their conjugal transfer with and without virulence-associated genes in extra-intestinal and intestinal Escherichia coli of poultry. Avian Pathol 35:349–356 [View Article][PubMed]
    [Google Scholar]
  18. Oh J. Y., Yu H. S., Kim S. K., Seol S. Y., Cho D. T., Lee J. C. 2003; Changes in patterns of antimicrobial susceptibility and integron carriage among Shigella sonnei isolates from southwestern Korea during epidemic periods. J Clin Microbiol 41:421–423 [View Article][PubMed]
    [Google Scholar]
  19. Okuda J., Kurazono H., Takeda Y. 1995; Distribution of the cytolethal distending toxin A gene (cdtA) among species of Shigella and Vibrio, and cloning and sequencing of the cdt gene from Shigella dysenteriae . Microb Pathog 18:167–172 [View Article][PubMed]
    [Google Scholar]
  20. Pan J. C., Ye R., Meng D. M., Zhang W., Wang H. Q., Liu K. Z. 2006; Molecular characteristics of class 1 and class 2 integrons and their relationships to antibiotic resistance in clinical isolates of Shigella sonnei and Shigella flexneri . J Antimicrob Chemother 58:288–296 [View Article][PubMed]
    [Google Scholar]
  21. Peirano G., Agersø Y., Aarestrup F. M., dos Prazeres Rodrigues D. 2005; Occurrence of integrons and resistance genes among sulphonamide-resistant Shigella spp. from Brazil. J Antimicrob Chemother 55:301–305 [View Article][PubMed]
    [Google Scholar]
  22. Ribot E. M., Fair M. A., Gautom R., Cameron D. N., Hunter S. B., Swaminathan B., Barrett T. J. 2006; Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathog Dis 3:59–67 [View Article][PubMed]
    [Google Scholar]
  23. Solberg O. D., Ajiboye R. M., Riley L. W. 2006; Origin of class 1 and 2 integrons and gene cassettes in a population-based sample of uropathogenic Escherichia coli . J Clin Microbiol 44:1347–1351 [View Article][PubMed]
    [Google Scholar]
  24. Strauch E., Lurz R., Beutin L. 2001; Characterization of a Shiga toxin-encoding temperate bacteriophage of Shigella sonnei . Infect Immun 69:7588–7595 [View Article][PubMed]
    [Google Scholar]
  25. Tóth I., Hérault F., Beutin L., Oswald E. 2003; Production of cytolethal distending toxins by pathogenic Escherichia coli strains isolated from human and animal sources: establishment of the existence of a new cdt variant (Type IV). J Clin Microbiol 41:4285–4291 [View Article][PubMed]
    [Google Scholar]
  26. Tóth A., Nógrády N., Fekete P. Z., Pászti J., Füzi M. 2007; Extended-spectrum-β-lactamase-producing Salmonella enterica strains isolated from humans in Hungary, 2000 to 2004. J Antimicrob Chemother 59:579–582 [View Article][PubMed]
    [Google Scholar]
  27. White P. A., McIver C. J., Rawlinson W. D. 2001; Integrons and gene cassettes in the Enterobacteriaceae . Antimicrob Agents Chemother 45:2658–2661 [View Article][PubMed]
    [Google Scholar]
  28. Xiong Z., Li T., Xu Y., Li J. 2007; Detection of CTX-M-14 extended-spectrum β-lactamase in Shigella sonnei isolates from China. J Infect 55:125–128 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.058917-0
Loading
/content/journal/jmm/10.1099/jmm.0.058917-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error