1887

Abstract

Antimicrobial susceptibility, integron carriage, genetic relationship and presence of some important virulence genes of the integron-carrier strains of ( = 230) and ( = 22) isolated from stool samples of patients in Hungary between 1998 and 2008 were investigated. Sixty-seven per cent (168/252) of the strains were resistant to sulfamethoxazole/trimethoprim (SxT) followed by streptomycin (S, 47 %), ampicillin (A, 32 %) and tetracycline (Tc, 28 %). Thirty-six per cent (90/252) exhibited multidrug resistance, mostly showing SSxTTc or ASSxTc, ASSxTTc resistance patterns. An . strain of imported origin was resistant to cefotaxime and harboured a -type extended-spectrum β-lactamase gene. Altogether 33 % of the ( = 75) and 14 % of the ( = 3) strains had either class 1 or class 2 integrons or both. The variable regions encoded or genes for the class 1 and or genes for the class 2 integrons. Pulsed-field gel electrophoresis analysis revealed that those strains that have different integron types represented different genetic clusters. The Shiga toxin () gene was identified in one strain and the gene was detected in an strain. The results reveal the high incidence of antibiotic resistance among isolates and the presence of the gene in and the gene in . The genetic diversity of spp. isolated recently in Hungary was also demonstrated.

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2013-10-01
2020-01-26
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