Comparison of cytokine gene polymorphisms among Greek patients with invasive meningococcal disease or viral meningitis Free

Abstract

High levels of pro-inflammatory cytokines are implicated in the severity of invasive meningococcal disease (IMD) and viral meningitis (VM). This study compared single-nucleotide polymorphisms (SNPs) in pro- and anti-inflammatory cytokine genes among patients with VM or IMD. Patient DNA samples were prepared by the National Meningitis Reference Laboratory in Athens:  = 98 for IMD and  = 53 for VM. The results for both patient groups were compared with data published for healthy Greek control data. Real-time PCR was used to assess the interleukin (IL) gene SNPs G−174C, C−511T, T+2018C, G−1082A and A−251T and the tumour necrosis factor α (TNF-α) SNP G−308A. Differences were compared by Fisher’s exact test. The genotype for high IL-6 responses was predominant among IMD (51 %,  = 0.0008) and VM (74.5 %, <0.0001) patients compared with the controls (31 %). The genotype associated with high TNF-α responses was 5 % among controls and lower for IMD (1.1 %,  = 0.0014) and VM (0 %,  = 0.052). There was no difference for IL-8 SNPs between controls and IMD ( = 0.162), but the difference was significant for VM ( = 0.0025). IL-6 ( = 0.024) and IL-8 ( = 0.00004) SNPs differed between IMD and VM. Reports on associations between IL-8 SNPs and cytokine responses differ. Because of its role in neutrophil attraction, differences in frequencies of the IL-8 SNP for IMD and VM require further investigation.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.058073-0
2013-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/62/5/694.html?itemId=/content/journal/jmm/10.1099/jmm.0.058073-0&mimeType=html&fmt=ahah

References

  1. Allen R. D. 1999; Polymorphism of the human TNF-α promoter – random variation or functional diversity?. Mol Immunol 36:1017–1027 [View Article][PubMed]
    [Google Scholar]
  2. Azuma H., Tsuda N., Sasaki K., Okuno A. 1997; Clinical significance of cytokine measurement for detection of meningitis. J Pediatr 131:463–465 [View Article][PubMed]
    [Google Scholar]
  3. Balding J., Healy C. M., Livingstone W. J., White B., Mynett-Johnson L., Cafferkey M., Smith O. P. 2003; Genomic polymorphic profiles in an Irish population with meningococcaemia: is it possible to predict severity and outcome of disease?. Genes Immun 4:533–540 [View Article][PubMed]
    [Google Scholar]
  4. Blackwell C. C., Moscovis S. M., Gordon A. E., Al Madani O. M., Hall S. T., Gleeson M., Scott R. J., Roberts-Thomson J., Weir D. M., Busuttil A. 2005; Cytokine responses and sudden infant death syndrome: genetic, developmental, and environmental risk factors. J Leukoc Biol 78:1242–1254 [View Article][PubMed]
    [Google Scholar]
  5. Christodoulides M., Makepeace B. L., Partridge K. A., Kaur D., Fowler M. I., Weller R. O., Heckels J. E. 2002; Interaction of Neisseria meningitidis with human meningeal cells induces the secretion of a distinct group of chemotactic, proinflammatory, and growth-factor cytokines. Infect Immun 70:4035–4044 [View Article][PubMed]
    [Google Scholar]
  6. Costeas P. A., Koumas L., Koumouli A., Kyriakou-Giantsiou A., Papaloizou A. 2003; Cytokine polymorphism frequencies in the Greek Cypriot population. Eur J Immunogenet 30:341–343 [View Article][PubMed]
    [Google Scholar]
  7. Dalal I., Tzhori S., Somekh E., Mandelberg A., Levine A., Ballin A. 2003; Cytokine profile in cerebrospinal fluid of children with echovirus type 4 meningitis. Pediatr Neurol 29:312–316 [View Article][PubMed]
    [Google Scholar]
  8. Dulkerian S. J., Kilpatrick L., Costarino A. T. Jr, McCawley L., Fein J., Corcoran L., Zirin S., Harris M. C. 1995; Cytokine elevations in infants with bacterial and aseptic meningitis. J Pediatr 126:872–876 [View Article][PubMed]
    [Google Scholar]
  9. Fida N. M., Al-Mughales J., Farouq M. 2006; Interleukin-1α, interleukin-6 and tumor necrosis factor-α levels in children with sepsis and meningitis. Pediatr Int 48:118–124 [View Article][PubMed]
    [Google Scholar]
  10. Fishman D., Faulds G., Jeffery R., Mohamed-Ali V., Yudkin J. S., Humphries S., Woo P. 1998; The effect of novel polymorphisms in the interleukin-6 (IL-6) gene on IL-6 transcription and plasma IL-6 levels, and an association with systemic-onset juvenile chronic arthritis. J Clin Invest 102:1369–1376 [View Article][PubMed]
    [Google Scholar]
  11. Hacking D., Knight J. C., Rockett K., Brown H., Frampton J., Kwiatkowski D. P., Hull J., Udalova I. A. 2004; Increased in vivo transcription of an IL-8 haplotype associated with respiratory syncytial virus disease-susceptibility. Genes Immun 5:274–282 [View Article][PubMed]
    [Google Scholar]
  12. Hull J., Thomson A., Kwiatkowski D. 2000; Association of respiratory syncytial virus bronchiolitis with the interleukin 8 gene region in UK families. Thorax 55:1023–1027 [View Article][PubMed]
    [Google Scholar]
  13. Hull J., Ackerman H., Isles K., Usen S., Pinder M., Thomson A., Kwiatkowski D. P. 2001; Unusual haplotypic structure of IL8, a susceptibility locus for a common respiratory virus. Am J Hum Genet 69:413–419 [View Article][PubMed]
    [Google Scholar]
  14. Ishiguro A., Suzuki Y., Inaba Y., Fukushima K., Komiyama A., Koeffler H. P., Shimbo T. 1997; The production of IL-8 in cerebrospinal fluid in aseptic meningitis of children. Clin Exp Immunol 109:426–430 [View Article][PubMed]
    [Google Scholar]
  15. Jiang Z. D., Okhuysen P. C., Guo D. C., He R., King T. M., DuPont H. L., Milewicz D. M. 2003; Genetic susceptibility to enteroaggregative Escherichia coli diarrhea: polymorphism in the interleukin-8 promotor region. J Infect Dis 188:506–511 [View Article][PubMed]
    [Google Scholar]
  16. Krebs V. L., Okay T. S., Okay Y., Vaz F. A. 2005; Tumor necrosis factor-α, interleukin-1β and interleukin-6 in the cerebrospinal fluid of newborn with meningitis. Arq Neuropsiquiatr 63:7–13 [View Article][PubMed]
    [Google Scholar]
  17. Lee W.-P., Tai D.-I., Lan K.-H., Li A.-F., Hsu H.-C., Lin E.-J., Lin Y.-P., Sheu M.-L., Li C.-P. et al. 2005; The −251T allele of the interleukin-8 promoter is associated with increased risk of gastric carcinoma featuring diffuse-type histopathology in Chinese population. Clin Cancer Res 11:6431–6441 [View Article][PubMed]
    [Google Scholar]
  18. Louis E., Franchimont D., Piron A., Gevaert Y., Schaaf-Lafontaine N., Roland S., Mahieu P., Malaise M., De Groote D. et al. 1998; Tumour necrosis factor (TNF) gene polymorphism influences TNF-α production in lipopolysaccharide (LPS)-stimulated whole blood cell culture in healthy humans. Clin Exp Immunol 113:401–406 [View Article][PubMed]
    [Google Scholar]
  19. Massey P., Durrheim D. 2008; Aboriginal and Torres Strait Islander peoples at higher risk of invasive meningococcal disease in NSW. N S W Public Health Bull 19:100–103 [View Article][PubMed]
    [Google Scholar]
  20. Matsuzono Y., Narita M., Akutsu Y., Togashi T. 1995; Interleukin-6 in cerebrospinal fluid of patients with central nervous system infections. Acta Paediatr 84:879–883 [View Article][PubMed]
    [Google Scholar]
  21. Møller K., Tofteng F., Qvist T., Sahl C., Sønderkaer S., Pedersen B. K. 2005; Cerebral output of cytokines in patients with pneumococcal meningitis. Crit Care Med 33:979–983 [View Article][PubMed]
    [Google Scholar]
  22. Moscovis S. M., Gordon A. E., Al Madani O. M., Gleeson M., Scott R. J., Roberts-Thomson J., Hall S. T., Weir D. M., Busuttil A., Blackwell C. C. 2004a; Interleukin-10 and sudden infant death syndrome. FEMS Immunol Med Microbiol 42:130–138 [View Article][PubMed]
    [Google Scholar]
  23. Moscovis S. M., Gordon A. E., Al Madani O. M., Gleeson M., Scott R. J., Roberts-Thomson J., Hall S. T., Weir D. M., Busuttil A., Blackwell C. C. 2004b; Interleukin-1β and sudden infant death syndrome. FEMS Immunol Med Microbiol 42:139–145 [View Article][PubMed]
    [Google Scholar]
  24. Moscovis S. M., Gordon A. E., Al Madani O. M., Gleeson M., Scott R. J., Roberts-Thomson J., Hall S. T., Weir D. M., Busuttil A., Blackwell C. C. 2006; IL6 G−174C associated with sudden infant death syndrome in a Caucasian Australian cohort. Hum Immunol 67:819–825 [View Article][PubMed]
    [Google Scholar]
  25. Mukai A. O., Krebs V. L., Bertoli C. J., Okay T. S. 2006; TNF-α and IL-6 in the diagnosis of bacterial and aseptic meningitis in children. Pediatr Neurol 34:25–29 [View Article][PubMed]
    [Google Scholar]
  26. Ostergaard C., Benfield T. L., Sellebjerg F., Kronborg G., Lohse N., Lundgren J. D. 1996; Interleukin-8 in cerebrospinal fluid from patients with septic and aseptic meningitis. Eur J Clin Microbiol Infect Dis 15:166–169 [View Article][PubMed]
    [Google Scholar]
  27. Read R. C., Camp N. J., di Giovine F. S., Borrow R., Kaczmarski E. B., Chaudhary A. G., Fox A. J., Duff G. W. 2000; An interleukin-1 genotype is associated with fatal outcome of meningococcal disease. J Infect Dis 182:1557–1560 [View Article][PubMed]
    [Google Scholar]
  28. Read R. C., Cannings C., Naylor S. C., Timms J. M., Maheswaran R., Borrow R., Kaczmarski E. B., Duff G. W. 2003; Variation within genes encoding interleukin-1 and the interleukin-1 receptor antagonist influence the severity of meningococcal disease. Ann Intern Med 138:534–541[PubMed] [CrossRef]
    [Google Scholar]
  29. Sato M., Hosoya M., Honzumi K., Watanabe M., Ninomiya N., Shigeta S., Suzuki H. 2003; Cytokine and cellular inflammatory sequence in enteroviral meningitis. Pediatrics 112:1103–1107 [View Article][PubMed]
    [Google Scholar]
  30. Selvaraj P., Prabhu Anand S., Jawahar M. S., Chandra G., Banurekha B., Narayanan P. R. 2006; Promoter polymorphism of IL-8 gene and IL-8 production in pulmonary tuberculosis. Curr Sci 90:952–954
    [Google Scholar]
  31. Straussberg R., Harel L., Nussinovitch M., Amir J. 2003; Absolute neutrophil count in aseptic and bacterial meningitis related to time of lumbar puncture. Pediatr Neurol 28:365–369 [View Article][PubMed]
    [Google Scholar]
  32. Taguchi A., Ohmiya N., Shirai K., Mabuchi N., Itoh A., Hirooka Y., Niwa Y., Goto H. 2005; Interleukin-8 promoter polymorphism increases the risk of atrophic gastritis and gastric cancer in Japan. Cancer Epidemiol Biomarkers Prev 14:2487–2493 [View Article][PubMed]
    [Google Scholar]
  33. Tang R. B., Lee B. H., Chung R. L., Chen S. J., Wong T. T. 2001; Interleukin-1β and tumor necrosis factor-α in cerebrospinal fluid of children with bacterial meningitis. Childs Nerv Syst 17:453–456 [View Article][PubMed]
    [Google Scholar]
  34. Theodoropoulos G., Papaconstantinou I., Felekouras E., Nikiteas N., Karakitsos P., Panoussopoulos D., Lazaris A. Ch., Patsouris E., Bramis J., Gazouli M. 2006; Relation between common polymorphisms in genes related to inflammatory response and colorectal cancer. World J Gastroenterol 12:5037–5043[PubMed]
    [Google Scholar]
  35. Tsolia M. N., Theodoridou M., Tzanakaki G., Kalabalikis P., Urani E., Mostrou G., Pangalis A., Zafiropoulou A., Kassiou C. et al. 2003; The evolving epidemiology of invasive meningococcal disease: a two-year prospective, population-based study in children in the area of Athens. FEMS Immunol Med Microbiol 36:87–94 [View Article][PubMed]
    [Google Scholar]
  36. Tzanakaki G., Tsolia M., Vlachou V., Theodoridou M., Pangalis A., Foustoukou M., Karpathios T., Blackwell C. C., Kremastinou J. 2003; Evaluation of non-culture diagnosis of invasive meningococcal disease by polymerase chain reaction (PCR). FEMS Immunol Med Microbiol 39:31–36 [View Article][PubMed]
    [Google Scholar]
  37. Venkatesh B., Scott P., Ziegenfuss M. 2000; Cerebrospinal fluid in critical illness. Crit Care Resusc 2:42–54[PubMed]
    [Google Scholar]
  38. Villuendas G., San Millán J. L., Sancho J., Escobar-Morreale H. F. 2002; The −597 G→A and −174 G→C polymorphisms in the promoter of the IL-6 gene are associated with hyperandrogenism. J Clin Endocrinol Metab 87:1134–1141 [View Article][PubMed]
    [Google Scholar]
  39. Westendorp R. G., Langermans J. A., Huizinga T. W., Elouali A. H., Verweij C. L., Boomsma D. I., Vandenbroucke J. P. 1997; Genetic influence on cytokine production and fatal meningococcal disease. Lancet 349:170–173 [View Article][PubMed]
    [Google Scholar]
  40. Wilson A. G., Symons J. A., McDowell T. L., McDevitt H. O., Duff G. W. 1997; Effects of a polymorphism in the human tumor necrosis factor α promoter on transcriptional activation. Proc Natl Acad Sci U S A 94:3195–3199 [View Article][PubMed]
    [Google Scholar]
  41. Zambardi G., Druetta A., Roure C., Fouqué B., Girardo P., Chypre C., Marchand J., Freney J., Fleurette J. 1995; Rapid diagnosis of Mycobacterium tuberculosis infections by an ELISA-like detection of polymerase chain reaction products. Mol Cell Probes 9:91–99 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.058073-0
Loading
/content/journal/jmm/10.1099/jmm.0.058073-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed