1887

Abstract

This study reports an infectious case involving an NDM-1-producing and further explored the potential threat of the gene by analysing the characteristics of the NDM-1-encoding plasmid sequence. A -positive with high resistance to carbapenems was separated from a clinical patient suffering from a urinary tract infection. S1 nuclease-based plasmid analysis followed by Southern blot hybridization, a conjugation experiment and electrotransformation confirmed that the gene was located on a plasmid. High-throughput sequencing of the -postive plasmid (pCFNDM-CN) showed that it was a 54 kb IncX-type plasmid and contained a backbone region and a variable region with two β-lactamase genes ( and ). The NDM-1 composite transposon in the variable region was surrounded by IS and IS-truncated IS, and shared a high sequence similarity to the surrounding structure in spp. Our research suggested that the NDM-1 composite transposon might play an essential role in mobilization of the gene from spp. to Enterobacteriaceae.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.057091-0
2013-09-01
2020-07-02
Loading full text...

Full text loading...

/deliver/fulltext/jmm/62/9/1332.html?itemId=/content/journal/jmm/10.1099/jmm.0.057091-0&mimeType=html&fmt=ahah

References

  1. Bae I. K., Jang S. J., Kim J., Jeong S. H., Cho B., Lee K. 2011; Interspecies dissemination of the bla gene encoding PER-1 extended-spectrum β-lactamase. Antimicrob Agents Chemother 55:1305–1307 [CrossRef][PubMed]
    [Google Scholar]
  2. Bonnin R. A., Poirel L., Naas T., Pirs M., Seme K., Schrenzel J., Nordmann P. 2012; Dissemination of New Delhi metallo-β-lactamase-1-producing Acinetobacter baumannii in Europe. Clin Microbiol Infect 18:E362–E365 [CrossRef][PubMed]
    [Google Scholar]
  3. Carattoli A., Bertini A., Villa L., Falbo V., Hopkins K. L., Threlfall E. J. 2005; Identification of plasmids by PCR-based replicon typing. J Microbiol Methods 63:219–228 [CrossRef][PubMed]
    [Google Scholar]
  4. Chen Y., Zhou Z., Jiang Y., Yu Y. 2011; Emergence of NDM-1-producing Acinetobacter baumannii in China. J Antimicrob Chemother 66:1255–1259 [CrossRef][PubMed]
    [Google Scholar]
  5. CLSI 2012; Performance Standards for Antimicrobial Susceptibility Testing; 22nd Informational Supplement M100-S22. Wayne, PA: Clinical and Laboratory Standards Institute;
  6. Dolejska M., Villa L., Poirel L., Nordmann P., Carattoli A. 2013; Complete sequencing of an IncHI1 plasmid encoding the carbapenemase NDM-1, the ArmA 16S RNA methylase and a resistance–nodulation–cell division/multidrug efflux pump. J Antimicrob Chemother 68:34–39 [CrossRef][PubMed]
    [Google Scholar]
  7. Fu Y., Du X., Ji J., Chen Y., Jiang Y., Yu Y. 2012; Epidemiological characteristics and genetic structure of blaNDM-1 in non-baumannii Acinetobacter spp. in China. J Antimicrob Chemother 67:2114–2122 [CrossRef][PubMed]
    [Google Scholar]
  8. Gupta N., Limbago B. M., Patel J. B., Kallen A. J. 2011; Carbapenem-resistant Enterobacteriaceae: epidemiology and prevention. Clin Infect Dis 53:60–67 [CrossRef][PubMed]
    [Google Scholar]
  9. Ho P. L., Lo W. U., Yeung M. K., Lin C. H., Chow K. H., Ang I., Tong A. H., Bao J. Y., Lok S., Lo J. Y. 2011; Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong. PLoS ONE 6:e17989 [CrossRef][PubMed]
    [Google Scholar]
  10. Ho P. L., Li Z., Lo W. U., Cheung Y. Y., Lin C. H., Sham P. C., Cheng V. C., Ng T. K., Que T. L., Chow K. H. 2012; Identification and characterization of a novel incompatibility group X3 plasmid carrying blaNDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China. Emerg Microbes Infect 1:e39 [CrossRef]
    [Google Scholar]
  11. Hu H., Hu Y., Pan Y., Liang H., Wang H., Wang X., Hao Q., Yang X., Xiao X., Luan C. other authors 2012; A novel plasmid and its variant harboring both blaNDM-1 gene and T4SS in clinical isolates of Acinetobacter lwoffii. Antimicrob Agents Chemother 56:1698–1702 [CrossRef][PubMed]
    [Google Scholar]
  12. Johnson T. J., Bielak E. M., Fortini D., Hansen L. H., Hasman H., Debroy C., Nolan L. K., Carattoli A. 2012; Expansion of the IncX plasmid family for improved identification and typing of novel plasmids in drug-resistant Enterobacteriaceae. Plasmid 68:43–50 [CrossRef][PubMed]
    [Google Scholar]
  13. Kumarasamy K. K., Toleman M. A., Walsh T. R., Bagaria J., Butt F., Balakrishnan R., Chaudhary U., Doumith M., Giske C. G. other authors 2010; Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis 10:597–602 [CrossRef][PubMed]
    [Google Scholar]
  14. Lee K., Yum J. H., Yong D., Lee H. M., Kim H. D., Docquier J. D., Rossolini G. M., Chong Y. 2005; Novel acquired metallo-β-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from Korea. Antimicrob Agents Chemother 49:4485–4491 [CrossRef][PubMed]
    [Google Scholar]
  15. Lee M. F., Peng C. F., Hsu H. J., Chen Y. H. 2008; Molecular characterisation of the metallo-β-lactamase genes in imipenem-resistant Gram-negative bacteria from a university hospital in southern Taiwan. Int J Antimicrob Agents 32:475–480 [CrossRef][PubMed]
    [Google Scholar]
  16. Literak I., Dolejska M., Janoszowska D., Hrusakova J., Meissner W., Rzyska H., Bzoma S., Cizek A. 2010; Antibiotic-resistant Escherichia coli bacteria, including strains with genes encoding the extended-spectrum β-lactamase and QnrS, in waterbirds on the Baltic Sea Coast of Poland. Appl Environ Microbiol 76:8126–8134 [CrossRef][PubMed]
    [Google Scholar]
  17. Moellering R. C. Jr 2010; NDM-1 – a cause for worldwide concern. N Engl J Med 363:2377–2379 [CrossRef][PubMed]
    [Google Scholar]
  18. Pfeifer Y., Wilharm G., Zander E., Wichelhaus T. A., Göttig S., Hunfeld K. P., Seifert H., Witte W., Higgins P. G. 2011; Molecular characterization of blaNDM-1 in an Acinetobacter baumannii strain isolated in Germany in 2007. J Antimicrob Chemother 66:1998–2001 [CrossRef][PubMed]
    [Google Scholar]
  19. Poirel L., Dortet L., Bernabeu S., Nordmann P. 2011a; Genetic features of blaNDM-1-positive Enterobacteriaceae. Antimicrob Agents Chemother 55:5403–5407 [CrossRef][PubMed]
    [Google Scholar]
  20. Poirel L., Bonnin R. A., Nordmann P. 2011b; Analysis of the resistome of a multidrug-resistant NDM-1-producing Escherichia coli strain by high-throughput genome sequencing. Antimicrob Agents Chemother 55:4224–4229 [CrossRef][PubMed]
    [Google Scholar]
  21. Sekizuka T., Matsui M., Yamane K., Takeuchi F., Ohnishi M., Hishinuma A., Arakawa Y., Kuroda M. 2011; Complete sequencing of the blaNDM-1-positive IncA/C plasmid from Escherichia coli ST38 isolate suggests a possible origin from plant pathogens. PLoS ONE 6:e25334 [CrossRef][PubMed]
    [Google Scholar]
  22. Solé M., Pitart C., Roca I., Fàbrega A., Salvador P., Muñoz L., Oliveira I., Gascón J., Marco F., Vila J. 2011; First description of an Escherichia coli strain producing NDM-1 carbapenemase in Spain. Antimicrob Agents Chemother 55:4402–4404 [CrossRef][PubMed]
    [Google Scholar]
  23. Struelens M. J., Monnet D. L., Magiorakos A. P., Santos O’Connor F., Giesecke J.European NDM-1 Survey Participants 2010; New Delhi metallo-β-lactamase 1-producing Enterobacteriaceae: emergence and response in Europe. Euro Surveill 15:19716[PubMed]
    [Google Scholar]
  24. Walsh T. R., Weeks J., Livermore D. M., Toleman M. A. 2011; Dissemination of NDM-1 positive bacteria in the New Delhi environment and its implications for human health: an environmental point prevalence study. Lancet Infect Dis 11:355–362 [CrossRef][PubMed]
    [Google Scholar]
  25. Wu H. S., Chen T. L., Chen I. C., Huang M. S., Wang F. D., Fung C. P., Lee S. D. 2010; First identification of a patient colonized with Klebsiella pneumoniae carrying blaNDM-1 in Taiwan. J Chin Med Assoc 73:596–598 [CrossRef][PubMed]
    [Google Scholar]
  26. Yong D., Toleman M. A., Giske C. G., Cho H. S., Sundman K., Lee K., Walsh T. R. 2009; Characterization of a new metallo-β-lactamase gene, blaNDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother 53:5046–5054 [CrossRef][PubMed]
    [Google Scholar]
  27. Zerbino D. R., Birney E. 2008; Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18:821–829 [CrossRef][PubMed]
    [Google Scholar]
  28. Zhang R., Zhou H. W., Cai J. C., Zhang J., Chen G. X., Nasu M., Xie X. Y. 2011; Serotypes and extended-spectrum β-lactamase types of clinical isolates of Shigella spp. from the Zhejiang province of China. Diagn Microbiol Infect Dis 69:98–104 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.057091-0
Loading
/content/journal/jmm/10.1099/jmm.0.057091-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error