RT Journal Article SR Electronic(1) A1 Shi, Yanyan A1 Liu, Linna A1 Zhang, Ting A1 Shen, Lijuan A1 Liu, Lin A1 Zhang, Jing A1 Zhang, Yuexia A1 Wang, Xuehong A1 Yang, Shengsen A1 Lu, Fengmin A1 Chen, Xiangmei A1 Ding, ShigangYR 2013 T1 The involvement of Helicobacter pylori thioredoxin-1 in gastric carcinogenesis JF Journal of Medical Microbiology, VO 62 IS 8 SP 1226 OP 1234 DO https://doi.org/10.1099/jmm.0.056903-0 PB Microbiology Society, SN 1473-5644, AB Helicobacter pylori infection is related to the development of gastric diseases. Various virulence factors are responsible for the pathogenic mechanisms of H. pylori infection. Our previous studies using two-dimensional gel electrophoresis showed that H. pylori thioredoxin-1 (Trx1) is overexpressed in gastric carcinomas. Here, we examined whether H. pylori Trx1 is a novel virulence factor associated with gastric tumorigenesis. We found that Trx1 expression in H. pylori isolated from gastric cancer tissues was significantly higher than that from tissues exhibiting gastritis. In the gastric epithelial cell line GES-1, infection of H. pylori with high Trx1 expression significantly induced cell apoptosis, decreased the expression of cyclin D1 and upregulated p21. However, in the gastric cancer cell line BGC823, high Trx1 expression in H. pylori significantly increased cell proliferation, and upregulated cyclin D1. The effects on cell lines were confirmed using the H. pylori Trx1-knockout mutant strain. Our observations indicate that high Trx1 expression in H. pylori is associated with gastric carcinogenesis. In H. pylori, Trx1 likely participates in the pathogenesis of gastric cancer and H. pylori expressing high levels of Trx1 would be expected to be highly pathogenic in gastric diseases in China., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.056903-0