1887

Abstract

The presence of in food like shellfish, vegetables and meat has been reported in several publications during the past few years. The objective of this study was to assess the prevalence of ready-to-eat raw vegetables contaminated with in France. One hundred and four ready-to-eat salads and vegetables were studied. Toxigenic strains were isolated in three samples (2.9 %): two ready-to-eat salads (one heart of lettuce and one lamb’s lettuce salad) and one portion of pea sprouts. The strains belonged to three different PCR ribotypes: 001, 014/020/077 and 015. The detection thresholds for vegetative cells and spores cells varied between 1 and 3 c.f.u. in 20 g salad and between 6 and 15 c.f.u. in 20 g salad, respectively, for the method employed.

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2013-09-01
2019-10-20
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References

  1. Al Saif N. , Brazier J. S. . ( 1996; ). The distribution of Clostridium difficile in the environment of South Wales. . J Med Microbiol 45:, 133–137. [CrossRef] [PubMed]
    [Google Scholar]
  2. Avbersek J. , Janezic S. , Pate M. , Rupnik M. , Zidaric V. , Logar K. , Vengust M. , Zemljic M. , Pirs T. , Ocepek M. . ( 2009; ). Diversity of Clostridium difficile in pigs and other animals in Slovenia. . Anaerobe 15:, 252–255. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bakri M. M. , Brown D. J. , Butcher J. P. , Sutherland A. D. . ( 2009; ). Clostridium difficile in ready-to-eat salads, Scotland. . Emerg Infect Dis 15:, 817–818. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bauer M. P. , Veenendaal D. , Verhoef L. , Bloembergen P. , van Dissel J. T. , Kuijper E. J. . ( 2009; ). Clinical and microbiological characteristics of community-onset Clostridium difficile infection in The Netherlands. . Clin Microbiol Infect 15:, 1087–1092. [CrossRef] [PubMed]
    [Google Scholar]
  5. Bauer M. P. , Notermans D. W. , van Benthem B. H. , Brazier J. S. , Wilcox M. H. , Rupnik M. , Monnet D. L. , van Dissel J. T. , Kuijper E. J. . ECDIS Study Group ( 2011; ). Clostridium difficile infection in Europe: a hospital-based survey. . Lancet 377:, 63–73. [CrossRef] [PubMed]
    [Google Scholar]
  6. Bidet P. , Barbut F. , Lalande V. , Burghoffer B. , Petit J. C. . ( 1999; ). Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing. . FEMS Microbiol Lett 175:, 261–266. [CrossRef] [PubMed]
    [Google Scholar]
  7. Bouttier S. , Barc M. C. , Felix B. , Lambert S. , Collignon A. , Barbut F. . ( 2010; ). Clostridium difficile in ground meat, France. . Emerg Infect Dis 16:, 733–735. [CrossRef] [PubMed]
    [Google Scholar]
  8. Debast S. B. , van Leengoed L. A. , Goorhuis A. , Harmanus C. , Kuijper E. J. , Bergwerff A. A. . ( 2009; ). Clostridium difficile PCR ribotype 078 toxinotype V found in diarrhoeal pigs identical to isolates from affected humans. . Environ Microbiol 11:, 505–511. [CrossRef] [PubMed]
    [Google Scholar]
  9. Eckert C. , Coignard B. , Hebert M. , Tarnaud C. , Tessier C. , Lemire A. , Burghoffer B. , Noel D. , Barbut F. . ( 2013; ). Clinical and microbiological features of Clostridium difficile infections in France: the ICD-RAISIN 2009 national survey. . Med Mal Infect 43:, 67–74.[CrossRef]
    [Google Scholar]
  10. Goorhuis A. , Bakker D. , Corver J. , Debast S. B. , Harmanus C. , Notermans D. W. , Bergwerff A. A. , Dekker F. W. , Kuijper E. J. . ( 2008a; ). Emergence of Clostridium difficile infection due to a new hypervirulent strain, polymerase chain reaction ribotype 078. . Clin Infect Dis 47:, 1162–1170. [CrossRef] [PubMed]
    [Google Scholar]
  11. Goorhuis A. , Debast S. B. , van Leengoed L. A. , Harmanus C. , Notermans D. W. , Bergwerff A. A. , Kuijper E. J. , Songer J. G. . ( 2008b; ). Clostridium difficile PCR ribotype 078: an emerging strain in humans and in pigs?. J Clin Microbiol 46:, 1157–1158 (author reply, 1158). [CrossRef] [PubMed]
    [Google Scholar]
  12. Hensgens M. P. M. , Keessen E. C. , Squire M. M. , Riley T. V. , Koene M. G. J. , de Boer E. , Lipman L. J. A. , Kuijper E. J. . European Society of Clinical Microbiology and Infectious Diseases Study Group for Clostridium difficile (ESGCD) ( 2012; ). Clostridium difficile infection in the community: a zoonotic disease?. Clin Microbiol Infect 18:, 635–645. [CrossRef] [PubMed]
    [Google Scholar]
  13. Keel K. , Brazier J. S. , Post K. W. , Weese S. , Songer J. G. . ( 2007; ). Prevalence of PCR ribotypes among Clostridium difficile isolates from pigs, calves, and other species. . J Clin Microbiol 45:, 1963–1964. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kuijper E. J. , Coignard B. , Tüll P. . ESCMID Study Group for Clostridium difficile EU Member States European Centre for Disease Prevention and Control ( 2006; ). Emergence of Clostridium difficile-associated disease in North America and Europe. . Clin Microbiol Infect 12: (Suppl 6), 2–18. [CrossRef] [PubMed]
    [Google Scholar]
  15. Loo V. G. , Poirier L. , Miller M. A. , Oughton M. , Libman M. D. , Michaud S. , Bourgault A. M. , Nguyen T. , Frenette C. . & other authors ( 2005; ). A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality. . N Engl J Med 353:, 2442–2449. [CrossRef] [PubMed]
    [Google Scholar]
  16. McDonald L. C. , Killgore G. E. , Thompson A. , Owens R. C. Jr , Kazakova S. V. , Sambol S. P. , Johnson S. , Gerding D. N. . ( 2005; ). An epidemic, toxin gene-variant strain of Clostridium difficile . . N Engl J Med 353:, 2433–2441. [CrossRef] [PubMed]
    [Google Scholar]
  17. Metcalf D. S. , Costa M. C. , Dew W. M. V. , Weese J. S. . ( 2010; ). Clostridium difficile in vegetables, Canada. . Lett Appl Microbiol 51:, 600–602. [CrossRef] [PubMed]
    [Google Scholar]
  18. Riley T. V. , Adams J. E. , O’Neill G. L. , Bowman R. A. . ( 1991; ). Gastrointestinal carriage of Clostridium difficile in cats and dogs attending veterinary clinics. . Epidemiol Infect 107:, 659–665. [CrossRef] [PubMed]
    [Google Scholar]
  19. Rupnik M. , Widmer A. , Zimmermann O. , Eckert C. , Barbut F. . ( 2008; ). Clostridium difficile toxinotype V, ribotype 078, in animals and humans. . J Clin Microbiol 46:, 2146. [CrossRef] [PubMed]
    [Google Scholar]
  20. Wilcox M. H. , Mooney L. , Bendall R. , Settle C. D. , Fawley W. N. . ( 2008; ). A case-control study of community-associated Clostridium difficile infection. . J Antimicrob Chemother 62:, 388–396. [CrossRef] [PubMed]
    [Google Scholar]
  21. Zidaric V. , Rupnik M. . ( 2012; ). Clostridium difficile in meat products, eggs and vegetables in Slovenia. . In 4th International Clostridium difficile Symposium, 20–22 September, 2012, Bled, Slovenia:, abstract: Poster 118.
    [Google Scholar]
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