1887

Abstract

In ocular infections (OIs) caused by , biofilms composed mainly of poly--acetylglucosamine (PNAG) have been widely studied, but PNAG-independent biofilms have not. Therefore, we searched for a relationship between the operon (involved in PNAG-biofilm) and the biochemical composition of biofilms in isolates from OI. Isolates from OI ( = 62), from healthy conjunctiva (HC;  = 45) and from healthy skin (HS;  = 53), were used to detect and genes, and the insertion sequence 256 (IS) using PCR. The compositions of the biofilms were determined by treatment with NaIO, proteinase K and DNase I. Multilocus sequence typing (MLST) was performed to characterize the isolates, and the expression of and genes was determined by real-time qPCR. A strong relationship between the / /IS genotype and protein- or protein/extracellular DNA (eDNA)-biofilm composition was found in the isolates from OI (53.6 %), whereas the / /IS genotype and carbohydrate-biofilm was most prevalent in isolates from HC (25 %) and HS (25 %). Isolates with an / /IS genotype and protein-biofilm phenotype were predominantly of the ST2 lineage, while carbohydrate-biofilm-producing strains were mainly of the ST9 lineage. The protein-biofilm-producing strains had higher expression levels of gene than carbohydrate-biofilm-producing strains; while gene did not have the same pattern of expression. These results suggest that strains with //IS genotype and protein- or protein/eDNA-biofilms have a stronger ability to establish in the eye than strains with //IS genotype and PNAG-biofilms.

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2013-10-01
2020-10-26
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