1887

Abstract

Therapeutic activity against oral candidiasis of orally administered bovine lactoferrin (LF), a multifunctional milk protein, was shown in a previous report using an immunosuppressed murine model. In the present study, the influence of orally administered LF on immune responses relevant to this therapeutic effect was examined. Because mice were immunosuppressed with prednisolone 1 day before and 3 days after the infection with , the numbers of peripheral blood leukocytes (PBL) and cervical lymph node (CLN) cells were reduced. LF feeding prevented the reduction in the numbers of PBL on day 1 and CLN cells on days 1, 5 and 6 in the -infected mice. The number of CLN cells of individual mice on days 5 and 6 was inversely correlated with the c.f.u. in the oral cavity. Increased production of IFN-γ and TNF-α by CLN cells stimulated with heat-killed on day 6 was observed in LF-treated mice compared with non-treated mice. Concanavalin A (ConA)-stimulated CLN cells from LF-treated mice also showed a significant increase in the production of IFN-γ and IL12 on day 5 and a tendency for increased production of IFN-γ and TNF-α on day 6. The levels of cytokine production by ConA-stimulated CLN cells on day 6 were inversely correlated with the c.f.u. in the oral cavity. In conclusion, the alleviation of oral candidiasis by LF feeding in this model may correlate with the enhancement of the number of leukocytes and their cytokine responses in regional lymph nodes against infection.

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2004-06-01
2024-12-08
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References

  1. Abe S., Okutomi T., Tansho S., Ishibashi H., Wakabayashi H., Teraguchi S., Hayasawa H., Yamaguchi H. 2000; Augmentation by lactoferrin of host defense against Candida infection in mice. In Lactoferrin: Structure . Function and Applications pp 195–201 Edited by Shimazaki K., Tsuda H., Tomita M., Kuwata T., Perraudin J.-P. Amsterdam: Elsevier;
    [Google Scholar]
  2. Akagawa G., Abe S., Yamaguchi H. 1995; Mortality of Candida albicans -infected mice is facilitated by superinfection of Escherichia coli or administration of its lipopolysaccharide. J Infect Dis 171:1539–1544 [CrossRef]
    [Google Scholar]
  3. Artym J., Zimecki M., Kruzel M. L. 2003; Reconstitution of the cellular immune response by lactoferrin in cyclophosphamide-treated mice is correlated with renewal of T cell compartment. Immunobiology 207:197–205 [CrossRef]
    [Google Scholar]
  4. Bhimani R. S., Vendrov Y., Furmanski P. 1999; Influence of lactoferrin feeding and injection against systemic staphylococcal infections in mice. J Appl Microbiol 86:135–144 [CrossRef]
    [Google Scholar]
  5. Brock J. 1995; Lactoferrin: a multifunctional immunoregulatory protein?. Immunol Today 16:417–419 [CrossRef]
    [Google Scholar]
  6. Elahi S., Pang G., Clancy R., Ashman R. B. 2000; Cellular and cytokine correlates of mucosal protection in murine model of oral candidiasis. Infect Immun 68:5771–5777 [CrossRef]
    [Google Scholar]
  7. Farah C. S., Elahi S., Pang G., Gotjamanos T., Seymour G. J., Clancy R. L., Ashman R. B. 2001; T cells augment monocyte and neutrophil function in host resistance against oropharyngeal candidiasis. Infect Immun 69:6110–6118 [CrossRef]
    [Google Scholar]
  8. Farah C. S., Gotjamanos T., Seymour G. J., Ashman R. B. 2002; Cytokines in the oral mucosa of mice infected with Candida albicans . Oral Microbiol Immunol 17:375–378 [CrossRef]
    [Google Scholar]
  9. Fidel P. L. Jr 2002; Immunity to Candida . Oral Dis 8 (Suppl. 2):69–75
    [Google Scholar]
  10. Haversen L. A., Engberg I., Baltzer L., Dolphin G., Hanson L. A., Mattsby-Baltzer I. 2000; Human lactoferrin and peptides derived from a surface-exposed helical region reduce experimental Escherichia coli urinary tract infection in mice. Infect Immun 68:5816–5823 [CrossRef]
    [Google Scholar]
  11. Hermann P., Berek Z., Nagy G., Kamotsay K., Rozgonyi F. 2001; Pathogenesis, microbiological and clinical aspects of oral candidiasis (candidosis). Acta Microbiol Immunol Hung 48:479–495 [CrossRef]
    [Google Scholar]
  12. Ishii K., Takamura N., Shinohara M., Wakui N., Shin H., Sumino Y., Ohmoto Y., Teraguchi S., Yamauchi K. 2003; Long-term follow-up of chronic hepatitis C patients treated with oral lactoferrin for 12 months. Hepatol Res 25:226–233 [CrossRef]
    [Google Scholar]
  13. Iwasa M., Kaito M., Ikoma J., Takeo M., Imoto I., Adachi Y., Yamauchi K., Koizumi R., Teraguchi S. 2002; Lactoferrin inhibits hepatitis C virus viremia in chronic hepatitis C patients with high viral loads and HCV genotype 1b. Am J Gastroenterol 97:766–767 [CrossRef]
    [Google Scholar]
  14. Kuhara T., Iigo M., Itoh T., Ushida Y., Sekine K., Terada N., Okamura H., Tsuda H. 2000; Orally administered lactoferrin exerts an antimetastatic effect and enhances production of IL-18 in the intestinal epithelium. Nutr Cancer 38:192–199 [CrossRef]
    [Google Scholar]
  15. Kuipers M. E., de Vries H. G., Eikelboom M. C., Meijer D. K., Swart P. J. 1999; Synergistic fungistatic effects of lactoferrin in combination with antifungal drugs against clinical Candida isolates. Antimicrob Agents Chemother 43:2635–2641
    [Google Scholar]
  16. Lopez-Ribot J. L., McAtee R. K., Perea S., Kirkpatrick W. R., Rinaldi M. G., Patterson T. F. 1999; Multiple resistant phenotypes of Candida albicans coexist during episodes of oropharyngeal candidiasis in human immunodeficiency virus-infected patients. Antimicrob Agents Chemother 43:1621–1630
    [Google Scholar]
  17. Maebashi K., Itoyama T., Uchida K., Suegara N., Yamaguchi H. 1994; A novel model of cutaneous candidiasis produced in prednisolone-treated guinea-pigs. J Med Vet Mycol 32:349–359 [CrossRef]
    [Google Scholar]
  18. Masci J. R. 2000; Complete response of severe, refractory oral candidiasis to mouthwash containing lactoferrin and lysozyme. AIDS 14:2403–2404 [CrossRef]
    [Google Scholar]
  19. Sekine K., Ushida Y., Kuhara T. & 8 other authors; 1997; Inhibition of initiation and early stage development of aberrant crypt foci and enhanced natural killer activity in male rats administered bovine lactoferrin concomitantly with azoxymethane. Cancer Lett 121:211–216 [CrossRef]
    [Google Scholar]
  20. Takakura N., Sato Y., Ishibashi H., Oshima H., Uchida K., Yamaguchi H., Abe S. 2003a; A novel murine model of oral candidiasis with local symptoms characteristic of oral thrush. Microbiol Immunol 47:321–326 [CrossRef]
    [Google Scholar]
  21. Takakura N., Wakabayashi H., Ishibashi H., Teraguchi S., Tamura Y., Yamaguchi H., Abe S. 2003b; Oral lactoferrin treatment of experimental oral candidiasis in mice. Antimicrob Agents Chemother 47:2619–2623 [CrossRef]
    [Google Scholar]
  22. Tansho S., Abe S., Yamaguchi H. 1994; Inhibition of Candida albicans growth by murine peritoneal neutrophils and augmentation of the inhibitory activity by bacterial lipopolysaccharide and cytokines. Microbiol Immunol 38:379–383 [CrossRef]
    [Google Scholar]
  23. Teraguchi S., Shin K., Ozawa K., Nakamura S., Fukuwatari Y., Tsuyuki S., Namihira H., Shimamura S. 1995; Bacteriostatic effect of orally administered bovine lactoferrin on proliferation of Clostridium species in the gut of mice fed bovine milk. Appl Environ Microbiol 61:501–506
    [Google Scholar]
  24. Tomita M., Wakabayashi H., Yamauchi K., Teraguchi S., Hayasawa H. 2002; Bovine lactoferrin and lactoferricin derived from milk: production and applications. Biochem Cell Biol 80:109–112 [CrossRef]
    [Google Scholar]
  25. Trinchieri G., Kubin M., Bellone G., Cassatella M. A. 1993; Cytokine cross-talk between phagocytic cells and lymphocytes: relevance for differentiation/activation of phagocytic cells and regulation of adaptive immunity. J Cell Biochem 53:301–308 [CrossRef]
    [Google Scholar]
  26. Vorland L. H. 1999; Lactoferrin: a multifunctional glycoprotein. APMIS 107:971–981 [CrossRef]
    [Google Scholar]
  27. Wada T., Aiba Y., Shimizu K., Takagi A., Miwa T., Koga Y. 1999; The therapeutic effect of bovine lactoferrin in the host infected with Helicobacter pylori . Scand J Gastroenterol 34:238–243 [CrossRef]
    [Google Scholar]
  28. Wakabayashi H., Abe S., Okutomi T., Tansho S., Kawase K., Yamaguchi H. 1996; Cooperative anti- Candida effects of lactoferrin or its peptides in combination with azole antifungal agents. Microbiol Immunol 40:821–825 [CrossRef]
    [Google Scholar]
  29. Wakabayashi H., Abe S., Teraguchi S., Hayasawa H., Yamaguchi H. 1998; Inhibition of hyphal growth of azole-resistant strains of Candida albicans by triazole antifungal agents in the presence of lactoferrin-related compounds. Antimicrob Agents Chemother 42:1587–1591
    [Google Scholar]
  30. Wakabayashi H., Uchida K., Yamauchi K., Teraguchi S., Hayasawa H., Yamaguchi H. 2000; Lactoferrin given in food facilitates dermatophytosis cure in guinea pig models. J Antimicrob Chemother 46:595–602 [CrossRef]
    [Google Scholar]
  31. Wakabayashi H., Takakura N., Yamauchi K., Teraguchi S., Uchida K., Yamaguchi H., Tamura Y. 2002; Effect of lactoferrin feeding on the host antifungal response in guinea-pigs infected or immunised with Trichophyton mentagrophytes . J Med Microbiol 51:844–850
    [Google Scholar]
  32. Wakabayashi H., Takakura N., Teraguchi S., Tamura Y. 2003; Lactoferrin feeding augments peritoneal macrophage activities in mice intraperitoneally injected with inactivated Candida albicans . Microbiol Immunol 47:37–43 [CrossRef]
    [Google Scholar]
  33. Walsh T. J., Gonzalez C. E., Piscitelli S. & 7 other authors; 2000; Correlation between in vitro and in vivo antifungal activities in experimental fluconazole-resistant oropharyngeal and esophageal candidiasis. J Clin Microbiol 38:2369–2373
    [Google Scholar]
  34. Yamauchi K., Hiruma M., Yamazaki N., Wakabayashi H., Kuwata H., Teraguchi S., Hayasawa H., Suegara N., Yamaguchi H. 2000; Oral administration of bovine lactoferrin for treatment of tinea pedis.A placebo-controlled, double-blind study. Mycoses 43:197–202 [CrossRef]
    [Google Scholar]
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