1887

Abstract

Fifty-one consecutive isolates of , collected during a 2-year period in the north-east of Italy, were subjected to IS-RFLP analysis to detect the presence of clusters and assigned to one of the three genotypic groups delineated by single nucleotide polymorphisms in the genes and . All the isolates collected from the local population belonged to group 2 or 3, while group 1 isolates were found only in specimens collected from African immigrants. Clustered cases of tuberculosis, which are likely to be related to recently transmitted infection, were found to be significantly associated with group 2. In the local situation, strains belonging to this group may therefore present a higher risk of transmission.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05471-0
2004-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/53/2/JM530213.html?itemId=/content/journal/jmm/10.1099/jmm.0.05471-0&mimeType=html&fmt=ahah

References

  1. Bifani P., Moghazeh S., Shopsin B., Driscoll J., Ravikovitch A., Kreiswirth B. N. 2000; Molecular characterization of Mycobacterium tuberculosis H37Rv/Ra variants: distinguishing the mycobacterial laboratory strain. J Clin Microbiol 38:3200–3204
    [Google Scholar]
  2. Bifani P. J., Mathema B., Kurepina N. E., Kreiswirth B. N. 2002; Global dissemination of the Mycobacterium tuberculosis W-Beijing family strains. Trends Microbiol 10:45–52 [CrossRef]
    [Google Scholar]
  3. Dice L. R. 1945; Measures of amount of ecological association between species. Ecology 26:297–302 [CrossRef]
    [Google Scholar]
  4. Friedman C. R., Stoeckle M. Y., Johnson W. D. Jr, Riley L. W. 1995; Double-repetitive-element PCR method for subtyping Mycobacterium tuberculosis clinical isolates. J Clin Microbiol 33:1383–1384
    [Google Scholar]
  5. Lion T., Haas O. A. 1990; Nonradioactive labeling of probe with digoxigenin by polymerase chain reaction. Anal Biochem 188:335–337 [CrossRef]
    [Google Scholar]
  6. Moro M. L., Salamina G., Gori A., Penati V., Sacchetti R., Mezzetti F., Infuso A., Sodano L. 2002; Two-year population-based molecular epidemiological study of tuberculosis transmission in the metropolitan area of Milan, Italy. Eur J Clin Microbiol Infect Dis 21:114–122 [CrossRef]
    [Google Scholar]
  7. Nastasi A., Mammina C. 1999; Epidemiological study of tuberculosis in Palermo, Italy: IS 6110 fingerprinting of Mycobacterium tuberculosis strains isolated in the years 1994–1998. Infection 27:318–322 [CrossRef]
    [Google Scholar]
  8. Rhee J. T., Piatek A. S., Small P. M., Harris L. M., Chaparro S. V., Kramer F. R., Alland D. 1999; Molecular epidemiologic evaluation of transmissibility and virulence of Mycobacterium tuberculosis . J Clin Microbiol 37:1764–1770
    [Google Scholar]
  9. Small P. M., Hopewell P. C., Singh S. P., Paz A., Parsonnet J., Ruston D. C., Schecter G. F., Daley C. L., Schoolnik G. K. 1994; The epidemiology of tuberculosis in San Francisco.A population-based study using conventional and molecular methods. N Engl J Med 330:1703–1709 [CrossRef]
    [Google Scholar]
  10. Sreevatsan S., Pan X., Stockbauer K. E., Connell N. D., Kreiswirth B. N., Whittam T. S., Musser J. M. 1997; Restricted structural gene polymorphism in the Mycobacterium tuberculosis complex indicates evolutionarily recent global dissemination. Proc Natl Acad Sci U S A 94:9869–9874 [CrossRef]
    [Google Scholar]
  11. van Embden J. D. A., Cave M. D., Crawford J. T. & 8 other authors; 1993; Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 31:406–409
    [Google Scholar]
  12. van Soolingen D. 2001; Molecular epidemiology of tuberculosis and other mycobacterial infections: main methodologies and achievements. J Intern Med 249:1–26
    [Google Scholar]
  13. Vynnycky E., Nagelkerke N., Borgdorff M. W., van Soolingen D., van Embden J. D. A., Fine P. E. M. 2001; The effect of age and study duration on the relationship between 'clustering’ of DNA fingerprint patterns and the proportion of tuberculosis disease attributable to recent transmission. Epidemiol Infect 126:43–62
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05471-0
Loading
/content/journal/jmm/10.1099/jmm.0.05471-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error