1887

Journal of Medical Microbiology logo

Volume 53, Issue 6

ELISA for early diagnosis of histoplasmosis Free

Abstract

An ELISA was developed and evaluated as a method for detecting antibodies against glycosylated and deglycosylated histoplasmin (HMIN). Sera from patients with histoplasmosis, paracoccidioidomycosis, sporotrichosis, coccidioidomycosis, aspergillosis, cryptococcosis and healthy donors were tested by ELISA against purified, deglycosylated histoplasmin (ptHMIN) and compared with purified, native (i.e. glycosylated) histoplasmin (pHMIN). Although cross-reactivity was not abolished when ptHMIN was used in the test, it was reduced (pHMIN ELISA 93 % versus ptHMIN ELISA 96 %). However, there were statistically significant differences between the sensitivities of these two methods for the detection of antibodies (pHMIN ELISA 57 % versus ptHMIN ELISA 92 %; < 0.001) and between the efficiency of the methods (pHMIN ELISA 83 % versus ptHMIN ELISA 95 %; < 0.001). These parameters compare better than previously published data relating to the use of treated HMIN in diagnostic ELISAs. Some of the reactivities of serum samples were compared by immunoblotting using deglycosylated HMIN and by immunodiffusion using the crude antigen. The results demonstrated that cross-reactions with heterologous sera in both ELISAs could also be observed in immunoblotting and arose from shared protein epitopes. These data suggest that ELISA using deglycosylated HMIN is a very sensitive diagnostic method and, by using commercially available antigen, it can be easily standardized and performed faster than previous Western blot-based tests using the same antigen. It provides a useful adjunct to existing methods of diagnosis that could be applied even in situations where laboratory facilities were relatively limited.

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05469-0
2004-06-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/53/6/JM530607.html?itemId=/content/journal/jmm/10.1099/jmm.0.05469-0&mimeType=html&fmt=ahah

References

  1. Bradsher R. W. 1996; Histoplasmosis and blastomycosis. Clin Infect Dis 22:S102–S111 [CrossRef]
     [Google Scholar]
  2. Brock E. G., Reiss E., Pine L., Kaufman L. 1983; Effect of periodate oxidation on the detection of antibodies against M antigen of histoplasmin by enzyme immunoassay (EIA) inhibition. Curr Microbiol 10:177–180
     [Google Scholar]
  3. Bullock W. E. 1995; Histoplasma capsulatum . In Principles and Practice of Infectious Diseases . pp 2340–2353 Edited by Mandell G. L., Bennett J., Dolin R. New York: Churchill Livingstone;
  4. Dijkstra J. W. 1989; Histoplasmosis. Dermatol Clin 7:251–258
     [Google Scholar]
  5. Gomez B. L., Figueroa J. I., Hamilton A. J., Ortiz B. L., Robledo M. A., Restrepo A., Hay R. J. 1997; Development of a novel antigen detection test for histoplasmosis. J Clin Microbiol 35:2618–2622
     [Google Scholar]
  6. Goodwin R. A. Jr, Shapiro J. L., Thurman G. H., Thurman S. S., Des Prez R. M. 1980; Disseminated histoplasmosis: clinical and pathologic correlations. Medicine (Baltimore) 59:1–33
     [Google Scholar]
  7. Kaufman L., Kovacs J. A., Reiss E. 1997; Clinical immunomycology. In Manual of Clinical Laboratory Immunology . , 5th edn. pp 559–576 Edited by Rose N. R., Conway de Macario E., Folds J. D., Lane H. C., Nakamura R. M. Washington, DC: American Society for Microbiology;
  8. Kostiala A. A., Kostiala I. 1981; Enzyme-linked immunosorbent assay (ELISA) for IgM, IgG and IgA class antibodies against Candida albicans antigens: development and comparison with other methods. Sabouraudia 19:123–134 [CrossRef]
     [Google Scholar]
  9. Kumar B. V., Medoff G., Kobayashi G. S., Sieling W. L. 1985; Cross-reacting human and rabbit antibodies to antigens of Histoplasma capsulatum , Candida albicans , and Saccharomyces cerevisiae . Infect Immun 48:806–812
     [Google Scholar]
  10. Kwon-Chung K. J., Bennet J. E. 1992 Medical Mycology , 2nd edn. Malvern, PA: Lea & Febiger;
     [Google Scholar]
  11. Maiga Y. I., Marjolet M. 1985; Value and limits of serological methods: electrosyneresis and ELISA for the study of the prevalence of histoplasmosis in Mali. Bull Soc Pathol Exot Filiales 78:585–593 (in French
    [Google Scholar]
  12. McKinsey D. S., Spiegel R. A., Hutwagner L. & 7 other authors; 1997; Prospective study of histoplasmosis in patients infected with human immunodeficiency virus: incidence, risk factors, and pathophysiology. Clin Infect Dis 24:1195–1203 [CrossRef]
     [Google Scholar]
  13. Palmer D. F., Kaufman L., Kaplan W., Caballero J. 1977; The complement fixation test. In Serodiagnosis of Mycotic Diseases pp 155–178 Edited by Balows A. Springfield, IL: Charles C. Thomas;
     [Google Scholar]
  14. Pizzini C. V., Zancopé-Oliveira R. M., Reiss E., Hajjeh R., Kaufman L., Peralta J. M. 1999; Evaluation of a Western blot test in an outbreak of acute pulmonary histoplasmosis. Clin Diagn Lab Immunol 6:20–23
     [Google Scholar]
  15. Raman C., Khardori N., Von Behren L. A., Wheat L. J., Tewari R. P. 1990; Evaluation of an ELISA for the detection of anti- Histoplasma ribosomal and antihistoplasmin antibodies in histoplasmosis. J Clin Lab Anal 4:199–207 [CrossRef]
     [Google Scholar]
  16. Rios-Fabra A., Moreno A. R., Isturiz R. 1994; Fungal infection in Latin American countries. Infect Dis Clin North Am 8:129–154
     [Google Scholar]
  17. Standard P. G., Kaufman L. 1976; Specific immunological test for the rapid identification of members of the genus Histoplasma . J Clin Microbiol 3:191–199
     [Google Scholar]
  18. Torres M., Diaz H., Herrera T., Sada E. 1993; Evaluation of enzyme linked immunosorbent-assay and Western blot for diagnosis of histoplasmosis. Rev Invest Clin 45:155–160
     [Google Scholar]
  19. Wheat L. J. 1996; Histoplasmosis in the acquired immunodeficiency syndrome. Curr Top Med Mycol 7:7–18
     [Google Scholar]
  20. Wheat L. J. 2001; Laboratory diagnosis of histoplasmosis: update 2000. Semin Respir Infect 16:131–140 [CrossRef]
     [Google Scholar]
  21. Wheat L. J., Kauffman C. A. 2003; Histoplasmosis. Infect Dis Clin North Am 17:1–19 [CrossRef]
     [Google Scholar]
  22. Wheat L. J., Connolly-Stringfield P., Blair R., Connolly K., Garringer T., Katz B. P. 1991; Histoplasmosis relapse in patients with AIDS: detection using Histoplasma capsulatum variety capsulatum antigen levels. Ann Intern Med 115:936–941 [CrossRef]
     [Google Scholar]
  23. Wheat L. J., Wheat H., Connolly P., Kleiman M., Supparatpinyo K. S., Nelson K., Bradsher R., Restrepo A. 1997; Cross-reactivity in Histoplasma capsulatum var. capsulatum antigen assays of urine samples from patients with endemic mycoses. Clin Infect Dis 24:1169–1171 [CrossRef]
     [Google Scholar]
  24. Wheat L. J., Garringer T., Brizendine E., Conolly P. 2002; Diagnosis of histoplasmosis by antigen detection based upon experience at the histoplasmosis reference laboratory. Diagn Microbiol Infect Dis 43:29–37 [CrossRef]
     [Google Scholar]
  25. Zancopé-Oliveira R. M., Bragg S. L., Hurst S. F., Peralta J. M., Reiss E. 1993; Evaluation of cation exchange chromatography for the isolation of M glycoprotein from histoplasmin. J Med Vet Mycol 31:29–41 [CrossRef]
     [Google Scholar]
  26. Zancopé-Oliveira R. M., Bragg S. L., Reiss E., Wanke B., Peralta J. M. 1994a; Effects of histoplasmin M antigen chemical and enzymatic deglycosylation on cross-reactivity in the enzyme-linked immunoelectrotransfer blot method. Clin Diag Lab Immun 1:390–393
     [Google Scholar]
  27. Zancopé-Oliveira R. M., Bragg S. L., Reiss E., Peralta J. M. 1994b; Immunochemical analysis of the H and M glycoproteins from Histoplasma capsulatum . Clin Diagn Lab Immun 1:563–568
     [Google Scholar]
  28. Zancopé-Oliveira R. M., Reiss E., Lott T. J., Mayer L. W., Deepe G. S. Jr 1999; Molecular cloning, characterization, and expression of the M antigen of Histoplasma capsulatum . Infect Immun 67:1947–1953
     [Google Scholar]
  29. Zimmerman S. E., French M. L., Kleiman M. B., Wheat L. J. 1990; Evaluation of an enzyme-linked immunosorbent assay that uses ferrous metal beads for determination of antihistoplasmal immunoglobulins G and M. J Clin Microbiol 28:59–64
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05469-0
Loading
ELISA for early diagnosis of histoplasmosis
J. Med. Microbiol. 53, 509 (2004); https://doi.org/10.1099/jmm.0.05469-0
/content/journal/jmm/10.1099/jmm.0.05469-0
/content/journal/jmm/10.1099/jmm.0.05469-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error