1887

Abstract

The BD ProbeTec ET system for identification of complex (MTBC) isolates from BACTEC 12B culture vials was evaluated in comparison with BACTEC NAP (-nitro-α-acetylamino-β-hydroxy-propiophenone) differentiation. Of 145 mycobacterial isolates tested, comprising 89 MTBC and 56 non-tuberculous mycobacteria (NTM), BD ProbeTec ET correctly identified 87 MTBC and 56 NTM but missed two MTBC. Three NTM were misidentified when NAP was incubated at 37 °C only. Overall sensitivity, specificity and positive and negative predictive values were respectively 97.8, 100, 100 and 96.6 % for the BD ProbeTec ET system and 100, 94.6, 96.7 and 94.6 % for NAP.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05454-0
2004-01-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jmm/53/1/JM530109.html?itemId=/content/journal/jmm/10.1099/jmm.0.05454-0&mimeType=html&fmt=ahah

References

  1. American Thoracic Society. 1997; Rapid diagnostic tests for tuberculosis: what is the appropriate use?. Am J Respir Crit Care Med 155:1804–1814 [CrossRef]
    [Google Scholar]
  2. Bergmann J. S., Woods G. L. 1998; Clinical evaluation of the BDProbeTec strand displacement amplification assay for rapid diagnosis of tuberculosis. J Clin Microbiol 36:2766–2768
    [Google Scholar]
  3. Bergmann J. S., Woods G. L. 1999; Enhanced amplified Mycobacterium tuberculosis direct test for detection of Mycobacterium tuberculosis complex in positive BACTEC 12B broth cultures of respiratory specimens. J Clin Microbiol 37:2099–2101
    [Google Scholar]
  4. Bergmann J. S., Keating W. E., Woods G. L. 2000; Clinical evaluation of the BDProbeTec ET system for rapid detection of Mycobacterium tuberculosis . J Clin Microbiol 38:863–865
    [Google Scholar]
  5. Kent P. T., Kubica G. P. 1985 Public Health Mycobacteriology: a Guide for the Level III Laboratory Atlanta, GA: Centers for Disease Control, US Department of Health and Human Services;
    [Google Scholar]
  6. Little M. C., Andrews J., Moore R. & 22 other authors; 1999; Strand displacement amplification and homogeneous real-time detection incorporated in a second-generation DNA probe system, BDProbeTec ET. Clin Chem 45:777–784
    [Google Scholar]
  7. Mazzarelli G., Rindi L., Piccoli P., Scarparo C., Garzelli C., Tortoli E. 2003; Evaluation of the BDProbeTec ET system for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary samples: a multicenter study. J Clin Microbiol 41:1779–1782 [CrossRef]
    [Google Scholar]
  8. Petroff S. A. 1915; A new and rapid method for the isolation and cultivation of tubercle bacilli directly from the sputum and feces. J Exp Med 21:38–42 [CrossRef]
    [Google Scholar]
  9. Piersimoni C., Scarparo C., Piccoli P., Rigon A., Ruggiero G., Nista D., Bornigia S. 2002; Performance assessment of two commercial amplification assays for direct detection of Mycobacterium tuberculosis complex from respiratory and extrapulmonary specimens. J Clin Microbiol 40:4138–4142 [CrossRef]
    [Google Scholar]
  10. Reisner B. S., Gatson A. M., Woods G. L. 1994; Use of Gen-Probe AccuProbes to identify Mycobacterium avium complex, Mycobacterium tuberculosis complex, Mycobacterium kansasii , and Mycobacterium gordonae directly from BACTEC TB broth cultures. J Clin Microbiol 32:2995–2998
    [Google Scholar]
  11. Siddiqi S. H. 1995 BACTEC460 TB System, Products and Procedure Manual , revision D Sparks, MD: Becton Dickinson;
    [Google Scholar]
  12. Smith M. B., Bergmann J. S., Woods G. L. 1997; Detection of Mycobacterium tuberculosis in BACTEC 12B broth cultures by the Roche Amplicor PCR assay. J Clin Microbiol 35:900–902
    [Google Scholar]
  13. Spargo C. A., Fraiser M. S., Van Cleve M., Wright D. J., Nycz C. M., Spears P. A., Walker G. T. 1996; Detection of M.tuberculosis DNA using thermophilic strand displacement amplification. Mol Cell Probes 10:247–256 [CrossRef]
    [Google Scholar]
  14. Tortoli E., Lavinia F., Simonetti M. T. 1998; Early detection of Mycobacterium tuberculosis in BACTEC cultures by ligase chain reaction. J Clin Microbiol 36:2791–2792
    [Google Scholar]
  15. WHO 2003 WHO report 2003 Global tuberculosis control Geneva: World Health Organization; http://www.who.int/gtb/publications/ globrep/index.html
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05454-0
Loading
/content/journal/jmm/10.1099/jmm.0.05454-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error