1887

Abstract

The BD ProbeTec ET system for identification of complex (MTBC) isolates from BACTEC 12B culture vials was evaluated in comparison with BACTEC NAP (-nitro-α-acetylamino-β-hydroxy-propiophenone) differentiation. Of 145 mycobacterial isolates tested, comprising 89 MTBC and 56 non-tuberculous mycobacteria (NTM), BD ProbeTec ET correctly identified 87 MTBC and 56 NTM but missed two MTBC. Three NTM were misidentified when NAP was incubated at 37 °C only. Overall sensitivity, specificity and positive and negative predictive values were respectively 97.8, 100, 100 and 96.6 % for the BD ProbeTec ET system and 100, 94.6, 96.7 and 94.6 % for NAP.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05454-0
2004-01-01
2019-11-15
Loading full text...

Full text loading...

/deliver/fulltext/jmm/53/1/JM530109.html?itemId=/content/journal/jmm/10.1099/jmm.0.05454-0&mimeType=html&fmt=ahah

References

  1. American Thoracic Society ( 1997;). Rapid diagnostic tests for tuberculosis: what is the appropriate use? Am J Respir Crit Care Med 155, 1804–1814.[CrossRef]
    [Google Scholar]
  2. Bergmann, J. S. & Woods, G. L. ( 1998;). Clinical evaluation of the BDProbeTec strand displacement amplification assay for rapid diagnosis of tuberculosis. J Clin Microbiol 36, 2766–2768.
    [Google Scholar]
  3. Bergmann, J. S. & Woods, G. L. ( 1999;). Enhanced amplified Mycobacterium tuberculosis direct test for detection of Mycobacterium tuberculosis complex in positive BACTEC 12B broth cultures of respiratory specimens. J Clin Microbiol 37, 2099–2101.
    [Google Scholar]
  4. Bergmann, J. S., Keating, W. E. & Woods, G. L. ( 2000;). Clinical evaluation of the BDProbeTec ET system for rapid detection of Mycobacterium tuberculosis. J Clin Microbiol 38, 863–865.
    [Google Scholar]
  5. Kent, P. T. & Kubica, G. P. ( 1985;). Public Health Mycobacteriology: a Guide for the Level III Laboratory. Atlanta, GA: Centers for Disease Control, US Department of Health and Human Services.
  6. Little, M. C., Andrews, J., Moore, R. & 22 other authors ( 1999;). Strand displacement amplification and homogeneous real-time detection incorporated in a second-generation DNA probe system, BDProbeTec ET. Clin Chem 45, 777–784.
    [Google Scholar]
  7. Mazzarelli, G., Rindi, L., Piccoli, P., Scarparo, C., Garzelli, C. & Tortoli, E. ( 2003;). Evaluation of the BDProbeTec ET system for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary samples: a multicenter study. J Clin Microbiol 41, 1779–1782.[CrossRef]
    [Google Scholar]
  8. Petroff, S. A. ( 1915;). A new and rapid method for the isolation and cultivation of tubercle bacilli directly from the sputum and feces. J Exp Med 21, 38–42.[CrossRef]
    [Google Scholar]
  9. Piersimoni, C., Scarparo, C., Piccoli, P., Rigon, A., Ruggiero, G., Nista, D. & Bornigia, S. ( 2002;). Performance assessment of two commercial amplification assays for direct detection of Mycobacterium tuberculosis complex from respiratory and extrapulmonary specimens. J Clin Microbiol 40, 4138–4142.[CrossRef]
    [Google Scholar]
  10. Reisner, B. S., Gatson, A. M. & Woods, G. L. ( 1994;). Use of Gen-Probe AccuProbes to identify Mycobacterium avium complex, Mycobacterium tuberculosis complex, Mycobacterium kansasii, and Mycobacterium gordonae directly from BACTEC TB broth cultures. J Clin Microbiol 32, 2995–2998.
    [Google Scholar]
  11. Siddiqi, S. H. ( 1995;). BACTEC460 TB System, Products and Procedure Manual, revision D. Sparks, MD: Becton Dickinson.
  12. Smith, M. B., Bergmann, J. S. & & Woods, G. L. ( 1997;). Detection of Mycobacterium tuberculosis in BACTEC 12B broth cultures by the Roche Amplicor PCR assay. J Clin Microbiol 35, 900–902.
    [Google Scholar]
  13. Spargo, C. A., Fraiser, M. S., Van Cleve, M., Wright, D. J., Nycz, C. M., Spears, P. A. & Walker, G. T. ( 1996;). Detection of M.tuberculosis DNA using thermophilic strand displacement amplification. Mol Cell Probes 10, 247–256.[CrossRef]
    [Google Scholar]
  14. Tortoli, E., Lavinia, F. & Simonetti, M. T. ( 1998;). Early detection of Mycobacterium tuberculosis in BACTEC cultures by ligase chain reaction. J Clin Microbiol 36, 2791–2792.
    [Google Scholar]
  15. WHO ( 2003;). WHO report 2003. Global tuberculosis control. Geneva: World Health Organization. http://www.who.int/gtb/publications/ globrep/index.html
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05454-0
Loading
/content/journal/jmm/10.1099/jmm.0.05454-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error