1887

Abstract

Strains of , a periodontopathic bacterium, are classified into six genotypic variants based on nucleotide sequence differences in the gene encoding FimA. A PCR assay using primer sets specific for each genotype has demonstrated that the most predominant genotype in periodontitis patients is type II, which is now commonly referred to as the periodontitis-associated genotype of . However, the potential for false type II positives caused by cross-hybridization of type II -specific primers with type Ib has complicated the genotyping. A previous study developed new primers that specifically amplified only the DNA fragment of type II . The aim of the present study was to assess the prevalence of genotypes in Korean adults and to reconfirm the relationship between type II and periodontitis using the new primers. Among 412 Korean adults, was detected in 97.5 % of patients and 57.8 % of healthy subjects. Type II was the most widely distributed type among healthy and periodontitis subjects. Organisms with types II, Ib and IV had a significant frequency of occurrence in periodontitis subjects. Statistical analysis, however, revealed that a more significant correlation was found between periodontitis and the occurrence of type Ib .

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2013-09-01
2020-01-22
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