1887

Abstract

A murine model was used to evaluate the role of anti-CD3 in modulating a Th1-type response by restimulation of T-cells after immunization with the 57 kDa immunodominant antigen of 1 outer-membrane proteins (OMPs), followed by infection after immunization. To observe the effect of anti-CD3, other T-cell cultures were also established following anti-CD1, anti-IL2 and phytohaemagglutinin stimulation. Anti-CD3 stimulation of reconstituted T-cells showed ‘mean’ levels of CD4 and CD25 were enhanced by 34.5 and 31.1 % in immunized mice, which was comparable to 53.2 and 50.7 %, respectively, in challenged-immunized mice, and were dominant over CD8 T-cells. Levels of IL2 generated by anti-CD3-stimulated T-cells of immunized mice were greater than those of unstimulated T-cells and were significantly elevated in challenged-immunized mice. The reactivity of T-cells indicated their complete responsiveness, as anti-CD3 antibody might not inhibit the migration of the macrophages but rather inhibit IL4. These macrophage factors synergistically act with anions towards an activated response, which in turn provokes IL2 secretion with a low degree of internalization of its receptor. Thus, sharing of IL2 to form a high-affinity receptor complex with CD4 T-cells through motive signals suggested a generalized T-cell activation with increased humoral responses. Macrophage migration inhibition factor (MIF) and IL4 responses during anti-CD3 stimulation of immunized mice indicated that the role of anti-CD3 in generation of O is due to a synergistic effect by Th1 subsets of Th0 cells. The above findings should have implications for understanding the immunoregulatory role of anti-CD3 associated with 57 kDa antigen in immunoprophylactic measures.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05420-0
2004-11-01
2019-11-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/53/11/JM531104.html?itemId=/content/journal/jmm/10.1099/jmm.0.05420-0&mimeType=html&fmt=ahah

References

  1. Acha-Orbea, H. ( 1993;). Role of super antigens in microbial infections? Res Immunol 144, 198–201.[CrossRef]
    [Google Scholar]
  2. Akaike, T. ( 2001;). Role of free radicals in viral pathogenesis and mutation. Rev Med Virol 11, 87–101.[CrossRef]
    [Google Scholar]
  3. Azim, T., Sarker, M. S., Hamadani, J., Khanum, N., Halder, R. C., Salam, M. A. & Albert, M. J. ( 1996;). Alterations in lymphocyte phenotype and function in children with Shigellosis who develop complications. Clin Diagn Lab Immunol 3, 191–196.
    [Google Scholar]
  4. Bhattacharya, S. K., Sinha, A. K., Sen, D., Sengupta, P. G., Lall, R. & Pal, S. C. ( 1988;). Extraintestinal manifestation of Shigellosis during epidemic of bacillary dysenteriae in Port Blair, Andaman & Nicobar Island (India). J Assoc Physicians India 36, 319–320.
    [Google Scholar]
  5. Bloom, B. & David, J. ( 1973;). In In Vitro Methods in Cell Mediated and Tumor Immunity, p. 122. New York: Academic Press.
  6. Borisova, E. V. ( 1999;). The role of the structural components of bacterial lipopolysaccharide in its inductive immunosuppressive activity. Mikrobiol Z 61, 36–41 (in Russian).
    [Google Scholar]
  7. Brams, P. & Claesson, M. H. ( 1989;). T-cell activation.I. Evidence for a functional linkage between class I MHC antigens and the Tc-Ti complex. Immunology 66, 348–353.
    [Google Scholar]
  8. Cantrell, D., Davies, A. A., Londei, M., Feldman, M. & Crumpton, M. J. ( 1987;). Association of phosphorylation of the T3 antigen with immune activation of T-lymphocytes. Nature 325, 540. 540.[CrossRef]
    [Google Scholar]
  9. Chakraborti, S. & Sinha, A. K. ( 1994;). A major antigenic outer membrane protein of Shigella dysenteriae 1: role in adhesion to cultured mammalian cells. Med Sci Res 22, 835–836.
    [Google Scholar]
  10. Chakraborti, S. & Sinha, A. K. ( 1997;). Release of interleukin-2 induced by major antigenic outer membrane protein of Shigella dysenteriae type 1 in natural infection. Microbios 92, 123–132.
    [Google Scholar]
  11. Clausen, J. E. ( 1971;). Tuberculin induced migration inhibition of human peripheral leucocytes in agarose medium. Acta Allergol 26, 56–80.[CrossRef]
    [Google Scholar]
  12. Clement, L. T. ( 1992;). Isoform of the common CD45 leukocyte antigen family: markers for human T-cell differentiation. J Clin Immunol 12, 1–5.[CrossRef]
    [Google Scholar]
  13. Czarny, A., Witkowska, D. & Mulczyk, M. ( 1992;). Effect of outer membrane proteins from Shigella on humoral immunity induced in mice by SRBC. Arch Immunol Ther Exp (Warsz) 40, 129–134.
    [Google Scholar]
  14. Delves, P. J. ( 1994;). In Cellular Immunology (Labfax), p. 230. Edited by B. D. Hames and D. Rickwood. Blackwell Scientific Publications: Oxford, UK.
  15. Fang, S.-H., Hwang, L.-H., Chen, D.-S. & Chiang, B.-L. ( 2000;). Ribavirin enhancement of hepatitis C virus core antigen-specific type 1 T helper cell responses correlates with the increased IL-12 level. J Hepatol 33, 791–798.[CrossRef]
    [Google Scholar]
  16. Green, S. J., Aniagolu, J. & Jraney, J. ( 1998;). Oxidative metabolism of murine macrophages. In Current Protocols in Immunology. Chapter 14, 5.1. Edited by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach & W. Strobers. New York: Wiley.
  17. Herman, A., Kappler, J. W., Marrack, P. & Pullen, A. M. ( 1991;). Superantigens: mechanism of T-cell stimulation and role in immune responses. Annu Rev Immunol 9, 745–772.[CrossRef]
    [Google Scholar]
  18. Islam, D., Wretlind, B., Ryd, M., Lindberg, A. A. & Christensson, B. ( 1995;). Immunoglobulin subclass distribution and dynamics of Shigella-specific antibody responses in serum and stool samples in shigellosis. Infect Immun 63, 2054–2061.
    [Google Scholar]
  19. Islam, D., Wretlind, B., Lindberg, A. A. & Christensson, B. ( 1996;). Changes in the peripheral blood T-cell receptor Vβ repertoire in vivo and in vitro during shigellosis. Infect Immun 64, 1391–1399.
    [Google Scholar]
  20. Jenkins, M. J., Chen, C., Jung, G., Mueller, D. L. & Schwartz, R. H. ( 1990;). Inhibition of antigen-specific proliferation of type 1 murine T cell clones after stimulation with immobilized anti-CD3 monoclonal antibody. J Immunol 144, 16–22.
    [Google Scholar]
  21. Johnston, K. H. & Gotschlich, E. C. ( 1974;). Isolation and characterization of the outer membrane of Neisseria gonorrhoeae. J Bacteriol 119, 250–257.
    [Google Scholar]
  22. Kaur, T., Singh, S., Dhawan, V. & Ganguly, N. K. ( 1998;). Shigella dysenteriae type 1 toxin induced lipid peroxidation in enterocytes isolated from rabbit ileum. Mol Cell Biochem 178, 169–179.[CrossRef]
    [Google Scholar]
  23. Kruisbeek, A. M. ( 1991;). In Current Protocols in Immunology, chapter 3, 12.1. Edited by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach & W. Strober. New York: Wiley-Interscience.
  24. Mallet, C. P., Hale, T. L., Kaminski, R. W., Larsen, T., Orr, N., Cohen, D. & Lowell, G. H. ( 1995;). Intransal or intragastric immunization with proteosomes-Shigella lipopolysaccaride vaccines protects against lethal pneumonia in a murine model of Shigella infection. Infect Immun 63, 2382–2386.
    [Google Scholar]
  25. Maluccio, M., Rao, J., Sharma, V. K., Lagman, M. & Suthanthiran, M. ( 2001). A novel immunoregulatory role for anti-CD3 mAbs: dendritic cells, pulsed with tumor cells and armed with anti-CD3 mAbs, are highly effective in constraining tumor progression. p. 815, abstract no. 512, American Society of Transplantation 18th Annual Scientific Meeting. Chicago, IL: AST.
  26. Markwell, M. A., Haas, S. M., Bieber, L. L. & Tolbert, M. E. ( 1978;). A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 87, 206–210.[CrossRef]
    [Google Scholar]
  27. Matsuyama, T., Yamada, A., Deusch, K., Sleasman, J., Daley, J. F., Torimoto, Y. & Abe, T. ( 1991;). Cytochalasins enhance the proliferation of CD4 cells through the CD3-Ti antigen receptor complex or the CD2 molecule through an effect on early events of activation. J Immunol 146, 3736–3741.
    [Google Scholar]
  28. Nguyen, D. D., Beck, L. & Spiegelberg, H. L. ( 1995;). Anti-CD3-induced anergy in cloned human Th0, Th1, and Th2 cells. Cell Immunol 165, 153–157.[CrossRef]
    [Google Scholar]
  29. Payne, S. M., Sharrow, S. O., Shearer, G. M. & Biddison, W. E. ( 1981;). Preparative separation of human T-cells reactive with the OKT4 monoclonal antibody. Int J Immunopharmacol 3, 227–232.[CrossRef]
    [Google Scholar]
  30. Raqib, R., Reinholt, F. P., Bardhan, P. K., Karnell, A. & Lindberg, A. A. ( 1994;). Immunopathological patterns in the rectal mucosa of patients with shigellosis: expression of HLA-DR antigens and T-lymphocyte subsets. APMIS 102, 371–380.[CrossRef]
    [Google Scholar]
  31. Raqib, R., Wretlind, B., Andersson, J. & Lindberg, A. A. ( 1995;). Cytokine secretion in acute shigellosis is correlated to disease activity and detected more to stool than to plasma. J Infect Dis 171, 376–384.[CrossRef]
    [Google Scholar]
  32. Raqib, R., Ekberg, C., Sharkar, P., Bardhan, P. K., Zychlinsky, A., Sansonetti, P. J. & Andersson, J. ( 2002;). Apoptosis in acute shigellosis is associated with increase production of Fas/Fas ligand perforin, caspase-1 and caspase-3 but reduced production of Bcl-2 and interleukin-2. Infect Immun 70, 3199–3207.[CrossRef]
    [Google Scholar]
  33. Roilides, E., Mertins, S., Eddy, J., Walsh, T. J., Pizzo, P. A. & Rubin, M. ( 1990;). Impairment of neutrophil chemotactic and bactericidal function in children infected with human immunodeficiency virus type 1 and partial reversal after in vitro exposure to granulocyte-macrophage colony stimulating factor. J Pediatr 117, 531–540.[CrossRef]
    [Google Scholar]
  34. Rose-John, S. & Heinrich, P. C. ( 1994;). Soluble receptors for cytokines and growth factors: generation and biological function. Biochem J 300, 281–290.
    [Google Scholar]
  35. Sereny, B. ( 1957;). Experimental kerato-conjunctivities shigellosis. Acta Microbiol Acad Sci Hung 4, 367–376.
    [Google Scholar]
  36. Sinha, A. K. & Chakraborti, S. ( 1992;). Immunoblot analysis of antibody responses to Shigella dysenteriae type 1 infection. Indian J Med Res 95, 112–114.
    [Google Scholar]
  37. Sinha, A. K., Chakraborti, S. & Chakraborti, M. K. ( 1994;). Delayed hypersensitivity in relation to host cellular immune response in Shigella infected mice. Immunol Infect Dis 4, 149–152.
    [Google Scholar]
  38. Tamura, T., Yanagida, T. & Nariuchi, H. ( 1993;). Difference in signal transduction pathway for IL-2 and IL-4 production in T helper 1 and T helper 2 cell clones in response to anti-CD3. J Immunol 151, 6051–6061.
    [Google Scholar]
  39. Taniguchi, T. & Minami, Y. ( 1993;). The IL-2/IL-2R receptor system: a current overview. Cell 73, 5–8.[CrossRef]
    [Google Scholar]
  40. Voller, A., Bartlett, A. & Bidwell, D. E. ( 1978;). Enzyme immunoassays with special reference to ELISA techniques. J Clin Pathol 31, 507–520.[CrossRef]
    [Google Scholar]
  41. Watanabe, T., Inoue, T., Ochi, H., Terashima, M., Asano, Y. & Nakatani, T. ( 1999;). Lipid A directly inhibits IL-4 production by murine Th2 cells but does not inhibit IFN-γ production by Th1 cells. Eur J Immunol 29, 413–418.[CrossRef]
    [Google Scholar]
  42. Way, S. S., Borczuk, C. A., Dominitz, R. & Goldberg, M. B. ( 1998;). An essential role of gamma interferon in innate resistance to Shigella flexneri infection. Infect Immun 66, 1342–1348.
    [Google Scholar]
  43. WHO ( 1993). Manual for laboratory investigations of acute enteric infections. Publication CDD 83.3. Geneva: World Health Organization.
  44. WHO ( 1999). Vaccinology and Biotechnology applied to Infectious Diseases. Module 5 (Practicals) 4–7 October. Advanced Course in Immunology. Lausanne, Switzerland: WHO-IRTC.
  45. Yanagida, T., Kato, T., Igarashi, O., Inoue, T. & Nariuchi, H. ( 1994;). Second signal activity of IL-12 on the proliferation and IL-2R expression of T helper cell-1 clone. J Immunol 152, 4919–4928.
    [Google Scholar]
  46. Yin, E. T., Galanos, C., Kinsky, S., Bradshaw, R. A., Wessler, S., Luderritz, O. & Sarmiento, M. E. ( 1972;). Picogram sensitivity assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from Gram-negative bacteria. Biochim Biophys Acta 261, 284–289.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05420-0
Loading
/content/journal/jmm/10.1099/jmm.0.05420-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error