RT Journal Article SR Electronic(1) A1 Nolte, Oliver A1 Müller, Matthias A1 Reitz, Stephan A1 Ledig, Sandra A1 Ehrhard, Ingrid A1 Sonntag, Hans-GüntherYR 2003 T1 Description of new mutations in the rpoB gene in rifampicin-resistant Neisseria meningitidis selected in vitro in a stepwise manner JF Journal of Medical Microbiology, VO 52 IS 12 SP 1077 OP 1081 DO https://doi.org/10.1099/jmm.0.05371-0 PB Microbiology Society, SN 1473-5644, AB Fourteen meningococcal strains were selected towards rifampicin resistance in a stepwise manner in vitro; final MICs were between 8 and >256 μg ml−1. Sequence analysis of a 295 bp subgenic fragment of the RNA polymerase β-subunit (rpoB) gene from the original and the fully resistant strains revealed that, with one exception, the strain pairs differed by just one position in the deduced amino acid sequence. Transformation of a PCR-amplified subgenic rpoB fragment harbouring the mutated site into a susceptible strain demonstrated the resistance-conferring mechanism., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.05371-0