1887

Abstract

The Belgian Listeria Reference Centre receives between 30 and 50 human clinical strains of per year. In general, epidemiological data are absent or incomplete, preventing recognition of episodes of listeriosis. However, data on a clonal relationship between strains can indirectly give an idea of the occurrence of episodes. Human isolates of from 2001 were serotyped, their arsenic-cadmium resistance profiles were determined, and they were pulsotyped with the application of pulsed-field gel electrophoresis using I and I restriction endonucleases. On five occasions, two or more strains presented the same serovar, metal-resistance profile and pulsovar, suggesting a clonal relationship. This is the first report to identify accurately potential listeriosis episodes occurring in Belgium.

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2004-05-01
2019-11-22
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References

  1. Autio, T., Lundén, J., Frederiksson-Ahomaa, M., Björkroth, J., Sjöberg, A.-M. & Korkeala, H. ( 2002;). Similar Listeria monocytogenes pulsotypes detected in several foods originating from different sources. Int J Food Microbiol 77, 83–90.[CrossRef]
    [Google Scholar]
  2. Boerlin, P., Boerlin-Petzold, F., Bannerman, E., Bille, J. & Jemmi, T. ( 1997;). Typing Listeria monocytogenes isolates from fish products and human listeriosis cases. Appl Environ Microbiol 63, 1338–1343.
    [Google Scholar]
  3. Brosch, R., Buchrieser, C. & Rocourt, J. ( 1991;). Subtyping of Listeria monocytogenes serovar 4b by use of low-frequency-cleavage restriction endonuclease and pulsed-field gel electrophoresis. Res Microbiol 142, 667–675.[CrossRef]
    [Google Scholar]
  4. Brosch, R., Brett, M., Catimel, B., Luchansky, J. B., Ojeniyi, B. & Rocourt, J. ( 1996;). Genomic fingerprinting of 80 strains from the WHO multicentre international typing study of Listeria monocytogenes via pulsed-field gel electrophoresis (PFGE). Int J Food Microbiol 32, 343–355.[CrossRef]
    [Google Scholar]
  5. Buchrieser, C., Brosch, R. & Rocourt, J. ( 1991;). Use of pulsed field gel electrophoresis to compare large DNA-restriction fragments of Listeria monocytogenes strains belonging to serogroups 1/2 and 3. Int J Food Microbiol 14, 297–304.[CrossRef]
    [Google Scholar]
  6. Gianfranceschi, M., Pourshaban, M., Gattuso, A., Wedell-Neergaard, C. & Aureli, P. ( 2002;). Characterization of Listeria monocytogenes strains isolated from food and humans in Italy by pulsed-field gel electrophoresis. Food Microbiol 19, 47–55.
    [Google Scholar]
  7. Graves, L. M., Swaminathan, B., Reeves, M. W. & Wenger, J. ( 1991;). Ribosomal DNA fingerprinting of Listeria monocytogenes using a digoxigenin-labelled DNA probe. Eur J Epidemiol 7, 77–82.
    [Google Scholar]
  8. Graves, L. M. & Swaminathan, B. ( 2001;). PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis. Int J Food Microbiol 65, 55–62.[CrossRef]
    [Google Scholar]
  9. Graves, L. M., Swaminathan, B. & Hunter, S. B. ( 1999;). Subtyping Listeria monocytogenes. In Listeria, Listeriosis and Food Safety, 2nd edn, pp. 279–297. Edited by E. T. Ryser & E. H. Marth. New York: Marcel Dekker.
  10. Loesser, M. J. & Busse, M. ( 1990;). Bacteriophage typing of Listeria species. Appl Environ Microbiol 56, 1912–1918.
    [Google Scholar]
  11. Louie, M., Jayaratne, P., Luchsinger, I., Devenish, J., Yao, J., Schlech, W. & Simor, A. ( 1996;). Comparison of ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for molecular typing of Listeria monocytogenes. J Clin Microbiol 34, 15–19.
    [Google Scholar]
  12. Lozniewski, A., Humbert, A., Corsaro, D., Schwartzbrod, J., Weber, M. & Le Faou, A. ( 2001;). Comparison of sludge and clinical isolates of Listeria monocytogenes. Lett Appl Microbiol 32, 336–339.[CrossRef]
    [Google Scholar]
  13. Mazurier, S. I., Audurier, A., Marquet-Van der Mee, N., Notermans, S. & Wernars, K. ( 1992;). A comparative study of randomly amplified polymorphic DNA analysis and conventional phage typing for epidemiological studies of Listeria monocytogenes isolates. Res Microbiol 143, 507–512.[CrossRef]
    [Google Scholar]
  14. McLauchlin, J., Hampton, M. D., Shah, S., Threlfall, E. J., Wieneke, A. A. & Curtis, G. D. W. ( 1997;). Subtyping of Listeria monocytogenes on the basis of plasmid profiles and arsenic and cadmium susceptibility. J Appl Microbiol 83, 381–388.[CrossRef]
    [Google Scholar]
  15. Nakama, A., Terao, M., Kokubo, Y., Itoh, T., Maruyama, T., Kaneuchi, C. & McLauchlin, J. ( 1998;). A comparison of Listeria monocytogenes serovar 4b isolates of clinical and food origin in Japan by pulsed-field gel electrophoresis. Int J Food Microbiol 42, 201–206.[CrossRef]
    [Google Scholar]
  16. Ojeniyi, B., Christensen, J. & Bisgaard, M. ( 2000;). Comparative investigations of Listeria monocytogenes isolated from a turkey processing plant, turkey products, and from human cases of listeriosis in Denmark. Epidemiol Infect 125, 303–308.[CrossRef]
    [Google Scholar]
  17. Ridley, A. M. ( 1995;). Evaluation of a restriction fragment length polymorphism typing method for Listeria monocytogenes. Res Microbiol 146, 21–34.[CrossRef]
    [Google Scholar]
  18. Rocourt, J. & Bille, J. ( 1997;). Foodborne listeriosis. World Health Stat Q 50, 67–73.
    [Google Scholar]
  19. Seeliger, H. P. R. & Hohne, K. ( 1979;). Serotyping of L.monocytogenes and related species. Methods Microbiol 13, 31–49.
    [Google Scholar]
  20. Selander, R. K., Caugant, D. A., Ochman, H., Musser, J. M., Gilmour, M. N. & Whittam, T. S. ( 1986;). Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol 51, 873–884.
    [Google Scholar]
  21. Swaminathan, B., Barrett, T. J., Hunter, S. B. & Tauxe, R. V. ( 2001;). PulseNet: the molecular subtyping network for foodborne bacterial disease surveillance, United States.CDC PulseNet Task Force. Emerg Infect Dis 7, 382–389.[CrossRef]
    [Google Scholar]
  22. Vela, A. I., Fernandez-Garayzabal, J. F., Varquez, J. A. & 10 other authors ( 2001;). Molecular typing by pulsed-field gel electrophoresis of Spanish animal and human Listeria monocytogenes isolates. Appl Environ Microbiol 67, 5840–5843.[CrossRef]
    [Google Scholar]
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