1887

Abstract

We have reported previously that heat-shock protein 60 kDa (hsp60) of is an important antigen in the pathogenesis of gastric mucosa-associated lymphoid tissue (MALT) lymphoma. In order to investigate associations with host immune reactions and hsp60 antigen, CD40 ligand (CD40L) expression and cytokine production were analysed following stimulation with hsp60. To provide a clear antigen-driven immune response, peripheral blood mononuclear cells (PBMC) from patients with low-grade MALT lymphoma and gastritis and those from healthy volunteers were stimulated with recombinant hsp60 and cell lysate in the presence of cytokines (IL4 and granulocyte-macrophage colony-stimulating factor). mRNA expression was also analysed by a cDNA microarray containing 1100 genes. Expression of CD40L on PBMCs of patients with MALT lymphoma was increased by cytokines or by combination with stimulation with hsp60 antigens. The production of IL4 in PBMC cultures was increased in patients with MALT lymphoma; however, production of IFN-γ was at low levels. DNA microarray analysis indicated increased levels of HLA-DR and integrin mRNAs. In cases of low-grade MALT lymphoma, adaptive immune responses against hsp60 may be enhanced by host factors, such as antigen presentation and T-cell activation, resulting in B-cell proliferation, which can be demonstrated during chronic infection.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05348-0
2004-01-01
2019-11-17
Loading full text...

Full text loading...

/deliver/fulltext/jmm/53/1/JM530104.html?itemId=/content/journal/jmm/10.1099/jmm.0.05348-0&mimeType=html&fmt=ahah

References

  1. Ahlstedt, I., Lindholm, C., Lonroth, H., Hamlet, A., Svennerholm, A. M. & Quiding-Jarbrink, M. ( 1999;). Role of local cytokines in increased gastric expression of the secretory component in Helicobacter pylori infection. Infect Immun 67, 4921–4925.
    [Google Scholar]
  2. Ambrosini, G., Adida, C. & Altieri, D. C. ( 1997;). A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med 3, 917–921.[CrossRef]
    [Google Scholar]
  3. Ambrosini, G., Adida, C., Sirugo, G. & Altieri, D. C. ( 1998;). Induction of apoptosis and inhibition of cell proliferation by survivin gene targeting. J Biol Chem 273, 11177–11182.[CrossRef]
    [Google Scholar]
  4. Bayerdorffer, E., Neubauer, A., Rudolph, B., Thiede, C., Lehn, N., Eidt, S. & Stolte, M. ( 1995;). Regression of primary gastric lymphoma of mucosa-associated lymphoid tissue type after cure of Helicobacter pylori infection.MALT Lymphoma Study Group. Lancet 345, 1591–1594.[CrossRef]
    [Google Scholar]
  5. Cammarota, G., Montalto, M., Tursi, A., Vecchio, F. M., Fedeli, G. & Gasbarrini, G. ( 1995;). Helicobacter pylori reinfection and rapid relapse of low-grade B-cell gastric lymphoma. Lancet 345, 192. 192.
    [Google Scholar]
  6. Chen, B., Shi, Y., Smith, J. D., Choi, D., Geiger, J. D. & Mule, J. J. ( 1998;). The role of tumor necrosis factor alpha in modulating the quantity of peripheral blood-derived, cytokine-driven human dendritic cells and its role in enhancing the quality of dendritic cell function in presenting soluble antigens to CD4+ T cells in vitro. Blood 91, 4652–4661.
    [Google Scholar]
  7. Dogan, A., Du, M., Koulis, A., Briskin, M. J. & Isaacson, P. G. ( 1997;). Expression of lymphocyte homing receptors and vascular addressins in low-grade gastric B-cell lymphomas of mucosa-associated lymphoid tissue. Am J Pathol 151, 1361–1369.
    [Google Scholar]
  8. Duggan, D. J., Bittner, M., Chen, Y., Meltzer, P. & Trent, J. M. ( 1999;). Expression profiling using cDNA microarrays. Nat Genet 21 (Suppl.), 10–14.[CrossRef]
    [Google Scholar]
  9. Engstrand, L., Scheynius, A., Pahlson, C., Grimelius, L., Schwan, A. & Gustavsson, S. ( 1989;). Association of Campylobacter pylori with induced expression of class II transplantation antigens on gastric epithelial cells. Infect Immun 57, 827–832.
    [Google Scholar]
  10. Enno, A., O'Rourke, J. L., Howlett, C. R., Jack, A., Dixon, M. F. & Lee, A. ( 1995;). MALToma-like lesions in the murine gastric mucosa after long-term infection with Helicobacter felis.A mouse model of Helicobacter pylori-induced gastric lymphoma. Am J Pathol 147, 217–222.
    [Google Scholar]
  11. Greiner, A., Knorr, C., Qin, Y., Schultz, A., Marx, A., Kroczek, R. A. & Muller-Hermelink, H. K. ( 1998;). CD40 ligand and autoantigen are involved in the pathogenesis of low-grade B-cell lymphomas of mucosa-associated lymphoid tissue. Dev Immunol 6, 187–195.[CrossRef]
    [Google Scholar]
  12. Harris, N. L., Jaffe, E. S., Stein, H. & 7 other authors ( 1994;). A revised European-American classification of lymphoid neoplasms: a proposal from the International Lymphoma Study Group. Blood 84, 1361–1392.
    [Google Scholar]
  13. Hauer, A. C., Finn, T. M., MacDonald, T. T., Spencer, J. & Isaacson, P. G. ( 1997;). Analysis of TH1 and TH2 cytokine production in low grade B cell gastric MALT-type lymphomas stimulated in vitro with Helicobacter pylori. J Clin Pathol 50, 957–959.[CrossRef]
    [Google Scholar]
  14. Hida, N., Shimoyama, T., Jr, Neville, P., Dixon, M. F., Axon, A. T., Shimoyama, T., Sr & Crabtree, J. E. ( 1999;). Increased expression of IL-10 and IL-12 (p40) mRNA in Helicobacter pylori infected gastric mucosa: relation to bacterial cag status and peptic ulceration. J Clin Pathol 52, 658–664.[CrossRef]
    [Google Scholar]
  15. Horstmann, M., Erttmann, R. & Winkler, K. ( 1994;). Relapse of MALT lymphoma associated with Helicobacter pylori after antibiotic treatment. Lancet 343, 1098–1099.
    [Google Scholar]
  16. Hussell, T., Isaacson, P. G. & Spencer, J. ( 1993;). Proliferation and differentiation of tumour cells from B-cell lymphoma of mucosa-associated lymphoid tissue in vitro. J Pathol 169, 221–227.[CrossRef]
    [Google Scholar]
  17. Hussell, T., Isaacson, P. G., Crabtree, J. E. & Spencer, J. ( 1996;). Helicobacter pylori-specific tumour-infiltrating T cells provide contact dependent help for the growth of malignant B cells in low-grade gastric lymphoma of mucosa-associated lymphoid tissue. J Pathol 178, 122–127.[CrossRef]
    [Google Scholar]
  18. Isaacson, P. G. & Spencer, J. ( 1993;). Is gastric lymphoma an infectious disease? Hum Pathol 24, 569–570.[CrossRef]
    [Google Scholar]
  19. Jiang, W., Swiggard, W. J., Heufler, C., Peng, M., Mirza, A., Steinman, R. M. & Nussenzweig, M. C. ( 1995;). The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing. Nature 375, 151–155.[CrossRef]
    [Google Scholar]
  20. Katunuma, N., Kakegawa, H., Matsunaga, Y. & Saibara, T. ( 1994;). Immunological significances of invariant chain from the aspect of its structural homology with the cystatin family. FEBS Lett 349, 265–269.[CrossRef]
    [Google Scholar]
  21. Kawahara, Y., Yokota, K., Mizuno, M. & 7 other authors ( 1999;). Antibodies to human gastric epithelial cells and heat shock protein 60 in Helicobacter pylori positive mucosa associated lymphoid tissue lymphoma. Gut 45, 20–23.[CrossRef]
    [Google Scholar]
  22. Khan, J., Bittner, M. L., Saal, L. H., Teichmann, U., Azorsa, D. O., Gooden, G. C., Pavan, W. J., Trent, J. M. & Meltzer, P. S. ( 1999;). cDNA microarrays detect activation of a myogenic transcription program by the PAX3-FKHR fusion oncogene. Proc Natl Acad Sci U S A 96, 13264–13269.[CrossRef]
    [Google Scholar]
  23. Knorr, C., Amrehn, C., Seeberger, H., Rosenwald, A., Stilgenbauer, S., Ott, G., Muller Hermelink, H. K. & Greiner, A. ( 1999;). Expression of costimulatory molecules in low-grade mucosa-associated lymphoid tissue-type lymphomas in vivo. Am J Pathol 155, 2019–2027.[CrossRef]
    [Google Scholar]
  24. Kobayashi, K., Yokota, K., Yoshino, T., Kawahara, Y., Dey, A., Hirai, Y., Oguma, K. & Akagi, T. ( 1998;). Detection of Helicobacter pylori associated antigen and heat shock protein 60 on follicular dendritic cells in the germinal centres of low grade B cell lymphoma of gastric mucosa associated lymphoid tissue (MALT). J Clin Pathol 51, 396–398.[CrossRef]
    [Google Scholar]
  25. Koulis, A., Diss, T., Isaacson, P. G. & Dogan, A. ( 1997;). Characterization of tumor-infiltrating T lymphocytes in B-cell lymphomas of mucosa-associated lymphoid tissue. Am J Pathol 151, 1353–1360.
    [Google Scholar]
  26. Lederman, S., Yellin, M. J., Krichevsky, A., Belko, J., Lee, J. J. & Chess, L. ( 1992;). Identification of a novel surface protein on activated CD4+ T cells that induces contact-dependent B cell differentiation (help). J Exp Med 175, 1091–1101.[CrossRef]
    [Google Scholar]
  27. Lindholm, C., Quiding-Jarbrink, M., Lonroth, H., Hamlet, A. & Svennerholm, A. M. ( 1998;). Local cytokine response in Helicobacter pylori-infected subjects. Infect Immun 66, 5964–5971.
    [Google Scholar]
  28. Meyer, F., Wilson, K. T. & James, S. P. ( 2000;). Modulation of innate cytokine responses by products of Helicobacter pylori. Infect Immun 68, 6265–6272.[CrossRef]
    [Google Scholar]
  29. Oberhuber, G., Bodingbauer, M., Mosberger, I., Stolte, M. & Vogelsang, H. ( 1998;). High proportion of granzyme B-positive (activated) intraepithelial and lamina propria lymphocytes in lymphocytic gastritis. Am J Surg Pathol 22, 450–458.[CrossRef]
    [Google Scholar]
  30. Ohashi, K., Burkart, V., Flohe, S. & Kolb, H. ( 2000;). Cutting edge: heat shock protein 60 is a putative endogenous ligand of the toll-like receptor-4 complex. J Immunol 164, 558–561.[CrossRef]
    [Google Scholar]
  31. Parsonnet, J., Hansen, S., Rodriguez, L., Gelb, A. B., Warnke, R. A., Jellum, E., Orentreich, N., Vogelman, J. H. & Friedman, G. D. ( 1994;). Helicobacter pylori infection and gastric lymphoma. N Engl J Med 330, 1267–1271.[CrossRef]
    [Google Scholar]
  32. Romagnani, S. ( 1996;). Understanding the role of Th1/Th2 cells in infection. Trends Microbiol 4, 470–473.[CrossRef]
    [Google Scholar]
  33. Sandberg, J. K., Fast, N. M. & Nixon, D. F. ( 2001;). Functional heterogeneity of cytokines and cytolytic effector molecules in human CD8+ T lymphocytes. J Immunol 167, 181–187.[CrossRef]
    [Google Scholar]
  34. Smythies, L. E., Waites, K. B., Lindsey, J. R., Harris, P. R., Ghiara, P. & Smith, P. D. ( 2000;). Helicobacter pylori-induced mucosal inflammation is Th1 mediated and exacerbated in IL-4, but not IFN-γ, gene-deficient mice. J Immunol 165, 1022–1029.[CrossRef]
    [Google Scholar]
  35. Tsai, V., Southwood, S., Sidney, J., Sakaguchi, K., Kawakami, Y., Appella, E., Sette, A. & Celis, E. ( 1997;). Identification of subdominant CTL epitopes of the GP100 melanoma-associated tumor antigen by primary in vitro immunization with peptide-pulsed dendritic cells. J Immunol 158, 1796–1802.
    [Google Scholar]
  36. Vabulas, R. M., Ahmad-Nejad, P., da Costa, C., Miethke, T., Kirschning, C. J., Hacker, H. & Wagner, H. ( 2001;). Endocytosed HSP60s use toll-like receptor 2 (TLR2) and TLR4 to activate the toll/interleukin-1 receptor signaling pathway in innate immune cells. J Biol Chem 276, 31332–31339.[CrossRef]
    [Google Scholar]
  37. van den Biggelaar, A. H., Grogan, J. L., Filie, Y., Jordens, R., Kremsner, P. G., Koning, F. & Yazdanbakhsh, M. ( 2000;). Chronic schistosomiasis: dendritic cells generated from patients can overcome antigen-specific T cell hyporesponsiveness. J Infect Dis 182, 260–265.[CrossRef]
    [Google Scholar]
  38. van Gelder, R. N., von Zastrow, M. E., Yool, A., Dement, W. C., Barchas, J. D. & Eberwine, J. H. ( 1990;). Amplified RNA synthesized from limited quantities of heterogeneous cDNA. Proc Natl Acad Sci U S A 87, 1663–1667.[CrossRef]
    [Google Scholar]
  39. Wang, K., Gan, L., Jeffery, E. & 8 other authors ( 1999;). Monitoring gene expression profile changes in ovarian carcinomas using cDNA microarray. Gene 229, 101–108.[CrossRef]
    [Google Scholar]
  40. Wang, J., Blanchard, T. G. & Ernst, P. B. ( 2001;). Host inflammatory response to infection. In Helicobacter pylori Physiology and Genetics, pp. 471–480. Edited by H. L. T. Mobley, G. L. Mendz & S. L. Hazell. Washington, DC: American Society for Microbiology.
  41. Wotherspoon, A. C., Ortiz-Hidalgo, C., Falzon, M. R. & Isaacson, P. G. ( 1991;). Helicobacter pylori-associated gastritis and primary B-cell gastric lymphoma. Lancet 338, 1175–1176.[CrossRef]
    [Google Scholar]
  42. Wotherspoon, A. C., Doglioni, C., Diss, T. C., Pan, L., Moschini, A., de Boni, M. & Isaacson, P. G. ( 1993;). Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori. Lancet 342, 575–577.[CrossRef]
    [Google Scholar]
  43. Yasumoto, K., Okamoto, S., Mukaida, N., Murakami, S., Mai, M. & Matsushima, K. ( 1992;). Tumor necrosis factor α and interferon γ synergistically induce interleukin 8 production in a human gastric cancer cell line through acting concurrently on AP-1 and NF-κB-like binding sites of the interleukin 8 gene. J Biol Chem 267, 22506–22511.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05348-0
Loading
/content/journal/jmm/10.1099/jmm.0.05348-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error