1887

Abstract

Thirty-seven multidrug-resistant and 13 pan-susceptible isolates of were analysed for the diversity of genotypes associated with known drug-resistance mechanisms. The isolates were obtained from patients attending a university tuberculosis clinic in Monterrey, Mexico. A total of 25 IS-RFLP patterns were obtained from the multidrug-resistant tuberculosis (MDR-TB) isolates. Approximately 65 % of the MDR-TB isolates were attributed to secondary resistance. Different drug-susceptibility patterns were seen with the clustered isolates. The percentage of isolates resistant to isoniazid (INH), rifampicin (RIF), ethambutol (EMB) and streptomycin (STR) was 100, 97.3, 48.7 and 67.6, respectively. The most common resistance-associated polymorphisms for the four drugs were as follows: INH, Ser315Thr (67.6 %) in ; RIF, Ser450Leu (41.7 %) in ; EMB, Met306Ile/Val/Leu (66.7 %) in ; and STR, Lys43Arg (24 %) in . Drug-resistance-associated mutations were similar to changes occurring in isolates from other areas of the world, but unique, previously unreported, mutations in ( = 5), ( = 1) and ( = 3) were also identified.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05343-0
2004-02-01
2019-11-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/53/2/JM530205.html?itemId=/content/journal/jmm/10.1099/jmm.0.05343-0&mimeType=html&fmt=ahah

References

  1. Anonymous ( 1998a;). Population-based survey for drug resistance of tuberculosis – Mexico, 1997. MMWR Morb Mortal Wkly Rep 47, 371–375.
    [Google Scholar]
  2. Anonymous ( 1998b;). Guidelines for surveillance of drug resistance in tuberculosis.WHO Geneva/IUATLD Paris. International Union Against Tuberculosis and Lung Disease. Int J Tuberc Lung Dis 2, 72–89.
    [Google Scholar]
  3. Chaves, F., Yang, Z. H., El Hajj, H., Alonso, M., Burman, W. J., Eisenach, K. D., Dronda, F., Bates, J. H. & Cave, M. D. ( 1996;). Usefulness of the secondary probe pTBN12 in DNA fingerprinting of Mycobacterium tuberculosis. J Clin Microbiol 34, 1118–1123.
    [Google Scholar]
  4. Cole, S. T., Brosch, R., Parkhill, J. & 39 other authors. ( 1998;). Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393, 537–544.[CrossRef]
    [Google Scholar]
  5. Escalante, P., Ramaswamy, S., Sanabria, H., Soini, H., Pan, X., Valiente-Castillo, O. & Musser, J. M. ( 1998;). Genotypic characterization of drug-resistant Mycobacterium tuberculosis isolates from Peru. Tuber Lung Dis 79, 111–118.[CrossRef]
    [Google Scholar]
  6. Heym, B., Alzari, P. M., Honoré, N. & Cole, S. T. ( 1995;). Missense mutations in the catalase-peroxidase gene, katG, are associated with isoniazid resistance in Mycobacterium tuberculosis. Mol Microbiol 15, 235–245.[CrossRef]
    [Google Scholar]
  7. Jin, D. J. & Gross, C. A. ( 1988;). Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance. J Mol Biol 202, 45–58.[CrossRef]
    [Google Scholar]
  8. Kapur, V., Li, L. L., Iordanescu, S., Hamrick, M. R., Wanger, A., Kreiswirth, B. N. & Musser, J. M. ( 1994;). Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase β subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas. J Clin Microbiol 32, 1095–1098.
    [Google Scholar]
  9. Marttila, H. J., Soini, H., Eerola, E., Vyshnevskaya, E., Vyshnevskiy, B. I., Otten, T. F., Vasilyef, A. V. & Viljanen, M. K. ( 1998;). A Ser315Thr substitution in KatG is predominant in genetically heterogeneous multidrug-resistant Mycobacterium tuberculosis isolates originating from the St.Petersburg area in Russia. Antimicrob Agents Chemother 42, 2443–2445.
    [Google Scholar]
  10. Meier, A., Kirschner, P., Bange, F.-C., Vogel, U. & Böttger, E. C. ( 1994;). Genetic alterations in streptomycin-resistant Mycobacterium tuberculosis: mapping of mutations conferring resistance. Antimicrob Agents Chemother 38, 228–233.[CrossRef]
    [Google Scholar]
  11. Nair, J., Rouse, D. A., Bai, G.-H. & Morris, S. L. ( 1993;). The rpsL gene and streptomycin resistance in single and multiple drug-resistant strains of Mycobacterium tuberculosis. Mol Microbiol 10, 521–527.[CrossRef]
    [Google Scholar]
  12. National Committee for Clinical Laboratory Standards ( 1995;). Antimycobacterial Susceptibility Testing for Mycobacterium tuberculosis. Proposed Standard M24-T. Villanova, PA: National Committee for Clinical Laboratory Standards.
  13. Ramaswamy, S. & Musser, J. M. ( 1998;). Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuber Lung Dis 79, 3–29.[CrossRef]
    [Google Scholar]
  14. Ramaswamy, S. V., Amin, A. G., Göksel, S., Stager, C. E., Dou, S.-J., El Sahly, H., Moghazeh, S. L., Kreiswirth, B. N. & Musser, J. M. ( 2000;). Molecular genetic analysis of nucleotide polymorphisms associated with ethambutol resistance in human isolates of Mycobacterium tuberculosis. Antimicrob Agents Chemother 44, 326–336.[CrossRef]
    [Google Scholar]
  15. Raviglione, M. C., Snider, D. E., Jr & Kochi, A. ( 1995;). Global epidemiology of tuberculosis.Morbidity and mortality of a worldwide epidemic. JAMA (J Am Med Assoc) 273, 220–226.[CrossRef]
    [Google Scholar]
  16. Rinder, H., Dobner, P., Feldmann, K., Rifai, M., Bretzel, G., Rusch-Gerdes, S. & Loscher, T. ( 1997;). Disequilibria in the distribution of rpoB alleles in rifampicin-resistant M.tuberculosis isolates from Germany and Sierra Leone. Microb Drug Resist 3, 195–197.[CrossRef]
    [Google Scholar]
  17. Sreevatsan, S., Pan, X., Stockbauer, K. E., Williams, D. L., Kreiswirth, B. N. & Musser, J. M. ( 1996;). Characterization of rpsL and rrs mutations in streptomycin-resistant Mycobacterium tuberculosis isolates from diverse geographic localities. Antimicrob Agents Chemother 40, 1024–1026.
    [Google Scholar]
  18. Sreevatsan, S., Pan, X., Stockbauer, K. E., Connell, N. D., Kreiswirth, B. N., Whittam, T. S. & Musser, J. M. ( 1997a;). Restricted structural gene polymorphism in the Mycobacterium tuberculosis complex indicates evolutionarily recent global dissemination. Proc Natl Acad Sci U S A 94, 9869–9874.[CrossRef]
    [Google Scholar]
  19. Sreevatsan, S., Stockbauer, K. E., Pan, X., Kreiswirth, B. N., Moghazeh, S. L., Jacobs, W. R., Jr, Telenti, A. & Musser, J. M. ( 1997b;). Ethambutol resistance in Mycobacterium tuberculosis: critical role of embB mutations. Antimicrob Agents Chemother 41, 1677–1681.
    [Google Scholar]
  20. Telenti, A., Imboden, P., Marchesi, F., Lowrie, D., Cole, S., Colston, M. J., Matter, L., Schopfer, K. & Bodmer, T. ( 1993;). Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis. Lancet 341, 647–650.[CrossRef]
    [Google Scholar]
  21. Telenti, A., Honore, N., Bernasconi, C., March, J., Ortega, A., Heym, B., Takiff, H. E. & Cole, S. T. ( 1997a;). Genotypic assessment of isoniazid and rifampin resistance in Mycobacterium tuberculosis: a blind study at reference laboratory level. J Clin Microbiol 35, 719–723.
    [Google Scholar]
  22. Telenti, A., Philipp, W. J., Sreevatsan, S., Bernasconi, C., Stockbauer, K. E., Wieles, B., Musser, J. M. & Jacobs, W. R., Jr ( 1997b;). The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol. Nat Med 3, 567–570.[CrossRef]
    [Google Scholar]
  23. van Embden, J. D., Cave, M. D., Crawford, J. T. & 8 other authors ( 1993;). Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 31, 406–409.
    [Google Scholar]
  24. Viader-Salvadó, J. M., Luna-Aguirre, C. M., Reyes-Ruiz, J. M., Valdez-Leal, R., Bosque-Moncayo, M. D. L. A. D., Tijerina-Menchaca, R. & Guerrero-Olazarán, M. ( 2003;). Frequency of mutations in rpoB and codons 315 and 463 of katG in rifampin- and/or isoniazid-resistant Mycobacterium tuberculosis isolates from northeast Mexico. Microb Drug Resist 9, 33–38.[CrossRef]
    [Google Scholar]
  25. Victor, T. C., van Rie, A., Jordaan, A. M., Richardson, M., van der Spuy, G. D., Beyers, N., van Helden, P. D. & Warren, R. ( 2001;). Sequence polymorphism in the rrs gene of Mycobacterium tuberculosis is deeply rooted within an evolutionary clade and is not associated with streptomycin resistance. J Clin Microbiol 39, 4184–4186.[CrossRef]
    [Google Scholar]
  26. World Health Organization ( 1997;). Anti-tuberculosis Drug Resistance in the World. The WHO/IUATLD Global Project on Anti-tuberculosis Drug Resistance Surveillance, 1994–1997. WHO/TB/97.229. Geneva: WHO Global Tuberculosis Programme.
  27. Yang, Z. H., Rendon, A., Flores, A. & 7 other authors ( 2001;). A clinic-based molecular epidemiologic study of tuberculosis in Monterrey, Mexico. Int J Tuberc Lung Dis 5, 313–320.
    [Google Scholar]
  28. Zhang, Y., Heym, B., Allen, B., Young, D. & Cole, S. ( 1992;). The catalase-peroxidase gene and isoniazid resistance of Mycobacterium tuberculosis. Nature 358, 591–593.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05343-0
Loading
/content/journal/jmm/10.1099/jmm.0.05343-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error