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Volume 62, Issue 3

The dermatophyte species : intraspecies variability and mating behaviour Free

Abstract

is a zoophilic dermatophyte belonging to the species complex. Here, a population of wild strains from the same geographical area (Switzerland) was studied by comparing their morphology, assessing their molecular variability using internal transcribed spacer (ITS) and 28S rRNA gene sequencing, and evaluating their interfertility. Sequencing of the ITS region and of part of the 28S rRNA gene revealed the existence of two infraspecific groups with markedly different colony phenotypes: white (group I) and yellow (group II), respectively. For all strains, the results of mating type identification by PCR, using (high-mobility group) and α-box genes in the mating type locus as targets, were in total accordance with the results of mating type identification by strain confrontation experiments. White-phenotype strains were of mating type + (mt+) or mating type − (mt−), whilst yellow-phenotype strains were all mt−. White and yellow strains were found to produce fertile cleistothecia after mating with reference tester strains, which belonged to a third group intermediate between groups I and II. However, no interfertility was observed between yellow strains and white strains of mt+. A significant result was that white strains of mt− were able to mate and produce fertile cleistothecia with the white strain CBS 112371 (mt+), the genome of which has recently been sequenced and annotated. This finding should offer new tools for investigating the biology and genetics of dermatophytes using wild-type strains.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-03-01
2024-04-19
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The dermatophyte species Arthroderma benhamiae: intraspecies variability and mating behaviour
J. Med. Microbiol. 62, 377 (2013); https://doi.org/10.1099/jmm.0.053223-0
/content/journal/jmm/10.1099/jmm.0.053223-0
/content/journal/jmm/10.1099/jmm.0.053223-0
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