1887

Abstract

Fluorescence polarization immunoassay (FPA) uses molecular rotational properties to measure antibody binding to antigen directly. The potential use of this method was assessed in comparison to a competitive enzyme immunoassay (CELISA) and conventional serological tests for the diagnosis of brucellosis on a total of 587 human sera. Based on 340 sera from asymptomatic blood donors with no evidence of brucellosis, the specificity of the FPA was 97.9 % using a cut-off value of 72 mP. Sera from -infected patients (11 , 32 , 32 and one sp.) yielded a sensitivity estimate of 96.1 %. In tests on 84 sera from suspected brucellosis patients, the FPA detected 80 cases. Of 87 sera from patients with probable infection, 15 were detected by both CELISA and FPA, three by CELISA only and four by FPA only. The discrepancies in both groups involved sera with low, declining titres. The FPA uses a sample of 40 μl serum, takes about 5 min to complete and has been demonstrated to be accurate for the detection of antibodies to . , and and for identifying patients suffering relapses. Because of the ease of the procedure, it could be readily adopted for use in clinical laboratories and blood banks.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05217-0
2003-10-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jmm/52/10/jm521006.html?itemId=/content/journal/jmm/10.1099/jmm.0.05217-0&mimeType=html&fmt=ahah

References

  1. Alton, G. G., Jones, L. M., Angus, R. D. & Verger, J. M. ( 1988;). Serological methods. In Techniques for the Brucellosis Laboratory, pp. 63–134. Paris: Institut National de la Recherche Agronomique.
  2. Angus, R. D. & Barton, C. E. ( 1984;). The production and evaluation of a buffered plate antigen for use in a presumptive test for brucellosis. Dev Biol Stand 56, 349–356.
    [Google Scholar]
  3. Araj, G. F. ( 1999;). Human brucellosis: a classical infectious disease with persistent diagnostic challenges. Clin Lab Sci 12, 207–212.
    [Google Scholar]
  4. Ariza, J., Corredoira, J., Pallares, R., Viladrich, P. F., Rufi, G., Pujol, M. & Gudiol, F. ( 1995;). Characteristics of and risk factors for relapse of brucellosis in humans. Clin Infect Dis 20, 1241–1249.[CrossRef]
    [Google Scholar]
  5. Centro Panamericano de Zoonosis ( 1981;). Brucelosis: prueba de fijación de complemento. Nota Técnica no. 24. Buenos Aires: CEPANZO (in Spanish).
  6. Corbel, M. J. & Brinley-Morgan, W. J. ( 1984;). Genus Brucella Meyer and Shaw 1920, 173AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 377–388. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  7. Corbel, M. J., Thomas, E. L. & Garcia-Carrillo, C. ( 1984;). Taxonomic studies on some atypical strains of Brucella suis. Br Vet J 140, 34–43.[CrossRef]
    [Google Scholar]
  8. Garcia-Carrillo, C. & Lucero, N. E. ( 1993;). Diagnóstico bacteriológico. In Brucelosis Bovina, pp. 97–98. Edited by A. I. De Diego. Buenos Aires: Hemisferio Sur (in Spanish).
  9. Lucero, N. E. & Bolpe, J. E. ( 1998;). Buffered plate antigen test as a screening test for diagnosis of human brucellosis. J Clin Microbiol 36, 1425–1427.
    [Google Scholar]
  10. Lucero, N. E., Foglia, L., Ayala, S. M., Gall, D. & Nielsen, K. ( 1999;). Competitive enzyme immunoassay for diagnosis of human brucellosis. J Clin Microbiol 37, 3245–3248.
    [Google Scholar]
  11. Metz, C. E. ( 1978;). Basic principles of ROC analysis. Semin Nucl Med 8, 283–298.[CrossRef]
    [Google Scholar]
  12. Nielsen, K. H., Kelly, L., Gall, D., Nicoletti, P. & Kelly, W. ( 1995;). Improved competitive enzyme immunoassay for the diagnosis of bovine brucellosis. Vet Immunol Immunopathol 46, 285–291.[CrossRef]
    [Google Scholar]
  13. Nielsen, K. H., Gall, D., Kelly, W., Vigliocco, A., Henning, D. & Garcia, M. ( 1996;). Immunoassay Development: Application to Enzyme Immunoassay for the Diagnosis of Brucellosis. Nepean, Ontario: Agriculture and Agri-Food Canada.
  14. Nielsen, K., Lin, M., Gall, D. & Jolley, M. ( 2000;). Fluorescence polarization immunoassay: detection of antibody to Brucella abortus. Methods 22, 71–76.[CrossRef]
    [Google Scholar]
  15. Osoba, A. O., Balkhy, H., Memish, Z., Khan, M. Y., Al-Thagafi, A., Al Shareef, B., Al Mowallad, A. & Oni, G. A. ( 2001;). Diagnostic value of Brucella ELISA IgG and IgM in bacteremic and non-bacteremic patients with brucellosis. J Chemother 13 (Suppl. 1), 54–59.[CrossRef]
    [Google Scholar]
  16. Schoonjans, F., Zalata, A., Depuydt, C. E. & Comhaire, F. H. ( 1995;). MedCalc: a new computer program for medical statistics. Comput Methods Programs Biomed 48, 257–262.[CrossRef]
    [Google Scholar]
  17. Solera, J., Martinez-Alfaro, E., Espinoza, A., Castillejos, M., L., Geijo, P. & Rodriguez-Zapata, M. ( 1998;). Multivariate model for predicting relapse in human brucellosis. J Infect 36, 85–92.[CrossRef]
    [Google Scholar]
  18. Yagupsky, P. ( 1999;). Detection of brucellae in blood cultures. J Clin Microbiol 37, 3437–3442.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05217-0
Loading
/content/journal/jmm/10.1099/jmm.0.05217-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error