1887

Abstract

Three nationwide multicentre studies ( = 5071) showed an increase in antibiotic resistance in pneumococci in Germany. Serotype 23F was the predominant serotype ( = 45, 22.4 %), followed by 6B ( = 30, 14.9 %) and 9V ( = 19, 9.5 %). Multilocus sequence typing was used to characterize 45 serotype 23F strains with reduced penicillin susceptibility. The Spanish-1 clone [profile 4-4-2-4-4-1-1, sequence type (ST) 81] contributes significantly to the emergence of penicillin resistance in Germany ( = 21, 46.7 % of all penicillin non-susceptible serotype 23F isolates). Isolates of ST 277 (profile 7-13-8-6-6-12-8), which has been found previously in the Netherlands, are also observed, particularly in western Germany ( = 8, 17.8 %). A high proportion of strains ( = 11, 24.4 %) have sequence types that have not been reported to date from other countries (STs 353–362). The major penicillin-resistant clones are present in Germany, a country with relatively low levels of β-lactam resistance.

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2003-11-01
2019-11-12
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References

  1. Andrews, J., Ashby, J., Jevons, G., Lines, N. & Wise, R. ( 1999;). Antimicrobial resistance in gram-positive pathogens isolated in the UK between October 1996 and January 1997. J Antimicrob Chemother 43, 689–698.[CrossRef]
    [Google Scholar]
  2. Cars, O., Molstad, S. & Melander, A. ( 2001;). Variation in antibiotic use in the European Union. Lancet 357, 1851–1853.[CrossRef]
    [Google Scholar]
  3. Coffey, T. J., Berrón, S., Daniels, M., Garcia-Leoni, M. E., Cercenado, E., Bouza, E., Fenoll, A. & Spratt, B. G. ( 1996;). Multiply antibiotic-resistant Streptococcus pneumoniae recovered from Spanish hospitals (1988–1994): novel major clones of serotypes 14, 19F and 15F. Microbiology 142, 2747–2757.[CrossRef]
    [Google Scholar]
  4. Enright, M. C. & Spratt, B. G. ( 1998;). A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology 144, 3049–3060.[CrossRef]
    [Google Scholar]
  5. Granizo, J. J., Aguilar, L., Casal, J., Dal-Ré, R. & Baquero, F. ( 2000;). Streptococcus pyogenes resistance to erythromycin in relation to macrolide consumption in Spain (1986–1997). J Antimicrob Chemother 46, 959–964.[CrossRef]
    [Google Scholar]
  6. Henriques, B., Kalin, M., Örtqvist, Å. & 8 other authors ( 2000;). Molecular epidemiology of Streptococcus pneumoniae causing invasive disease in 5 countries. J Infect Dis 182, 833–839.[CrossRef]
    [Google Scholar]
  7. Hermans, P. W. M., Sluijter, M., Elzenaar, K. & 8 other authors ( 1997;). Penicillin-resistant Streptococcus pneumoniae in the Netherlands: results of a 1-year molecular epidemiologic survey. J Infect Dis 175, 1413–1422.[CrossRef]
    [Google Scholar]
  8. Johnson, A. P. ( 1998;). Antibiotic resistance among clinically important gram-positive bacteria in the UK. J Hosp Infect 40, 17–26.[CrossRef]
    [Google Scholar]
  9. Klugman, K., Goldstein, F., Kohno, S. & Baquero, F. ( 1997;). The role of fourth-generation cephalosporins in the treatment of infections caused by penicillin-resistant streptococci. Clin Microbiol Infect 3 (Suppl. 1), S48–S60.[CrossRef]
    [Google Scholar]
  10. Maiden, M. C. J., Bygraves, J. A., Feil, E. & 10 other authors ( 1998;). Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci U S A 95, 3140–3145.[CrossRef]
    [Google Scholar]
  11. McGee, L., McDougal, L., Zhou, J. & 8 other authors ( 2001;). Nomenclature of major antimicrobial-resistant clones of Streptococcus pneumoniae defined by the pneumococcal molecular epidemiology network. J Clin Microbiol 39, 2565–2571.[CrossRef]
    [Google Scholar]
  12. NCCLS ( 2000;). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically (M7-A4); Approved Standard (M100-S8), 4th edn. Wayne, PA: NCCLS.
  13. Neufeld, F. ( 1902;). Über die Agglutination der Pneumokokken und über die Theorie der Agglutination. Z Hyg Infektionskr 34, 54–72 (in German).
    [Google Scholar]
  14. Nielsen, S. V. & Henrichsen, J. ( 1992;). Capsular types of Streptococcus pneumoniae isolated from blood and CSF during 1982–1987. Clin Infect Dis 15, 794–798.[CrossRef]
    [Google Scholar]
  15. Pradier, C., Dunais, B., Carsenti-Etesse, H. & Dellamonica, P. ( 1997;). Pneumococcal resistance patterns in Europe. Eur J Clin Microbiol Infect Dis 16, 644–647.[CrossRef]
    [Google Scholar]
  16. Provvedi, R., Manganelli, R. & Pozzi, G. ( 1996;). Characterization of conjugative transposon Tn5251 of Streptococcus pneumoniae. FEMS Microbiol Lett 135, 231–236.[CrossRef]
    [Google Scholar]
  17. Reichmann, P., Varon, E., Günther, E., Reinert, R. R., Lütticken, R., Marton, A., Geslin, P., Wagner, J. & Hakenbeck, R. ( 1995;). Penicillin-resistant Streptococcus pneumoniae in Germany: genetic relationship to clones from other European countries. J Med Microbiol 43, 377–385.[CrossRef]
    [Google Scholar]
  18. Reinert, R. R., Kaufhold, A. & Kierdorf, H. ( 1992;). Penicillin-resistant pneumococcus in community-acquired bacteremic pneumonia in Germany. Infection 20, 238–239.[CrossRef]
    [Google Scholar]
  19. Reinert, R. R., Queck, A., Kaufhold, A., Kresken, M. & Lütticken, R. ( 1995;). Antimicrobial resistance and type distribution of Streptococcus pneumoniae isolates causing systemic infections in Germany, 1992–1994. Clin Infect Dis 21, 1398–1401.[CrossRef]
    [Google Scholar]
  20. Reinert, R. R., Simic, S., Al-Lahham, A., Reinert, S., Lemperle, M. & Lütticken, R. ( 2001;). Antimicrobial resistance of Streptococcus pneumoniae recovered from outpatients with respiratory tract infections in Germany from 1998 to 1999: results of a national surveillance study. J Clin Microbiol 39, 1187–1189.[CrossRef]
    [Google Scholar]
  21. Reinert, R. R., Al-Lahham, A., Lemperle, M., Tenholte, C., Briefs, C., Haupts, S., Gerards, H. H. & Lütticken, R. ( 2002;). Emergence of macrolide and penicillin resistance among invasive pneumococcal isolates in Germany. J Antimicrob Chemother 49, 61–68.[CrossRef]
    [Google Scholar]
  22. Shi, Z.-Y., Enright, M. C., Wilkinson, P., Griffiths, D. & Spratt, B. G. ( 1998;). Identification of three major clones of multiply antibiotic-resistant Streptococcus pneumoniae in Taiwanese hospitals by multilocus sequence typing. J Clin Microbiol 36, 3514–3519.
    [Google Scholar]
  23. Tait-Kamradt, A., Clancy, J., Cronan, M., Dib-Hajj, F., Wondrack, L., Yuan, W. & Sutcliffe, J. ( 1997;). mefE is necessary for the erythromycin-resistant M phenotype in Streptococcus pneumoniae. Antimicrob Agents Chemother 41, 2251–2255.
    [Google Scholar]
  24. Trieu-Cuot, P., Poyart-Salmeron, C., Carlier, C. & Courvalin, P. ( 1990;). Nucleotide sequence of the erythromycin resistance gene of the conjugative transposon Tn1545. Nucleic Acids Res 18, 3660. 3660.[CrossRef]
    [Google Scholar]
  25. von Kries, R., Siedler, A., Schmitt, H. J. & Reinert, R. R. ( 2000;). Proportion of invasive pneumococcal infections in German children preventable by pneumococcal conjugate vaccines. Clin Infect Dis 31, 482–487.[CrossRef]
    [Google Scholar]
  26. Widdowson, C. A. & Klugman, K. P. ( 1998;). The molecular mechanisms of tetracycline resistance in the pneumococcus. Microb Drug Resist 4, 79–84.[CrossRef]
    [Google Scholar]
  27. Widdowson, C. A., Klugman, K. P. & Hanslo, D. ( 1996;). Identification of the tetracycline resistance gene, tet(O), in Streptococcus pneumoniae. Antimicrob Agents Chemother 40, 2891–2893.
    [Google Scholar]
  28. Zhou, J., Enright, M. C. & Spratt, B. G. ( 2000;). Identification of the major Spanish clones of penicillin-resistant pneumococci via the Internet using multilocus sequence typing. J Clin Microbiol 38, 977–986.
    [Google Scholar]
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