1887

Abstract

The suitability of a PCR procedure using a pair of primers targeting the gene was evaluated as a means of detecting species. A total of 33 strains from 27 serovars and 15 non- strains from eight different genera were included. PCR with all the strains produced a 784 bp DNA fragment that was absent from all the non- strains tested. The detection limit of the PCR was 100 pg with genomic DNA and 3 × 10 c.f.u. ml with serial dilutions of bacterial culture. An enrichment-PCR method was further developed to test the sensitivity of the primers for the detection of in faecal samples spiked with different concentrations of subsp. serovar Typhimurium. The method described allowed the detection of Typhimurium in faecal samples at a concentration of 3 × 10 c.f.u. ml. In conclusion, the primers are specific for species and the PCR method presented may be suitable for the detection of in faeces.

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2003-09-01
2019-11-20
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