1887

Abstract

Avian intestinal spirochaetosis (AIS) results from the colonization of the caeca and colon of poultry by pathogenic , notably . Following the ban on the use of antibiotic growth promoters in the European Union in 2006, the number of cases of AIS has increased, which, alongside emerging antimicrobial resistance in , has driven renewed interest in alternative intervention strategies. -based probiotics have been shown to protect against infection with common enteric pathogens in livestock. Our previous studies have shown that LM1 antagonizes aspects of the pathobiology of . Here, we showed that LM1 mitigates the clinical symptoms of AIS in chickens experimentally challenged with . Two groups of 15 commercial laying hens were challenged experimentally by oral gavage with B2904 at 18 weeks of age; one group received unsupplemented drinking water and the other received LM1 in drinking water from 1 week prior to challenge with and thereafter for the duration of the study. This treatment regime was protective. Specifically, was detected by culture in fewer birds, bird weights were higher, faecal moisture contents were significantly lower (<0.05) and egg production as assessed by egg weight and faecal staining score was improved (<0.05). Also, at post-mortem examination, significantly fewer were recovered from treated birds (<0.05), with only mild–moderate histopathological changes observed. These data suggest that LM1 may be a useful tool in the control of AIS.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.051862-0
2013-02-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/62/2/287.html?itemId=/content/journal/jmm/10.1099/jmm.0.051862-0&mimeType=html&fmt=ahah

References

  1. Abbas Hilmi H. T. , Surakka A. , Apajalahti J. , Saris P. E. . ( 2007; ). Identification of the most abundant Lactobacillus species in the crop of 1- and 5-week-old broiler chickens. . Appl Environ Microbiol 73:, 7867–7873. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bait-Merabet L. , Thille A. , Legrand P. , Brun-Buisson C. , Cattoir V. . ( 2008; ). Brachyspira pilosicoli bloodstream infections: case report and review of the literature. . Ann Clin Microbiol Antimicrob 7:, 19. [CrossRef] [PubMed]
    [Google Scholar]
  3. Balevi T. , Uçan U. S. , Coşkun B. , Kurtoglu V. , Cetingül I. S. . ( 2001; ). Effect of dietary probiotic on performance and humoral immune response in layer hens. . Br Poult Sci 42:, 456–461. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bernardeau M. , Gueguen M. , Smith D. G. , Corona-Barrera E. , Vernoux J. P. . ( 2009; ). In vitro antagonistic activities of Lactobacillus spp. against Brachyspira hyodysenteriae and Brachyspira pilosicoli . . Vet Microbiol 138:, 184–190. [CrossRef] [PubMed]
    [Google Scholar]
  5. Burch D. G. S. . ( 2010; ). Egg production drops from Brachyspira . . World Polit 26:, 24–25.
    [Google Scholar]
  6. Castanon J. I. . ( 2007; ). History of the use of antibiotic as growth promoters in European poultry feeds. . Poult Sci 86:, 2466–2471. [CrossRef] [PubMed]
    [Google Scholar]
  7. Collins J. W. , La Ragione R. M. , Woodward M. J. , Searle L. E. J. . ( 2009; ). Application of prebiotics and probiotics in livestock. . In Prebiotics and Probiotics Science and Technology, pp. 1123–1192. Edited by Charalampopoulos D. , Rastall R. A. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  8. Davelaar F. G. , Smit H. F. , Hovind-Hougen K. , Dwars R. M. , Vandervalk P. C. . ( 1986; ). Infectious typhlitis in chickens caused by spirochetes. . Avian Pathol 15:, 247–258. [CrossRef] [PubMed]
    [Google Scholar]
  9. De Man J. C. , Rogosa M. , Sharpe M. E. . ( 1960; ). A medium for the cultivation of lactobacilli. . J Appl Microbiol 23:, 130–135. [CrossRef]
    [Google Scholar]
  10. Edens F. W. , Parkhurst C. R. , Casas I. A. , Dobrogosz W. J. . ( 1997; ). Principles of ex ovo competitive exclusion and in ovo administration of Lactobacillus reuteri . . Poult Sci 76:, 179–196.[PubMed] [CrossRef]
    [Google Scholar]
  11. FAO/WHO. ( 2001; ). Health and Nutritional Properties of Probiotics of Food Including Powder Milk with Live Lactic Acid Bacteria . . Report of a joint FAO/WHO expert consultation.
  12. Feberwee A. , Hampson D. J. , Phillips N. D. , La T. , van der Heijden H. M. , Wellenberg G. J. , Dwars R. M. , Landman W. J. . ( 2008; ). Identification of Brachyspira hyodysenteriae and other pathogenic Brachyspira species in chickens from laying flocks with diarrhea or reduced production or both. . J Clin Microbiol 46:, 593–600. [CrossRef] [PubMed]
    [Google Scholar]
  13. Fellström C. , Pettersson B. , Uhlén M. , Gunnarsson A. , Johansson K. E. . ( 1995; ). Phylogeny of Serpulina based on sequence analyses of the 16S rRNA gene and comparison with a scheme involving biochemical classification. . Res Vet Sci 59:, 5–9. [CrossRef] [PubMed]
    [Google Scholar]
  14. Fellström C. , Pettersson B. , Thomson J. , Gunnarsson A. , Persson M. , Johansson K. E. . ( 1997; ). Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR. . J Clin Microbiol 35:, 462–467.[PubMed]
    [Google Scholar]
  15. Glávits R. , Ivanics E. , Thuma A. , Kaszanyitzky E. , Samu P. , Ursu K. , Dencso L. , Dán A. . ( 2011; ). Typhlocolitis associated with spirochaetes in duck flocks. . Avian Pathol 40:, 23–31. [CrossRef] [PubMed]
    [Google Scholar]
  16. Grashorn M. A. , Simonovic S. . ( 2009; ). Wholesome drinking water to prevent watery eggs. . World Polit 25:, 18–19.
    [Google Scholar]
  17. Griffiths I. B. , Hunt B. W. , Lister S. A. , Lamont M. H. . ( 1987; ). Retarded growth rate and delayed onset of egg production associated with spirochaete infection in pullets. . Vet Rec 121:, 35–37. [CrossRef] [PubMed]
    [Google Scholar]
  18. Hampson D. J. , McLaren A. J. . ( 1999; ). Experimental infection of laying hens with Serpulina intermedia causes reduced egg production and increased faecal water content. . Avian Pathol 28:, 113–117. [CrossRef]
    [Google Scholar]
  19. Hampson D. J. , Oxberry S. L. , La T. . ( 2006a; ). Potential for zoonotic transmission of Brachyspira pilosicoli . . Emerg Infect Dis 12:, 869–870. [CrossRef] [PubMed]
    [Google Scholar]
  20. Hampson D. J. , Stephens C. P. , Oxberry S. L. . ( 2006b; ). Antimicrobial susceptibility testing of Brachyspira intermedia and Brachyspira pilosicoli isolates from Australian chickens. . Avian Pathol 35:, 12–16. [CrossRef] [PubMed]
    [Google Scholar]
  21. Higgins S. E. , Higgins J. P. , Wolfenden A. D. , Henderson S. N. , Torres-Rodriguez A. , Tellez G. , Hargis B. . ( 2008; ). Evaluation of a Lactobacillus-based probiotic culture for the reduction of Salmonella enteritidis in neonatal broiler chicks. . Poult Sci 87:, 27–31. [CrossRef] [PubMed]
    [Google Scholar]
  22. Jamshidi A. , Hampson D. J. . ( 2003; ). Experimental infection of layer hens with a human isolate of Brachyspira pilosicoli . . J Med Microbiol 52:, 361–364. [CrossRef] [PubMed]
    [Google Scholar]
  23. Jensen T. K. , Møller K. , Boye M. , Leser T. D. , Jorsal S. E. . ( 2000; ). Scanning electron microscopy and fluorescent in situ hybridization of experimental Brachyspira (Serpulina) pilosicoli infection in growing pigs. . Vet Pathol 37:, 22–32. [CrossRef] [PubMed]
    [Google Scholar]
  24. Jensen T. K. , Boye M. , Ahrens P. , Korsager B. , Teglbjaerg P. S. , Lindboe C. F. , Møller K. . ( 2001; ). Diagnostic examination of human intestinal spirochetosis by fluorescent in situ hybridization for Brachyspira aalborgi, Brachyspira pilosicoli, and other species of the genus Brachyspira (Serpulina). . J Clin Microbiol 39:, 4111–4118. [CrossRef] [PubMed]
    [Google Scholar]
  25. Kostman J. R. , Patel M. , Catalano E. , Camacho J. , Hoffpauir J. , DiNubile M. J. . ( 1995; ). Invasive colitis and hepatitis due to previously uncharacterized spirochetes in patients with advanced human immunodeficiency virus infection. . Clin Infect Dis 21:, 1159–1165. [CrossRef] [PubMed]
    [Google Scholar]
  26. Kurtoglu V. , Kurtoglu F. , Seker E. , Coskun B. , Balevi T. , Polat E. S. . ( 2004; ). Effect of probiotic supplementation on laying hen diets on yield performance and serum and egg yolk cholesterol. . Food Addit Contam 21:, 817–823. [CrossRef] [PubMed]
    [Google Scholar]
  27. Kwon H. S. , Yang E. H. , Yeon S. W. , Kang B. H. , Kim T. Y. . ( 2004; ). Rapid identification of probiotic Lactobacillus species by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA. . FEMS Microbiol Lett 239:, 267–275. [CrossRef] [PubMed]
    [Google Scholar]
  28. La Ragione R. M. , Woodward M. J. . ( 2003; ). Competitive exclusion by Bacillus subtilis spores of Salmonella enterica serotype Enteritidis and Clostridium perfringens in young chickens. . Vet Microbiol 94:, 245–256. [CrossRef] [PubMed]
    [Google Scholar]
  29. La Ragione R. M. , Narbad A. , Gasson M. J. , Woodward M. J. . ( 2004; ). In vivo characterization of Lactobacillus johnsonii FI9785 for use as a defined competitive exclusion agent against bacterial pathogens in poultry. . Lett Appl Microbiol 38:, 197–205. [CrossRef] [PubMed]
    [Google Scholar]
  30. Lan P. T. , Binh T. , Benno Y. . ( 2003; ). Impact of two probiotic Lactobacillus strains feeding on fecal lactobacilli and weight gains in chicken. . J Gen Appl Microbiol 49:, 29–36. [CrossRef] [PubMed]
    [Google Scholar]
  31. Mappley L. J. , Tchórzewska M. A. , Cooley W. A. , Woodward M. J. , La Ragione R. M. . ( 2011; ). Lactobacilli antagonize the growth, motility, and adherence of Brachyspira pilosicoli: a potential intervention against avian intestinal spirochetosis. . Appl Environ Microbiol 77:, 5402–5411. [CrossRef] [PubMed]
    [Google Scholar]
  32. Mikosza A. S. , La T. , Margawani K. R. , Brooke C. J. , Hampson D. J. . ( 2001; ). PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces. . FEMS Microbiol Lett 197:, 167–170. [CrossRef] [PubMed]
    [Google Scholar]
  33. Nahanshon S. N. , Nakaue H. S. , Mirosh L. W. . ( 1992; ). Effect of direct-fed microbials on nutrient retention and parameters of laying pullets. . Poult Sci 71:, 111.
    [Google Scholar]
  34. Nahanshon S. N. , Nakaue H. S. , Mirosh L. W. . ( 1994; ). Production variables and nutrient retention in single comb white leghorn laying pullets fed diets supplemented with direct-fed microbials. . Poult Sci 73:, 1699–1711. [CrossRef] [PubMed]
    [Google Scholar]
  35. Phillips N. D. , La T. , Hampson D. J. . ( 2005; ). A cross-sectional study to investigate the occurrence and distribution of intestinal spirochaetes (Brachyspira spp.) in three flocks of laying hens. . Vet Microbiol 105:, 189–198. [CrossRef] [PubMed]
    [Google Scholar]
  36. Prim N. , Pericas R. , Español M. , Rivera A. , Mirelis B. , Coll P. . ( 2011; ). Bloodstream infection due to Brachyspira pilosicoli in a patient with multiorgan failure. . J Clin Microbiol 49:, 3697–3699. [CrossRef] [PubMed]
    [Google Scholar]
  37. Pringle M. , Landén A. , Franklin A. . ( 2006; ). Tiamulin resistance in porcine Brachyspira pilosicoli isolates. . Res Vet Sci 80:, 1–4. [CrossRef] [PubMed]
    [Google Scholar]
  38. Råsbäck T. , Fellström C. , Bergsjø B. , Cizek A. , Collin K. , Gunnarsson A. , Jensen S. M. , Mars A. , Thomson J. . & other authors ( 2005; ). Assessment of diagnostics and antimicrobial susceptibility testing of Brachyspira species using a ring test. . Vet Microbiol 109:, 229–243. [CrossRef] [PubMed]
    [Google Scholar]
  39. Schoeni J. L. , Wong A. C. . ( 1994; ). Inhibition of Campylobacter jejuni colonization in chicks by defined competitive exclusion bacteria. . Appl Environ Microbiol 60:, 1191–1197.[PubMed]
    [Google Scholar]
  40. Se C. Y. , Yong F. F. , Tan H. M. , Yeo W. M. , Teo inventors A. Y. L. . . ( 2008; ). Broad-Spectrum Antibacterial and Antifungal Activity of Lactobacillus Johnsonii D115. United States patent.</bold>
  41. Stanton T. B. , Postic D. , Jensen N. S. . ( 1998; ). Serpulina alvinipulli sp. nov., a new Serpulina species that is enteropathogenic for chickens. . Int J Syst Bacteriol 48:, 669–676. [CrossRef] [PubMed]
    [Google Scholar]
  42. Stephens C. P. , Hampson D. J. . ( 2001; ). Intestinal spirochete infections of chickens: a review of disease associations, epidemiology and control. . Anim Health Res Rev 2:, 83–91.[PubMed]
    [Google Scholar]
  43. Stephens C. P. , Hampson D. J. . ( 2002a; ). Evaluation of tiamulin and lincomycin for the treatment of broiler breeders experimentally infected with the intestinal spirochaete Brachyspira pilosicoli . . Avian Pathol 31:, 299–304. [CrossRef] [PubMed]
    [Google Scholar]
  44. Stephens C. P. , Hampson D. J. . ( 2002b; ). Experimental infection of broiler breeder hens with the intestinal spirochaete Brachyspira (Serpulina) pilosicoli causes reduced egg production. . Avian Pathol 31:, 169–175. [CrossRef] [PubMed]
    [Google Scholar]
  45. Stern N. J. , Svetoch E. A. , Eruslanov B. V. , Perelygin V. V. , Mitsevich E. V. , Mitsevich I. P. , Pokhilenko V. D. , Levchuk V. P. , Svetoch O. E. , Seal B. S. . ( 2006; ). Isolation of a Lactobacillus salivarius strain and purification of its bacteriocin, which is inhibitory to Campylobacter jejuni in the chicken gastrointestinal system. . Antimicrob Agents Chemother 50:, 3111–3116. [CrossRef] [PubMed]
    [Google Scholar]
  46. Swayne D. E. , McLaren A. J. . ( 1997; ). Avian intestinal spirochaetes and avian intestinal spirochaetosis. . In Intestinal Spirochaetes in Domestic Animals and Humans, pp. 267–300. Edited by Hampson D. J. , Stanton T. B. . . Wallingford, UK:: CAB International;.
    [Google Scholar]
  47. Swayne D. E. , Bermudez A. J. , Sagartz J. E. , Eaton K. A. , Monfort J. D. , Stoutenburg J. W. , Hayes J. R. . ( 1992; ). Association of cecal spirochetes with pasty vents and dirty eggshells in layers. . Avian Dis 36:, 776–781. [CrossRef] [PubMed]
    [Google Scholar]
  48. Timmerman H. M. , Veldman A. , van den Elsen E. , Rombouts F. M. , Beynen A. C. . ( 2006; ). Mortality and growth performance of broilers given drinking water supplemented with chicken-specific probiotics. . Poult Sci 85:, 1383–1388.[PubMed] [CrossRef]
    [Google Scholar]
  49. Tortuero F. , Fernandez E. . ( 1995; ). Effects of inclusion of microbial cultures in barley-based diets fed to laying hens. . Anim Feed Sci Technol 53:, 255–265. [CrossRef]
    [Google Scholar]
  50. Trott D. J. , Stanton T. B. , Jensen N. S. , Duhamel G. E. , Johnson J. L. , Hampson D. J. . ( 1996; ). Serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. . Int J Syst Bacteriol 46:, 206–215. [CrossRef] [PubMed]
    [Google Scholar]
  51. Tsinganou E. , Gebbers J. O. . ( 2010; ). Human intestinal spirochetosis – a review. . Ger Med Sci 8:, Doc01.[PubMed]
    [Google Scholar]
  52. Vanderpool C. , Yan F. , Polk D. B. . ( 2008; ). Mechanisms of probiotic action: implications for therapeutic applications in inflammatory bowel diseases. . Inflamm Bowel Dis 14:, 1585–1596. [CrossRef] [PubMed]
    [Google Scholar]
  53. Vicente J. L. , Torres-Rodriguez A. , Higgins S. E. , Pixley C. , Tellez G. , Donoghue A. M. , Hargis B. M. . ( 2008; ). Effect of a selected Lactobacillus spp.-based probiotic on Salmonella enterica serovar Enteritidis-infected broiler chicks. . Avian Dis 52:, 143–146. [CrossRef] [PubMed]
    [Google Scholar]
  54. Zhang G. , Ma L. , Doyle M. P. . ( 2007a; ). Salmonellae reduction in poultry by competitive exclusion bacteria Lactobacillus salivarius and Streptococcus cristatus . . J Food Prot 70:, 874–878.[PubMed]
    [Google Scholar]
  55. Zhang G. , Ma L. , Doyle M. P. . ( 2007b; ). Potential competitive exclusion bacteria from poultry inhibitory to Campylobacter jejuni and Salmonella . . J Food Prot 70:, 867–873.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.051862-0
Loading
/content/journal/jmm/10.1099/jmm.0.051862-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error