1887

Abstract

subsp serovar Typhimurium (. Typhimurium) can initiate entry into non-phagocytic epithelial cells by triggering certain signal transduction pathways, thereby allowing the pathogen to invade and establish a niche within host cells. Anaerobiosis has been shown to be an important inducer of the invasion process of . Typhimurium. However, the effect of anaerobiosis on modulation of cell signalling cascades by . Typhimurium is not known. In the present study, the phospholipase Cγ signalling cascade was investigated in mice enterocytes, following interaction with . Typhimurium grown under aerobic and anaerobic growth conditions. Significant increases in enterocyte intracellular calcium and inositol 1,4,5-triphosphate levels were observed on interaction with . Typhimurium grown anaerobically compared with . Typhimurium grown aerobically. An increased membrane/cytosolic ratio of protein kinase C was also seen with anaerobic . Typhimurium in enterocytes compared with aerobic . Typhimurium. These data suggest that anaerobically grown organisms are more efficient in initiating cell-signalling events than are aerobically grown bacteria. These enhanced cell signals may contribute to the increased virulence of . Typhimurium grown anaerobically.

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2003-09-01
2020-09-26
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