1887

Abstract

A gene cluster encoding enzymes involved in LPS O antigen biosynthesis was identified from the partial genome sequence of subsp. Schu S4. All of the genes within the cluster were assigned putative functions based on sequence similarity with genes from O antigen biosynthetic clusters from other bacteria. Ten pairs of overlapping primers were designed to amplify the O antigen biosynthetic cluster by PCR from nine strains of . Although the gene cluster was present in all strains, there was a size difference in one of the PCR products between subsp. strains and subsp. strains. LPS was purified from subsp. Schu S4 and the O antigen was shown by mass spectrometry to have a structure similar to that of subsp. strain 15. When LPS from subsp. Schu S4 was used to immunize mice that were then challenged with subsp. Schu S4, an extended time to death was observed.

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2003-10-01
2019-10-15
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