1887

Abstract

Formation of infectious urinary calculi is the most common complication accompanying urinary tract infections by members of the genus . The major factor involved in stone formation is the urease produced by these bacteria, which causes local supersaturation and crystallization of magnesium and calcium phosphates as carbonate apatite [Ca(PO).CO] and struvite (MgNHPO.6HO), respectively. This effect may also be enhanced by bacterial polysaccharides. Macromolecules of such kind contain negatively charged residues that are able to bind Ca and Mg, leading to the accumulation of these ions around bacterial cells and acceleration of the crystallization process. The levels of Ca and Mg ions bound by whole cells were measured, as well as the chemical nature of isolated LPS polysaccharides, and the intensity of the crystallization process was compared in a synthetic urine. The results suggest that the sugar composition of LPS may either enhance or inhibit the crystallization of struvite and apatite, depending on its chemical structure and ability to bind cations. This points to the increased importance of endotoxin in urinary tract infections.

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2003-06-01
2019-11-12
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