1887

Abstract

Lactic acid-producing lactobacilli were selected from 134 human vaginal isolates by testing their capability to inhibit the growth of different pathogenic micro-organisms. CRL 1259 (from the CERELA Culture Collection) was selected to study the effects of temperature, pH and culture medium on growth and lactic acid production. Growth parameters were estimated by using the model of Gompertz. Kinetics of inhibition of uropathogenic were evaluated in mixed cultures of the pathogen and . Optimal conditions for growth and lactic acid production by were pH 6.5 or 8.0 and 37 °C. Under these conditions, growth was higher in LAPTg (yeast extract/peptone/tryptone/Tween 80/glucose) broth than in MRS (De Man–Rogosa–Sharpe) broth. However, lactic acid production was more efficient in MRS broth. Under optimal conditions for lactic acid production, inhibited the growth of . These results suggest that inclusion of CRL 1259 in probiotic products for vaginal application would be beneficial.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05155-0
2003-12-01
2019-11-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/52/12/JM521212.html?itemId=/content/journal/jmm/10.1099/jmm.0.05155-0&mimeType=html&fmt=ahah

References

  1. Andersch, B., Forssman, L., Lincoln, K. & Tortensson, P. ( 1986;). Treatment of bacterial vaginosis with an acid cream: a comparison between the effect of lactate-gel and metronidazole. Gynecol Obstet Invest 21, 19–25.[CrossRef]
    [Google Scholar]
  2. Aroutcheva, A., Gariti, D., Simon, M., Shott, S., Faro, J., Simoes, J. A., Gurguis, A. & Faro, S. ( 2001;). Defense factors of vaginal lactobacilli. Am J Obstet Gynecol 185, 375–379.[CrossRef]
    [Google Scholar]
  3. Boskey, E. R., Telsch, K. M., Whaley, K. J., Moench, T. R. & Cone, R. A. ( 1999;). Acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidification. Infect Immun 67, 5170–5175.
    [Google Scholar]
  4. Boskey, E. R., Cone, R. A., Whaley, K. J. & Moench, T. R. ( 2001;). Origins of vaginal acidity: high d/l lactate ratio is consistent with bacteria being the primary source. Hum Reprod 16, 1809–1813.[CrossRef]
    [Google Scholar]
  5. De Man, J. C., Rogosa, M. & Sharpe, M. E. ( 1960;). A medium for the cultivation of lactobacilli. J Appl Bacteriol 23, 130–135.[CrossRef]
    [Google Scholar]
  6. Efron, B. ( 1982;). The bootstrap. In The Jackknife, the Bootstrap, and Other Resampling Plans, pp. 27–36. Edited by B. Efron. Philadelphia, PA: Society for Industrial and Applied Mathematics.
  7. Eschenbach, D. A. ( 1993;). History and review of bacterial vaginosis. Am J Obstet Gynecol 169, 441–445.[CrossRef]
    [Google Scholar]
  8. Geshnizgani, A. M. & Onderdock, A. B. ( 1992;). Defined medium simulating genital tract secretions for growth of vaginal microflora. J Clin Microbiol 30, 1323–1326.
    [Google Scholar]
  9. Hanna, N. F., Taylor-Robinson, D., Kalodiki-Karamanoli, M., Harris, J. R. & McFadyen, I. R. ( 1985;). The relation between vaginal pH and the microbiological status in vaginitis. Br J Obstet Gynaecol 92, 1267–1271.[CrossRef]
    [Google Scholar]
  10. Huet, S., Bouvier, A., Gruet, M.-A. & Jolivet, E. ( 1996;). Accuracy of estimators, confidence intervals and tests. In Statistical Tools for Nonlinear Regression, pp. 29–59. New York: Springer.
  11. Jack, R. W., Tagg, J. R. & Ray, B. ( 1995;). Bacteriocins of Gram-positive bacteria. Microbiol Rev 59, 171–200.
    [Google Scholar]
  12. Juárez Tomás, M. S., Bru de Labanda, E., de Ruiz Holgado, A. P. & Nader-Macías, M. E. ( 2002a;). Estimation of vaginal probiotic lactobacilli growth parameters with the application of the Gompertz model. Can J Microbiol 48, 82–92.[CrossRef]
    [Google Scholar]
  13. Juárez Tomás, M. S., Bru, E., Wiese, B., de Ruiz Holgado, A. A. P. & Nader-Macías, M. E. ( 2002b;). Influence of pH, temperature and culture media on the growth and bacteriocin production by vaginal Lactobacillus salivarius CRL 1328. J Appl Microbiol 93, 714–724.[CrossRef]
    [Google Scholar]
  14. Kotarski, S. F. & Savage, D. C. ( 1979;). Models for study of the specificity by which indigenous lactobacilli adhere to murine gastric epithelia. Infect Immun 26, 966–975.
    [Google Scholar]
  15. Kylä-Nikkilä, K., Hujanen, M., Leisola, M. & Palva, A. ( 2000;). Metabolic engineering of Lactobacillus helveticus CNRZ32 for production of pure l-(+)-lactic acid. Appl Environ Microbiol 66, 3835–3841.[CrossRef]
    [Google Scholar]
  16. Lindstrom, M. J. & Bates, D. M. ( 1990;). Nonlinear mixed effects models for repeated measures data. Biometrics 46, 673–687.[CrossRef]
    [Google Scholar]
  17. McLean, N. W. & Rosenstein, I. J. ( 2000;). Characterisation and selection of a Lactobacillus species to re-colonise the vagina of women with recurrent bacterial vaginosis. J Med Microbiol 49, 543–552.
    [Google Scholar]
  18. Melis, G. B., Ibba, M. T., Steri, B., Kotsonis, P., Matta, V. & Paoletti, A. M. ( 2000;). Role of pH as a regulator of vaginal physiological environment. Minerva Ginecol 52, 111–121 (in Italian).
    [Google Scholar]
  19. Nader de Macías, M. E., de Ruiz, C. S., López de Bocanera, M. E. & Pesce de Ruiz Holgado, A. A. ( 1996;). Preventive and therapeutic effects of lactobacilli on urinary tract infections in mice. Anaerobe 2, 85–93.[CrossRef]
    [Google Scholar]
  20. Ocaña, V. S., Bru, E., de Ruiz Holgado, A. A. & Nader-Macías, M. E. ( 1999a;). Surface characteristics of lactobacilli isolated from human vagina. J Gen Appl Microbiol 45, 203–212.[CrossRef]
    [Google Scholar]
  21. Ocaña, V. S., Pesce de Ruiz Holgado, A. A. & Nader-Macías, M. E. ( 1999b;). Selection of vaginal H2O2-generating Lactobacillus species for probiotic use. Curr Microbiol 38, 279–284.[CrossRef]
    [Google Scholar]
  22. Ocaña, V. S., de Ruiz Holgado, A. A. & Nader-Macías, M. E. ( 1999c;). Growth inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus paracasei subsp.paracasei isolated from the human vagina. FEMS Immunol Med Microbiol 23, 87–92.[CrossRef]
    [Google Scholar]
  23. Ocaña, V. S., Pesce de Ruiz Holgado, A. A. & Nader-Macías, M. E. ( 1999d;). Characterization of a bacteriocin-like substance produced by a vaginal Lactobacillus salivarius strain. Appl Environ Microbiol 65, 5631–5635.
    [Google Scholar]
  24. Paavonen, J. ( 1983;). Physiology and ecology of the vagina. Scand J Infect Dis Suppl 40, 31–35.
    [Google Scholar]
  25. Passos, F. V., Fleming, H. P., Ollis, D. F., Felder, R. M. & McFeeters, R. F. ( 1994;). Kinetics and modeling of lactic acid production by Lactobacillus plantarum. Appl Environ Microbiol 60, 2627–2636.
    [Google Scholar]
  26. Raibaud, P., Galpin, J. V., Ducluzeau, R., Mocquot, G. & Oliver, G. ( 1973;). Le genre Lactobacillus dans le tube digestif du rat.II. Caractères de souches heterofermentaires isolées de rats ‘‘Holo’’ et ‘‘Gnotoxéniques’'. Ann Inst Pasteur Microbiol 124A, 2223–2235 (in French).
    [Google Scholar]
  27. Redondo-López, V., Cook, R. L. & Sobel, J. D. ( 1990;). Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora. Rev Infect Dis 12, 856–872.[CrossRef]
    [Google Scholar]
  28. Reid, G., Chan, R. C., Bruce, A. W. & Costerton, J. W. ( 1985;). Prevention of urinary tract infection in rats with an indigenous Lactobacillus casei strain. Infect Immun 49, 320–324.
    [Google Scholar]
  29. Reid, G., Bruce, A. W. & Taylor, M. ( 1992;). Influence of three-day antimicrobial therapy and Lactobacillus vaginal suppositories on recurrence of urinary tract infections. Clin Ther 14, 11–16.
    [Google Scholar]
  30. Silva-Ruiz, C., Nader-Macias, M. E., Lopez-Bocanera, M. E. & Pesce-Ruiz Holgado, A. ( 1993;). Lactobacillus fermentum administered in suspensions and in agarose beads to mice: a comparative study. Microbiol Alim Nutr 11, 391–397.
    [Google Scholar]
  31. Silva-Ruiz, C., Lopez-Bocanera, M. E., Nader-Macias, M. E. & Pesce-Ruiz Holgado, A. ( 1996;). Effect of lactobacilli and antibiotics on E.coli urinary infections in mice. Biol Pharm Bull 19, 88–93.[CrossRef]
    [Google Scholar]
  32. Silva-Ruiz, C., Rey, M. R., de Ruiz Holgado, A. P. & Nader-Macias, M. E. ( 2001;). Experimental administration of estradiol on the colonization of Lactobacillus fermentum and Escherichia coli in the urogenital tract of mice. Biol Pharm Bull 24, 127–134.[CrossRef]
    [Google Scholar]
  33. Stamey, T. A. & Kaufman, M. F. ( 1975;). Studies of introital colonization in women with recurrent urinary infections.II. A comparison of growth in normal vaginal fluid of common versus uncommom serogroups of Escherichia coli. J Urol 114, 264–267.
    [Google Scholar]
  34. Stamey, T. A. & Timothy, M. M. ( 1975;). Studies of introital colonization in women with recurrent urinary infections.I. The role of vaginal pH. J Urol 114, 261–263.
    [Google Scholar]
  35. Strus, M., Malinowska, M. & Heczko, P. B. ( 2002;). In vitro antagonistic effect of Lactobacillus on organisms associated with bacterial vaginosis. J Reprod Med 47, 41–46.
    [Google Scholar]
  36. Tevi-Bénissan, C., Bélec, L., Lévy, M., Schneider-Fauveau, V., Si Mohamed, A., Hallouin, M.-C., Matta, M. & Grésenguet, G. ( 1997;). In vivo semen-associated pH neutralization of cervicovaginal secretions. Clin Diagn Lab Immunol 4, 367–374.
    [Google Scholar]
  37. Zwietering, M. H., Jongenburger, I., Rombouts, F. M. & Van't Riet, K. ( 1990;). Modeling of the bacterial growth curve. Appl Environ Microbiol 56, 1875–1881.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05155-0
Loading
/content/journal/jmm/10.1099/jmm.0.05155-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error