1887

Abstract

Lactic acid-producing lactobacilli were selected from 134 human vaginal isolates by testing their capability to inhibit the growth of different pathogenic micro-organisms. CRL 1259 (from the CERELA Culture Collection) was selected to study the effects of temperature, pH and culture medium on growth and lactic acid production. Growth parameters were estimated by using the model of Gompertz. Kinetics of inhibition of uropathogenic were evaluated in mixed cultures of the pathogen and . Optimal conditions for growth and lactic acid production by were pH 6.5 or 8.0 and 37 °C. Under these conditions, growth was higher in LAPTg (yeast extract/peptone/tryptone/Tween 80/glucose) broth than in MRS (De Man–Rogosa–Sharpe) broth. However, lactic acid production was more efficient in MRS broth. Under optimal conditions for lactic acid production, inhibited the growth of . These results suggest that inclusion of CRL 1259 in probiotic products for vaginal application would be beneficial.

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2003-12-01
2024-03-28
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