1887

Abstract

in the human gut can be divided morphologically into spiral and coccoid forms. The spiral form is known to change into the coccoid form in culture . The ultrastructural changes and culturability of were studied in medium supplemented with different concentrations of glucose. ATCC 43504 was cultured in liquid medium containing 10 % heat-inactivated horse serum supplemented with glucose (at 0, 10, 100, 300 and 500 mM) for 7 days. Bacterial ultrastructure and culturability were examined daily. With extended time in culture, the spiral forms had transformed into coccoid forms in all media. The coccoid forms could be further divided into two types, A and B, by electron microscopy. The type A coccoid form had an irregular surface with few flagella and indistinct cytoplasmic membrane. The type B coccoid form had a better-maintained integral membrane structure and was the dominant form in 300 mM glucose-supplemented medium. The highest culturability was obtained using 300 mM glucose-supplemented medium. Based on observations of ultrastructural changes in relation to the culturability data, the coccoid forms could be categorized into three stages: dying, viable but non-culturable and proliferating organisms. The optimal glucose concentration for culture in this liquid medium culture experiment was approximately 300 mM.

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2003-08-01
2024-03-28
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References

  1. Asaka M, Kimura T, Kato M, Kudo M, Miki K, Ogoshi K, Kato T, Tatsuta M, Graham D. Y. 1994; Possible role of Helicobacter pylori infection in early gastric cancer development. Cancer 73:2691–2694 [CrossRef]
    [Google Scholar]
  2. Benaissa M, Babin P, Quellard N, Pezennec L, Cenatiempo Y, Fauchere J. L. 1996; Changes in Helicobacter pylori ultrastructure and antigens during conversion from the bacillary to the coccoid form. Infect Immun 64:2331–2335
    [Google Scholar]
  3. Bode G, Malfertheiner P, Ditschuneit H. 1988; Pathogenetic implications of ultrastructural findings in Campylobacter pylori related gastroduodenal disease. Scand J Gastroenterol Suppl 142:25–39
    [Google Scholar]
  4. Bode G, Mauch F, Malfertheiner P. 1993; The coccoid forms of Helicobacter pylori .Criteria for their viability. Epidemiol Infect 111:483–490 [CrossRef]
    [Google Scholar]
  5. Buck G. E, Gourley W. K, Lee W. K, Subramanyam K, Latimer J. M, DiNuzzo A. R. 1986; Relation of Campylobacter pyloridis to gastritis and peptic ulcer. J Infect Dis 153:664–669 [CrossRef]
    [Google Scholar]
  6. Catrenich C. E, Makin K. M. 1991; Characterization of the morphologic conversion of Helicobacter pylori from bacillary to coccoid forms. Scand J Gastroenterol Suppl 181:58–64
    [Google Scholar]
  7. Cellini L, Allocati N, Di Campli E, Dainelli B. 1994; Helicobacter pylori : a fickle germ. Microbiol Immunol 38:25–30 [CrossRef]
    [Google Scholar]
  8. Chalk P. A, Roberts A. D, Blows W. M. 1994; Metabolism of pyruvate and glucose by intact cells of Helicobacter pylori studied by 13C NMR spectroscopy. Microbiology 140:2085–2092 [CrossRef]
    [Google Scholar]
  9. Chan W. Y, Hui P. K, Leung K. M, Chow J, Kwok F, Ng C. S. 1994; Coccoid forms of Helicobacter pylori in the human stomach. Am J Clin Pathol 102:503–507
    [Google Scholar]
  10. Eaton K. A, Catrenich C. E, Makin K. M, Krakowka S. 1995; Virulence of coccoid and bacillary forms of Helicobacter pylori in gnotobiotic piglets. J Infect Dis 171:459–462 [CrossRef]
    [Google Scholar]
  11. Geis G, Leying H, Suerbaum S, Opferkuch W. 1990; Unusual fatty acid substitution in lipids and lipopolysaccharides of Helicobacter pylori . J Clin Microbiol 28:930–932
    [Google Scholar]
  12. Hua J, Ho B. 1996; Is the coccoid form of Helicobacter pylori viable?. Microbios 87:103–112
    [Google Scholar]
  13. Kaeberlein T, Lewis K, Epstein S. S. 2002; Isolating ‘‘uncultivable’’ microorganisms in pure culture in a simulated natural environment. Science 296:1127–1129 [CrossRef]
    [Google Scholar]
  14. Kusters J. G, Gerrits M. M, Van Strijp J. A, Vandenbroucke-Grauls C. M. 1997; Coccoid forms of Helicobacter pylori are the morphologic manifestation of cell death. Infect Immun 65:3672–3679
    [Google Scholar]
  15. Mendz G. L, Hazell S. L, Burns B. P. 1993; Glucose utilization and lactate production by Helicobacter pylori . J Gen Microbiol 139:3023–3028 [CrossRef]
    [Google Scholar]
  16. Mizoguchi H, Fujioka T, Kishi K, Nishizono A, Kodama R, Nasu M. 1998; Diversity in protein synthesis and viability of Helicobacter pylori coccoid forms in response to various stimuli. Infect Immun 66:5555–5560
    [Google Scholar]
  17. Saito N, Sato F, Kato M, Takeda H, Sugiyama T, Asaka M. 1998; Detection of coccoid Helicobacter pylori : light microscopical immunogold silver enhancing stain. Helicobacter 3:170–173
    [Google Scholar]
  18. Saito N, Konishi K, Sato F, Kato M, Takeda H, Sugiyama T, Asaka M. 2003; Plural transformation processes from spiral to coccoid Helicobacter pylori and its viability. J Infect 46:49–55 [CrossRef]
    [Google Scholar]
  19. Sato F. 2000; Helicobacter pylori in culture: an ultrastructural study. Hokkaido Igaku Zasshi 75:187–196
    [Google Scholar]
  20. Shahamat M, Mai U, Paszko-Kolva C, Kessel M, Colwell R. R. 1993; Use of autoradiography to assess viability of Helicobacter pylori in water. Appl Environ Microbiol 59:1231–1235
    [Google Scholar]
  21. Slomiany B. L, Slomiany A. 1992; Mechanism of Helicobacter pylori pathogenesis: focus on mucus. J Clin Gastroenterol 14:Suppl. 1S114–S121 [CrossRef]
    [Google Scholar]
  22. Sorberg M, Nilsson M, Hanberger H, Nilsson L. E. 1996; Morphologic conversion of Helicobacter pylori from bacillary to coccoid form. Eur J Clin Microbiol Infect Dis 15:216–219 [CrossRef]
    [Google Scholar]
  23. Takeuchi H, Shirai M, Akada J. K, Tsuda M, Nakazawa T. 1998; Nucleotide sequence and characterization of cdrA , a cell division-related gene of Helicobacter pylori . J Bacteriol 180:5263–5268
    [Google Scholar]
  24. Tomb J.-F, White O, Kerlavage A. R. 39 other authors 1997; The complete genome sequence of the gastric pathogen Helicobacter pylori . Nature 388:539–547 [CrossRef]
    [Google Scholar]
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