Cellular fatty acid analysis by GLC is widely used in the species identification of mycobacteria. Combining mycolic acid cleavage products with shorter cellular fatty acids increases the informative value of the analysis. A key has been created to aid in the identification of all currently known slowly growing environmental species. In this scheme, the species are classified into six categories, each characterized by a combination of fatty markers shared by those species. Within each category, individual species may be distinguished by the presence or absence of specific marker substances, such as methyl-branched fatty acids or secondary alcohols. This study also describes earlier unpublished GLC profiles of 14 rare, slowly growing, environmental mycobacteria, Mycobacterium asiaticum, Mycobacterium botniense, Mycobacterium branderi, Mycobacterium conspicuum, Mycobacterium cookii, Mycobacterium doricum, Mycobacterium heckeshornense, Mycobacterium heidelbergense, Mycobacterium hiberniae, Mycobacterium kubicae, Mycobacterium lentiflavum, Mycobacterium scrofulaceum, Mycobacterium triplex and Mycobacterium tusciae. Though no single identification technique alone, even sequencing of an entire single gene such as 16S rRNA, can identify all mycobacterial species accurately, GLC has proven to be both reliable and reproducible in the identification of slowly growing mycobacteria. In cases of earlier unknown species, it generates useful information that allows their further classification and may lead to the description of novel species.
BranderE,
JantzenE,
HuttunenR,
JulkunenA,
KatilaM.-L.1992; Characterization of a distinct group of slowly growing mycobacteria by biochemical tests and lipid analyses. J Clin Microbiol 30:1972–1975
CarbonaraS,
TortoliE,
CostaD.7 other authors2000; Disseminated Mycobacterium terrae infection in a patient with advanced human immunodeficiency virus disease. Clin Infect Dis 30:831–835[CrossRef]
ChouS,
ChedoreP,
HaddadA,
PaulN. R,
KasatiyaS.1996; Direct identification of Mycobacterium species in Bactec 7H12B medium by gas-liquid chromatography. J Clin Microbiol 34:1317–1320
ChouS,
ChedoreP,
KasatiyaS.1998; Use of gas chromatographic fatty acid and mycolic acid cleavage product determination to differentiate among Mycobacterium genavense , Mycobacterium fortuitum , Mycobacterium simiae , and Mycobacterium tuberculosis . J Clin Microbiol 36:577–579
Garcia-BarcelóM,
LuquinM,
BeldaF,
AusinaV.1993; Gas chromatographic whole-cell fatty acid analysis as an aid for the identification of mixed mycobacterial cultures. J Chromatogr 617:299–303[CrossRef]
Koukila-KähköläP,
PaulinL,
BranderE,
JantzenE,
Eho-RemesM,
KatilaM.-L.2000; Characterisation of a new isolate of Mycobacterium shimoidei from Finland. J Med Microbiol 49:937–940
LarssonL.1983; Acidic methanolysis v. alkaline saponification in gas chromatographic characterization of mycobacteria: differentiation between Mycobacterium avium – intracellulare and Mycobacterium gastri . Acta Pathol Microbiol Immunol Scand Sect B 91:235–239
LarssonL,
JiménezJ,
Valero-GuillénP,
Martín-LuengoF,
KubínM.1989; Establishment of 2-docosanol as a cellular marker compound in the identification of Mycobacterium xenopi . J Clin Microbiol 27:2388–2390
LuquinM,
AusinaV,
López CalahorraF,
BeldaF,
García BarcelóM,
CelmaC,
PratsG.1991; Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria. J Clin Microbiol 29:120–130
MüllerK.-D,
SchmidE. N,
KroppenstedtR. M.1998; Improved identification of mycobacteria by using the Microbial Identification System in combination with additional trimethylsulfonium hydroxide pyrolysis. J Clin Microbiol 36:2477–2480
PortaelsF,
DawsonD. J,
LarssonL,
RigoutsL.1993; Biochemical properties and fatty acid composition of Mycobacterium haemophilum : study of 16 isolates from Australian patients. J Clin Microbiol 31:26–30
SpringerB,
WuW.-K,
BodmerT.10 other authors1996; Isolation and characterization of a unique group of slowly growing mycobacteria: description of Mycobacterium lentiflavum sp. nov. J Clin Microbiol 34:1100–1107
TorkkoP,
SuutariM,
SuomalainenS,
PaulinL,
LarssonL,
KatilaM.-L.1998; Separation among species of Mycobacterium terrae complex by lipid analyses: comparison with biochemical tests and 16S rRNA sequencing. J Clin Microbiol 36:499–505
TorkkoP,
SuomalainenS,
IivanainenE,
SuutariM,
TortoliE,
PaulinL,
KatilaM.-L.2000; Mycobacterium xenopi and related organisms isolated from stream waters in Finland and description of Mycobacterium botniense sp. nov. Int J Syst Evol Microbiol 50:283–289[CrossRef]
TorkkoP,
SuomalainenS,
IivanainenE.7 other authors2001; Characterization of Mycobacterium bohemicum isolated from human, veterinary, and environmental sources. J Clin Microbiol 39:207–211[CrossRef]
TortoliE,
NanettiA,
PiersimoniC.11 other authors2001a; Performance assessment of new multiplex probe assay for identification of mycobacteria. J Clin Microbiol 39:1079–1084[CrossRef]
Vincent Lévy-FrébaultV,
PortaelsF.1992; Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species. Int J Syst Bacteriol 42:315–323[CrossRef]
WayneL. G,
GoodR. C,
TsangA.13 other authors1993; Serovar determination and molecular taxonomic correlation in Mycobacterium avium , Mycobacterium intracellulare , and Mycobacterium scrofulaceum : a cooperative study of the International Working Group on Mycobacterial Taxonomy. Int J Syst Bacteriol 43:482–489[CrossRef]
WilsonR. W,
SteingrubeV. A,
BöttgerE. C.11 other authors2001; Mycobacterium immunogenum sp. nov., a novel species related to Mycobacterium abscessus and associated with clinical disease, pseudo-outbreaks and contaminated metalworking fluids: an international cooperative study on mycobacterial taxonomy. Int J Syst Evol Microbiol 51:1751–1764[CrossRef]
WolfeJ,
TurenneC,
AlfaM,
HardingG,
ThibertL,
KabaniA.2000; Mycobacterium branderi from both a hand infection and a case of pulmonary disease. J Clin Microbiol 38:3896–3899