1887

Abstract

Leptospirosis is a major public health problem in Andaman Islands. Several strains of have been isolated from the Andamans over the years. Leptospires isolated recently from human cases were compared with one of the earliest available isolates from these islands, dating back to 1929, to study their serological and genetic relatedness. Randomly amplified polymorphic DNA (RAPD) fingerprints of the isolates, generated with a primer used previously to differentiate between species and serovars, revealed that some of the recent isolates were genetically identical to the 1929 isolate. The antigenic properties of these strains, as revealed by microscopic agglutination tests with group-specific rabbit antisera and mAbs, were also similar. These findings suggest that a strain originally isolated in 1929 has possibly persisted in these islands for over 70 years and continues to cause acute leptospirosis in humans.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05101-0
2003-10-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/jmm/52/10/jm521011.html?itemId=/content/journal/jmm/10.1099/jmm.0.05101-0&mimeType=html&fmt=ahah

References

  1. Boom, R., Sol, C. J. A., Salimans, M. M. M., Jansen, C. L., Wertheim-van Dillen, P. M. E. & van der Noordaa, J. ( 1990;). Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28, 495–503.
    [Google Scholar]
  2. Brown, P. D. & Levett, P. N. ( 1997;). Differentiation of Leptospira species and serovars by PCR-restriction endonuclease analysis, arbitrarily primed PCR and low-stringency PCR. J Med Microbiol 46, 173–181.[CrossRef]
    [Google Scholar]
  3. Dikken, H. & Kmety, E. ( 1978;). Serological typing methods of leptospires. Methods Microbiol 11, 259–307.
    [Google Scholar]
  4. Hardham, J. M. & Rosey, E. L. ( 2000;). Antibiotic selective markers and spirochete genetics. J Mol Microbiol Biotechnol 2, 425–432.
    [Google Scholar]
  5. Levett, P. N. ( 2001;). Leptospirosis. Clin Microbiol Rev 14, 296–326.[CrossRef]
    [Google Scholar]
  6. Plank, R. & Dean, D. ( 2000;). Overview of the epidemiology, microbiology, and pathogenesis of Leptospira spp.in humans. Microbes Infect 2, 1265–1276.[CrossRef]
    [Google Scholar]
  7. Ramdass, P., Latha, D., Senthilkumar, A., Srinivasan, P. & Saranya, N. ( 2002;). Arbitrarily primed PCR – a rapid and simple method for typing of leptospiral serovars. Indian J Med Microbiol 20, 25–28.
    [Google Scholar]
  8. Sehgal, S. C., Murhekar, M. V. & Sugunan, A. P. ( 1995;). Outbreak of leptospirosis with pulmonary involvement in North Andaman. Indian J Med Res 102, 9–12.
    [Google Scholar]
  9. Sehgal, S. C., Vijayachari, P., Smythe, L. D. & 7 other authors ( 2000;). Lai-like Leptospira from the Andaman Islands. Indian J Med Res 112, 135–139.
    [Google Scholar]
  10. Taylor, J. & Goyle, A. N. ( 1931;). Leptospirosis in the Andamans. Supplement to Indian Journal of Medical Research, Memoir no. 20. Indian Research Fund Association. Calcutta: Thacker, Spink & Co.
  11. Taylor, K. A., Barbour, A. G. & Thomas, D. D. ( 1991;). Pulsed-field gel electrophoretic analysis of leptospiral DNA. Infect Immun 59, 323–329.
    [Google Scholar]
  12. Wolff, J. W. ( 1954;). In The Laboratory Diagnosis of Leptospirosis, pp. 39–62. Springfield, IL: Charles C. Thomas.
  13. Yang, C. W., Wu, M. S. & Pan, M. J. ( 2001;). Leptospirosis renal disease. Nephrol Dial Transplant 16 (Suppl. 5), 73–77.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05101-0
Loading
/content/journal/jmm/10.1099/jmm.0.05101-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error