1887

Abstract

This study reports on the emergence of -harbouring coagulase-negative staphylococci (CoNS) among patients who received linezolid therapy in two hospitals in Hangzhou, China. The mechanisms of resistance and transmission were analysed for these resistant isolates. Eight isolates, one isolate and one isolate, obtained from patients who had received linezolid therapy in two hospitals in Hangzhou, China, were confirmed as linezolid resistant, with MICs ranging from 8 to >256 mg l. The linezolid usage data of the ten patients before isolation of the linezolid-resistant CoNS were collected. PFGE analysis showed that the eight isolates from the two hospitals belonged to the same clone. Nine of the linezolid-resistant CoNS isolates carried the gene, which was located on plasmids of a similar size. A 5.3 kb fragment containing the gene, revealing 99 % identity to the sequence of the -harbouring plasmid pSS-01 reported previously, was determined by PCR mapping for all -positive isolates, and the gene was flanked by two copies of IS-like elements. Thus, these results document the emergence of linezolid-resistant CoNS isolates carrying the gene in Hangzhou, China. Effective nosocomial infection control strategies and the judicious use of antibiotics will be required to prevent further spread of this resistance mechanism.

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2013-06-01
2019-10-21
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References

  1. Bannerman T. L., Hancock G. A., Tenover F. C., Miller J. M.. ( 1995;). Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus. . J Clin Microbiol 33:, 551–555.[PubMed]
    [Google Scholar]
  2. Bonilla H., Huband M. D., Seidel J., Schmidt H., Lescoe M., McCurdy S. P., Lemmon M. M., Brennan L. A., Tait-Kamradt A.. & other authors ( 2010;). Multicity outbreak of linezolid-resistant Staphylococcus epidermidis associated with clonal spread of a cfr-containing strain. . Clin Infect Dis 51:, 796–800. [CrossRef][PubMed]
    [Google Scholar]
  3. CLSI (2012). Performance Standards for Antimicrobial Susceptibility Testing, 22nd Informational Supplement. M100-S22. Wayne, PA: Clinical and Laboratory Standards Institute.
  4. Kehrenberg C., Aarestrup F. M., Schwarz S.. ( 2007;). IS21-558 insertion sequences are involved in the mobility of the multiresistance gene cfr. . Antimicrob Agents Chemother 51:, 483–487. [CrossRef][PubMed]
    [Google Scholar]
  5. Long K. S., Poehlsgaard J., Kehrenberg C., Schwarz S., Vester B.. ( 2006;). The Cfr rRNA methyltransferase confers resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A antibiotics. . Antimicrob Agents Chemother 50:, 2500–2505. [CrossRef][PubMed]
    [Google Scholar]
  6. Marshall S. H., Donskey C. J., Hutton-Thomas R., Salata R. A., Rice L. B.. ( 2002;). Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis. . Antimicrob Agents Chemother 46:, 3334–3336. [CrossRef][PubMed]
    [Google Scholar]
  7. Meka V. G., Gold H. S.. ( 2004;). Antimicrobial resistance to linezolid. . Clin Infect Dis 39:, 1010–1015. [CrossRef][PubMed]
    [Google Scholar]
  8. Morales G., Picazo J. J., Baos E., Candel F. J., Arribi A., Peláez B., Andrade R., de la Torre M. A., Fereres J., Sánchez-García M.. ( 2010;). Resistance to linezolid is mediated by the cfr gene in the first report of an outbreak of linezolid-resistant Staphylococcus aureus. . Clin Infect Dis 50:, 821–825. [CrossRef][PubMed]
    [Google Scholar]
  9. Piette A., Verschraegen G.. ( 2009;). Role of coagulase-negative staphylococci in human disease. . Vet Microbiol 134:, 45–54. [CrossRef][PubMed]
    [Google Scholar]
  10. Toh S. M., Xiong L., Arias C. A., Villegas M. V., Lolans K., Quinn J., Mankin A. S.. ( 2007;). Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureus resistant to the synthetic antibiotic linezolid. . Mol Microbiol 64:, 1506–1514. [CrossRef][PubMed]
    [Google Scholar]
  11. Wang Y., Zhang W., Wang J., Wu C., Shen Z., Fu X., Yan Y., Zhang Q., Schwarz S., Shen J.. ( 2012;). Distribution of the multidrug resistance gene cfr in Staphylococcus species isolates from swine farms in China. . Antimicrob Agents Chemother 56:, 1485–1490. [CrossRef][PubMed]
    [Google Scholar]
  12. Widerström M., Monsen T., Karlsson C., Wiström J.. ( 2006;). Molecular epidemiology of meticillin-resistant coagulase-negative staphylococci in a Swedish county hospital: evidence of intra- and interhospital clonal spread. . J Hosp Infect 64:, 177–183. [CrossRef][PubMed]
    [Google Scholar]
  13. Wilson P., Andrews J. A., Charlesworth R., Walesby R., Singer M., Farrell D. J., Robbins M.. ( 2003;). Linezolid resistance in clinical isolates of Staphylococcus aureus. . J Antimicrob Chemother 51:, 186–188. [CrossRef][PubMed]
    [Google Scholar]
  14. Witte W., Cuny C.. ( 2011;). Emergence and spread of cfr-mediated multiresistance in staphylococci: an interdisciplinary challenge. . Future Microbiol 6:, 925–931. [CrossRef][PubMed]
    [Google Scholar]
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