RT Journal Article SR Electronic(1) A1 Khan, Z.U. A1 Al-Anezi, A.A. A1 Chandy, R. A1 Xu, J.YR 2003 T1 Disseminated cryptococcosis in an AIDS patient caused by a canavanine-resistant strain of Cryptococcus neoformans var. grubii JF Journal of Medical Microbiology, VO 52 IS 3 SP 271 OP 275 DO https://doi.org/10.1099/jmm.0.05097-0 PB Microbiology Society, SN 1473-5644, AB A case of disseminated cryptococcosis caused by Cryptococcus neoformans var. grubii is presented in a male diabetic who had AIDS. The diagnosis was based upon the isolation and identification of the aetiological agent from a lymph-node biopsy, cerebrospinal fluid and sputum. The isolate formed spherical, encapsulated yeast cells, produced cherry-brown colonies on niger-seed agar, grew on canavanine-glycine-bromothymol blue (CGB) medium, changing its colour from greenish yellow to blue, and hydrolysed urea weakly in the presence of 100 μM EDTA. The strain was unable to assimilate d-proline and, serologically, it was untypable. The identity of the isolate as C. neoformans var. grubii, serotype A, possessing a mating-type allele Aα, was confirmed by crossing with standard laboratory test strains and by performing PCR with the mating-type α allele-specific primer of the STE12 gene and with serotype (A and D)- and mating type (a and α)-specific primers of the STE20 gene. To the best of our knowledge, this is the first report of disseminated cryptococcosis in an AIDS patient caused by a canavanine-resistant strain of C. neoformans var. grubii, serotype A, possessing mating type allele Aα; the strain is probably a hybrid. The report suggests that, in the absence of a clear-cut serotyping result, a positive CGB reaction alone is not sufficient for intervarietal discrimination and additional confirmatory evidence is required., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.05097-0