The performances of recombinant borrelial BBK32 proteins as antigens in the serology of erythema migrans (EM) were evaluated in an ELISA. Serum samples were obtained from 75 patients from different geographic areas where three borrelial species, Borrelia burgdorferi sensu stricto, Borrelia afzelii or Borrelia garinii, cause Lyme borreliosis. Antibodies to variant BBK32 proteins were compared with anti-flagella or with anti-IR6 peptide antibodies. In IgG ELISA at presentation of EM, 65/75 (87 %) patients had antibodies to one or more variants of BBK32, 29/75 (39 %) had antibodies to flagella and 29/75 (39 %) had antibodies to the VlsE IR6 peptide antigen. The immunoreactivity against variant BBK32 proteins differed in patients from different geographic regions. The present results suggest that the BBK32 proteins used in combination or in parallel may improve the laboratory diagnosis of EM.
Aguero-RosenfeldM. E,
NowakowskiJ,
BittkerS,
CooperD,
NadelmanR. B,
WormserG. P.1996; Evolution of the serologic response to Borrelia burgdorferi in treated patients with culture-confirmed erythema migrans. J Clin Microbiol 34:1–9
HeikkiläT.,
SeppäläI.,
SaxenH.,
PaneliusJ.,
PeltomaaM.,
JulinT.,
CarlssonS.-A.,
LahdenneP.2002; Recombinant BBK32 protein in serodiagnosis of early and late Lyme borreliosis. J Clin Microbiol 40:1174–1180[CrossRef]
HuppertzH.-I,
BöhmeM,
StandaertS. M,
KarchH,
PlotkinS. A.1999; Incidence of Lyme borreliosis in the Wurzburg region of Germany. Eur J Clin Microbiol Infect Dis 18:697–703[CrossRef]
JunttilaJ,
PeltomaaM,
SoiniH,
MarjamäkiM,
ViljanenM. K.1999; Prevalence of Borrelia burgdorferi in Ixodes ricinus ticks in urban recreational areas of Helsinki. J Clin Microbiol 37:1361–1365
LiangF. T,
SteereA. C,
MarquesA. R,
JohnsonB. J. B,
MillerJ. N,
PhilippM. T.1999; Sensitive and specific serodiagnosis of Lyme disease by enzyme-linked immunosorbent assay with a peptide based on an immunodominant conserved region of Borrelia burgdorferi VlsE. J Clin Microbiol 37:3990–3996
MitchellP. D,
ReedK. D,
AspesletT. L,
VandermauseM. F,
MeslkiJ. W.1994; Comparison of four immunoserologic assays for detection of antibodies to Borrelia burgdorferi in patients with culture-positive erythema migrans. J Clin Microbiol 32:1958–1962
NowakowskiJ,
SchwartzI,
LiverisD.7 other authors2001; Laboratory diagnostic techniques for patients with early erythema migrans: a comparison of different techniques. Clin Infect Dis 33:2023–2027[CrossRef]
OksiJ,
MarttilaH,
SoiniH,
AhoH,
UksilaJ,
ViljanenM. K.2001; Early dissemination of Borrelia burgdorferi without generalized symptoms in patients with erythema migrans. APMIS 109:581–588[CrossRef]
SeppäläI. J. T,
KroneldR,
SchaumanK,
ForsenK. O,
LasseniusR.1994; Diagnosis of Lyme borreliosis: non-specific serological reactions with Borrelia burgdorferi sonicate antigen caused by IgG2 antibodies. J Med Microbiol 40:293–302[CrossRef]
VazA,
GlicksteinL,
FieldJ. A,
McHughG,
SikandV. K,
DamleN,
SteereA. C.2001; Cellular and humoral immune responses to Borrelia burgdorferi antigens in patients with culture-positive early Lyme disease. Infect Immun 69:7437–7444[CrossRef]