1887

Abstract

Rapid, sensitive and low-cost methods are needed urgently for the detection of in clinical samples, especially in developing countries. To this end, the clinical performance of TB (a bacteriophage-based method) has been studied in parallel with microscopy, standard microbiological culture and in-house ISbased PCR methods. A total of 64 samples, including 42 sputum samples and 22 urine samples, were tested in this study. The sensitivity, specificity and overall accuracy values for the TB assay relative to that of culture were respectively 76.5, 95 and 90 %. The corresponding values for the in-house ISbased PCR assay were 88, 91 and 90 % and, for ZiehlNeelsen staining, were 59, 95 and 85 %. TB gave better clinical performance with urine samples than with sputum samples (sensitivity, specificity and overall accuracy were 100 % with urine samples and 64, 93 and 84 % with sputum samples). The 100 % sensitivity of TB was higher than that of the corresponding values for PCR (67 %) with urine samples. In conclusion, TB proved to be sensitive, cheap relative to the PCR and rapid. It is able to detect in clinical samples within 1 day, reducing the time to diagnosis in comparison with culture.

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2003-04-01
2019-11-12
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