Inability of outer-surface protein C (OspC)-primed mice to elicit a protective anamnestic immune response to a tick-transmitted challenge of Free

Abstract

A one-inoculation regimen of recombinant outer-surface protein C (OspC), which has been demonstrated to elicit protective immunity against a tick-borne challenge of , was administered to outbred mice. Following seroconversion, the serum antibody titre against OspC was allowed to wane with time until there was little or no detection of anti-OspC antibodies by immunoblot. The mice were then challenged with an infectious dose of by tick transmission. Eleven of 12 OspC-primed mice subsequently became infected by , demonstrating that a protective anamnestic response was not generated in these mice following the introduction of infectious OspC-expressing spirochaetes.

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2003-07-01
2024-03-28
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References

  1. Cox D. L, Akins D. R, Bourell K. W, Lahdenne P, Norgard M. V, Radolf J. D. 1996; Limited surface exposure of Borrelia burgdorferi outer surface lipoproteins. Proc Natl Acad Sci U S A 93:7973–7978 [CrossRef]
    [Google Scholar]
  2. de Silva A. M, Telford S. R III, Brunet L. R, Barthold S. W, Fikrig E. 1996; Borrelia burgdorferi OspA is an arthropod-specific transmission-blocking Lyme disease vaccine. J Exp Med 183:271–275 [CrossRef]
    [Google Scholar]
  3. Dolan M. C, Maupin G. O, Panella N. A, Golde W. T, Piesman J. 1997; Vector competence of Ixodes scapularis , I.spinipalpis , and Dermacentor andersoni (Acari: Ixodidae) in transmitting Borrelia burgdorferi , the etiologic agent of Lyme disease. J Med Entomol 34:128–135 [CrossRef]
    [Google Scholar]
  4. Dressler F, Whalen J. A, Reinhardt B. N, Steere A. C. 1993; Western blotting in the serodiagnosis of Lyme disease. J Infect Dis 167:392–400 [CrossRef]
    [Google Scholar]
  5. Exner M. M, Wu X, Blanco D. R, Miller J. N, Lovett M. A. 2000; Protection elicited by native outer membrane protein Oms66 (p66) against host-adapted Borrelia burgdorferi : conformational nature of bactericidal epitopes. Infect Immun 68:2647–2654 [CrossRef]
    [Google Scholar]
  6. Fikrig E, Barthold S. W, Kantor F. S, Flavell R. A. 1990; Protection of mice against the Lyme disease agent by immunizing with recombinant OspA. Science 250:553–556 [CrossRef]
    [Google Scholar]
  7. Fikrig E, Feng W, Barthold S. W, Telford S. R. III, Flavell R. A. 2000; Arthropod- and host-specific Borrelia burgdorferi bbk32 expression and the inhibition of spirochete transmission. J Immunol 164:5344–5351 [CrossRef]
    [Google Scholar]
  8. Gilmore R. D Jr, Mbow M. L. 1999; Conformational nature of the Borrelia burgdorferi B31 outer surface protein C protective epitope. Infect Immun 67:5463–5469
    [Google Scholar]
  9. Gilmore R. D Jr, Piesman J. 2000; Inhibition of Borrelia burgdorferi migration from the midgut to the salivary glands following feeding by ticks on OspC-immunized mice. Infect Immun 68:411–414 [CrossRef]
    [Google Scholar]
  10. Gilmore R. D Jr, Kappel K. J, Dolan M. C, Burkot T. R, Johnson B. J. 1996; Outer surface protein C (OspC), but not P39, is a protective immunogen against a tick-transmitted Borrelia burgdorferi challenge: evidence for a conformational protective epitope in OspC. Infect Immun 64:2234–2239
    [Google Scholar]
  11. Hagman K. E, Lahdenne P, Popova T. G, Porcella S. F, Akins D. R, Radolf J. D, Norgard M. V. 1998; Decorin-binding protein of Borrelia burgdorferi is encoded within a two-gene operon and is protective in the murine model of Lyme borreliosis. Infect Immun 66:2674–2683
    [Google Scholar]
  12. Hanson M. S, Cassatt D. R, Guo B. P, Patel N. K, McCarthy M. P, Dorward D. W, Hook M. 1998; Active and passive immunity against Borrelia burgdorferi decorin binding protein A (DbpA) protects against infection. Infect Immun 66:2143–2153
    [Google Scholar]
  13. Leuba-Garcia S, Martinez R, Gern L. 1998; Expression of outer surface proteins A and C of Borrelia afzelii in Ixodes ricinis ticks and in the skin of mice. Zentbl Bakteriol 287:475–484 [CrossRef]
    [Google Scholar]
  14. Liang F. T, Jacobs M. B, Bowers L. C, Philipp M. T. 2002; An immune evasion mechanism for spirochetal persistence in Lyme borreliosis. J Exp Med 195:415–422 [CrossRef]
    [Google Scholar]
  15. Mbow M. L, Gilmore R. D Jr, Titus R. G. 1999; An OspC-specific monoclonal antibody passively protects mice from tick-transmitted infection by Borrelia burgdorferi B31. Infect Immun 67:5470–5472
    [Google Scholar]
  16. Ohnishi J, Piesman J, de Silva A. M. 2001; Antigenic and genetic heterogeneity of Borrelia burgdorferi populations transmitted by ticks. Proc Natl Acad Sci U S A 98:670–675 [CrossRef]
    [Google Scholar]
  17. Padula S. J, Dias F, Sampieri A, Craven R. B, Ryan R. W. 1994; Use of recombinant OspC from Borrelia burgdorferi for serodiagnosis of early Lyme disease. J Clin Microbiol 32:1733–1738
    [Google Scholar]
  18. Preac-Mursic V, Wilske B, Patsouris E. 7 other authors 1992; Active immunization with pC protein of Borrelia burgdorferi protects gerbils against B.burgdorferi infection. Infection 20:342–349 [CrossRef]
    [Google Scholar]
  19. Probert W. S, Crawford M, LeFebvre R. B. 1997; Antibodies to OspB prevent infection of C3H mice challenged with Borrelia burgdorferi isolates expressing truncated OspB antigens. Vaccine 15:15–19 [CrossRef]
    [Google Scholar]
  20. Schaible U. E, Kramer M. D, Eichmann K, Modolell M, Museteanu C, Simon M. M. 1990; Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Proc Natl Acad Sci U S A 87:3768–3772 [CrossRef]
    [Google Scholar]
  21. Schwan T. G, Piesman J. 2000; Temporal changes in outer surface proteins A and C of the Lyme disease-associated spirochete, Borrelia burgdorferi , during the chain of infection in ticks and mice. J Clin Microbiol 38:382–388
    [Google Scholar]
  22. Schwan T. G, Piesman J, Golde W. T, Dolan M. C, Rosa P. A. 1995; Induction of an outer surface protein on Borrelia burgdorferi during tick feeding. Proc Natl Acad Sci U S A 92:2909–2913 [CrossRef]
    [Google Scholar]
  23. Sigal L. H, Zahradnik J. M, Lavin P. 16 other authors 1998; A vaccine consisting of recombinant Borrelia burgdorferi outer-surface protein A to prevent Lyme disease.Recombinant Outer-Surface Protein A Lyme Disease Vaccine Study Consortium. N Engl J Med 339:216–222 [CrossRef]
    [Google Scholar]
  24. Simon M. M, Schaible U. E, Kramer M. D, Eckerskorn C, Museteanu C, Muller-Hermelink H. K, Wallich R. 1991; Recombinant outer surface protein A from Borrelia burgdorferi induces antibodies protective against spirochetal infection in mice. J Infect Dis 164:123–132 [CrossRef]
    [Google Scholar]
  25. Sinsky R. J, Piesman J. 1989; Ear punch biopsy method for detection and isolation of Borrelia burgdorferi from rodents. J Clin Microbiol 27:1723–1727
    [Google Scholar]
  26. Steere A. C, Sikand V. K, Meurice F. 8 other authors 1998; Vaccination against Lyme disease with recombinant Borrelia burgdorferi outer-surface lipoprotein A with adjuvant.Lyme Disease Vaccine Study Group. N Engl J Med 339:209–215 [CrossRef]
    [Google Scholar]
  27. Zhong W, Gern L, Stehle T, Museteanu C, Kramer M, Wallich R, Simon M. M. 1999; Resolution of experimental and tick-borne Borrelia burgdorferi infection in mice by passive, but not active immunization using recombinant OspC. Eur J Immunol 29:946–957 [CrossRef]
    [Google Scholar]
  28. Zhong W, Stehle T, Museteanu C, Siebers A, Gern L, Kramer M, Wallich R, Simon M. M. 1997; Therapeutic passive vaccination against chronic Lyme disease in mice. Proc Natl Acad Sci U S A 94:12533–12538 [CrossRef]
    [Google Scholar]
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