1887

Abstract

A one-inoculation regimen of recombinant outer-surface protein C (OspC), which has been demonstrated to elicit protective immunity against a tick-borne challenge of , was administered to outbred mice. Following seroconversion, the serum antibody titre against OspC was allowed to wane with time until there was little or no detection of anti-OspC antibodies by immunoblot. The mice were then challenged with an infectious dose of by tick transmission. Eleven of 12 OspC-primed mice subsequently became infected by , demonstrating that a protective anamnestic response was not generated in these mice following the introduction of infectious OspC-expressing spirochaetes.

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2003-07-01
2019-11-23
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vol. , part 7, pp. 551 - 556

Confocal microscopy of in the salivary glands of a tick that was removed at 72 h of feeding on the mouse that was protected from infection. was double-stained as described in the text. (a) The left panel shows (stained green) within the salivary glands under a fluorescein filter. The right panel shows the same field under a Texas red filter. that expresses OspC would be observed as red and is not apparent. (b) A different field from the salivary glands shown in (a), indicating the presence of an OspC-expressing cell. The left panel is viewed with the fluorescein filter. The right panel shows the same field with the Texas red filter.



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