1887

Journal of Medical Microbiology

Volume 52, Issue 4

Expression of heterologous O-antigen in KIM does not affect virulence by the intravenous route Free

Abstract

All strains of examined have been found to lack an O-antigen. In other members of the , the rough phenotype often results in attenuation. However, is the aetiological agent of bubonic plague. In evolving from the ancestral enteropathogenic , and with the development of an arthropod-vectored systemic pathogenesis, smooth LPS production is not necessary for virulence and the metabolic burden has been alleviated by inactivation of the O-antigen biosynthetic operon. To investigate this, strain KIM D27 was transformed with a plasmid carrying the operon encoding the O-antigen of O : 3. Expression of the O-antigen could be detected in silver-stained gels. The receptor for bacteriophage ϕYeO3-12 has been shown to be O-antigen, and infection by this bacteriophage results in lysis of O : 3. Expression of the O-antigen in conferred sensitivity to lysis by ϕYeO3-12. The O-antigen-expressing clone was shown to be as virulent in mice by the intravenous route of challenge as the rough wild-type. Assays showed no alteration in the ability of to resist lysis by cationic antimicrobial peptides, serum or polymyxin.

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© 2003 Society for General Microbiology
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2003-04-01
2019-10-21
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Expression of heterologous O-antigen in Yersinia pestis KIM does not affect virulence by the intravenous route
J. Med. Microbiol. 52, 289 (2003); https://doi.org/10.1099/jmm.0.05044-0
/content/journal/jmm/10.1099/jmm.0.05044-0
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