1887

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Volume 52, Issue 4

Experimental infection of layer hens with a human isolate of Free

Abstract

The anaerobic intestinal spirochaete commonly colonizes the large intestine of a number of species, including chickens and human beings. The purpose of the current study was to determine whether an isolate of recovered from an HIV-infected patient with diarrhoea could infect and cause disease in adult chickens. Over a 4-week period following experimental infection, a group of eight inoculated chickens showed a persistent and significant increase in faecal water content (∼6–7 %). The faeces of three of the eight birds became culture-positive, and remained so. At post-mortem examination, no specific pathological changes were found, and no spirochaetal attachment to the caecal epithelium was observed. These findings confirm that strains can infect across species barriers and cause chronic mild diarrhoea in intact adult chickens.

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© 2003 Society for General Microbiology
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2003-04-01
2024-04-25
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References

  1. Antonakopoulos G, Newman J, Wilkinson M. 1982; Intestinal spirochaetosis: an electron microscopic study of an unusual case. Histopathology 6:477–478 [CrossRef]
     [Google Scholar]
  2. Atyeo R. F, Oxberry S. L, Combs B. G, Hampson D. J. 1998; Development and evaluation of polymerase chain reaction tests as an aid to diagnosis of swine dysentery and intestinal spirochaetosis. Lett Appl Microbiol 26:126–130 [CrossRef]
     [Google Scholar]
  3. Barrett S. P. 1990; Intestinal spirochaetes in a Gulf Arab population. Epidemiol Infect 104:261–266 [CrossRef]
     [Google Scholar]
  4. Brooke C. J, Clair A. N, Mikosza A. S. J, Riley T. V, Hampson D. J. 2001; Carriage of intestinal spirochaetes by humans: epidemiological data from Western Australia. Epidemiol Infect 127:369–374
     [Google Scholar]
  5. Douglas J. G, Crucioli V. 1981; Spirochaetosis: a remediable cause of diarrhoea and rectal bleeding?. BMJ 283:1362 [CrossRef]
     [Google Scholar]
  6. Duhamel G. E, Trott D. J, Muniappa N, Mathiesen M. R, Tarasiuk K, Lee J. I, Hampson D. J. 1998; Canine intestinal spirochetes consist of Serpulina pilosicoli and a newly identified group provisionally designated ‘‘ Serpulina canis ’’. sp nov. J Clin Microbiol 36:2264–2270
     [Google Scholar]
  7. Dwars R. M, Davelaar F. G, Smit H. F. 1992; Infection of broiler chicks ( Gallus domesticus ) with human intestinal spirochaetes. Avian Pathol 21:559–568 [CrossRef]
     [Google Scholar]
  8. Fournié-Amazouz E, Baranton G, Carlier J. P. 7 other authors 1995; Isolations of intestinal spirochaetes from the blood of human patients. J Hosp Infect 30:160–162 [CrossRef]
     [Google Scholar]
  9. Gad A, Willén R, Furugård K, Fors B, Hradsky M. 1977; Intestinal spirochaetosis as a cause of longstanding diarrhoea. Uppsala J Med Sci 82:49–54 [CrossRef]
     [Google Scholar]
  10. Guccion J. G, Benator D. A, Zeller J, Termanini B, Saini N. 1995; Intestinal spirochetosis and acquired immunodeficiency syndrome: ultrastructural studies of two cases. Ultrastruct Pathol 19:15–22 [CrossRef]
     [Google Scholar]
  11. Heine R. G, Ward P. B, Mikosza A. S. J, Bennett-Wood V, Robins-Browne R. M, Hampson D. J. 2001; Brachyspira aalborgi infection in four Australian children. J Gastroenterol Hepatol 16:872–875 [CrossRef]
     [Google Scholar]
  12. Hopwood D. E, Pethick D. W, Hampson D. J. 2002; Increasing the viscosity of the intestinal contents stimulates proliferation of enterotoxigenic Escherichia coli and Brachyspira pilosicoli in weaner pigs. Br J Nutr 88:523–532 [CrossRef]
     [Google Scholar]
  13. Jamshidi A, Hampson D. J. 2002; Zinc bacitracin enhances colonization by the intestinal spirochaete Brachyspira pilosicoli in experimentally infected layer hens. Avian Pathol 31:293–298 [CrossRef]
     [Google Scholar]
  14. Jenkinson S. R, Wingar C. R. 1981; Selective medium for the isolation of Treponema hyodysenteriae . Vet Rec 109:384–385 [CrossRef]
     [Google Scholar]
  15. Kanavaki S, Mantadakis E, Thomakos N, Pefanis A, Matsiota-Bernard P, Karabela S, Samonis G. 2002; Brachyspira Serpulina) pilosicoli spirochetemia in an immunocompromised patient. Infection 30:175–177 [CrossRef]
     [Google Scholar]
  16. Käsbohrer A, Gelderblom H. R, Arasteh K, Heise W, Grosse G, L'age M, Schonberg A, Koch M. A, Pauli G. 1990; Intestinale spirochätose bei HIV-infektion. Dtsch Med Wochenschr 115:1499–1506 [CrossRef]
     [Google Scholar]
  17. Koopman M. B. H, Käsbohrer A, Beckmann G, van der Zeijst B. A. M, Kusters J. G. 1993; Genetic similarity of intestinal spirochetes from humans and various animal species. J Clin Microbiol 31:711–716
     [Google Scholar]
  18. Kostman J. R, Patel M, Catalano E, Camacho J, Hoffpauir J, DiNubile M. J. 1995; Invasive colitis and hepatitis due to previously uncharacterized spirochetes in patients with advanced human immunodeficiency virus infection. Clin Infect Dis 21:1159–1165 [CrossRef]
     [Google Scholar]
  19. Kunkle R. A, Harris D. L, Kinyon J. M. 1986; Autoclaved liquid medium for propagation of Treponema hyodysenteriae . J Clin Microbiol 24:669–671
     [Google Scholar]
  20. Lambert T, Goursot G. 1982; Diarrhée aiguë avec hémocultures et coprocultures positives à Tréponema . Med Malad Infect 12:276–278 [CrossRef]
     [Google Scholar]
  21. Lee J. I, Hampson D. J. 1992; Intestinal spirochaetes colonizing aborigines from communities in the remote north of Western Australia. Epidemiol Infect 109:133–141
     [Google Scholar]
  22. Mikosza A. S. J, La T, Brooke C. J, Lindboe C. F, Ward P. B, Heine R. G, Guccion J. G, de Boer W. B, Hampson D. J. 1999; PCR amplification from fixed tissue indicates frequent involvement of Brachyspira aalborgi in human intestinal spirochetosis. J Clin Microbiol 37:2093–2098
     [Google Scholar]
  23. Mikosza A. S. J, La T, Margawani K. R, Brooke C. J, Hampson D. J. 2001; PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces. FEMS Microbiol Lett 197:167–170 [CrossRef]
     [Google Scholar]
  24. Muniappa N, Duhamel G. E, Mathiesen M. R, Bargar T. W. 1996; Light microscopic and ultrastructural changes in the ceca of chicks inoculated with human and canine Serpulina pilosicoli . Vet Pathol 33:542–550 [CrossRef]
     [Google Scholar]
  25. Oxberry S. L, Trott D. J, Hampson D. J. 1998; Serpulina pilosicoli , waterbirds and water: potential sources of infection for humans and other animals. Epidemiol Infect 121:219–225 [CrossRef]
     [Google Scholar]
  26. Padmanabhan V, Dahlstrom J, Maxwell L, Kaye G, Clarke A, Barratt P. J. 1996; Invasive intestinal spirochetosis: a report of three cases. Pathology 28:283–286 [CrossRef]
     [Google Scholar]
  27. Rayment S. J, Barrett S. P, Livesley M. A. 1997; Sub-specific differentiation of intestinal spirochaete isolates by macrorestriction fragment profiling. Microbiology 143:2923–2929 [CrossRef]
     [Google Scholar]
  28. Rodgers F. G, Rodgers C, Shelton A. P, Hawkey C. J. 1986; Proposed pathogenic mechanism for the diarrhea associated with human intestinal spirochetes. Am J Clin Pathol 86:679–682
     [Google Scholar]
  29. Sacco R. E, Trampel D. W, Wannemuehler M. J. 1997; Experimental infection of C3H mice with avian, porcine, or human isolates of Serpulina pilosicoli . Infect Immun 65:5349–5353
     [Google Scholar]
  30. Stephens C. P, Hampson D. J. 1999; Prevalence and disease association of intestinal spirochaetes in chickens in eastern Australia. Avian Pathol 28:447–454 [CrossRef]
     [Google Scholar]
  31. Stephens C. P, Hampson D. J. 2001; Intestinal spirochaete infections of chickens: a review of disease associations, epidemiology and control. Anim Health Res Rev 2:83–91
     [Google Scholar]
  32. Stephens C. P, Hampson D. J. 2002a; Experimental infection of broiler breeder hens with the intestinal spirochaete Brachyspira ( Serpulina ) pilosicoli causes reduced egg production. Avian Pathol 31:169–175 [CrossRef]
     [Google Scholar]
  33. Stephens C. P, Hampson D. J. 2002b; Evaluation of tiamulin and lincomycin for the treatment of broiler breeders experimentally infected with the intestinal spirochaete Brachyspira pilosicoli . Avian Pathol 31:299–304 [CrossRef]
     [Google Scholar]
  34. Thomson J. R, Smith W. J, Murray B. P, McOrist S. 1997; Pathogenicity of three strains of Serpulina pilosicoli in pigs with a naturally acquired intestinal flora. Infect Immun 65:3693–3700
     [Google Scholar]
  35. Tompkins D. S, Foulkes S. J, Godwin P. G. R, West A. P. 1986; Isolation and characterisation of intestinal spirochaetes. J Clin Pathol 39:535–541 [CrossRef]
     [Google Scholar]
  36. Trivett-Moore N. L, Gilbert G. L, Law C. L. H, Trott D. J, Hampson D. J. 1998; Isolation of Serpulina pilosicoli from rectal biopsy specimens showing evidence of intestinal spirochetosis. J Clin Microbiol 36:261–265
     [Google Scholar]
  37. Trott D. J, McLaren A. J, Hampson D. J. 1995; Pathogenicity of human and porcine intestinal spirochaetes in one-day-old specific-pathogen-free chicks: an animal model of intestinal spirochetosis. Infect Immun 63:3705–3710
     [Google Scholar]
  38. Trott D. J, Huxtable C. R, Hampson D. J. 1996a; Infection of newly weaned pigs with human and porcine strains of Serpulina pilosicoli. Infect Immun 64:4648–4654
     [Google Scholar]
  39. Trott D. J, Stanton T. B, Jensen N. S, Duhamel G. E, Johnson J. L, Hampson D. J. 1996b; Serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. Int J Syst Bacteriol 46:206–215 [CrossRef]
     [Google Scholar]
  40. Trott D. J, Combs B. G, Mikosza A. S. J. & 7 other authors (1997a). The prevalence of Serpulina pilosicoli in humans and domestic animals in the Eastern Highlands of Papua New Guinea. Epidemiol Infect 119:369–379 [CrossRef]
     [Google Scholar]
  41. Trott D. J, Jensen N. S, Saint Girons I, Oxberry S. L, Stanton T. B, Lindquist D, Hampson D. J. 1997b; Identification and characterization of Serpulina pilosicoli isolates recovered from the blood of critically ill patients. J Clin Microbiol 35:482–485
     [Google Scholar]
  42. Trott D. J, Mikosza A. S. J, Combs B. G, Oxberry S. L, Hampson D. J. 1998; Population genetic analysis of Serpulina pilosicoli and its molecular epidemiology in villages in the Eastern Highlands of Papua New Guinea. Int J Syst Bacteriol 48:659–668 [CrossRef]
     [Google Scholar]
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Experimental infection of layer hens with a human isolate of Brachyspira pilosicoli
J. Med. Microbiol. 52, 361 (2003); https://doi.org/10.1099/jmm.0.05040-0
/content/journal/jmm/10.1099/jmm.0.05040-0
/content/journal/jmm/10.1099/jmm.0.05040-0
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