@article{mbs:/content/journal/jmm/10.1099/jmm.0.05039-0, author = "Low, Yuen L. and Jakubovics, Nicholas S. and Flatman, Jennifer C. and Jenkinson, Howard F. and Smith, Anthony W.", title = "Manganese-dependent regulation of the endocarditis-associated virulence factor EfaA of Enterococcus faecalis", journal= "Journal of Medical Microbiology", year = "2003", volume = "52", number = "2", pages = "113-119", doi = "https://doi.org/10.1099/jmm.0.05039-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.05039-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "There is increasing recognition of the emerging role of manganese regulation and acquisition in some pathogenic bacteria. Expression of the Enterococcus faecalis endocarditis-associated virulence factor EfaA is induced by growth in serum. It is demonstrated here that expression of the efaCBA operon encoding a putative ABC-type transporter is regulated by Mn2+. Transcription of efaCBA and EfaA production were repressed in Mn2+-supplemented medium. A Mn2+-responsive transcriptional regulator, EfaR, sharing 27 % identity with the Corynebacterium diphtheriae diphtheria toxin repressor (DtxR), was identified. In the presence of Mn2+, EfaR protein bound in vitro to the efaC promoter region. Analysis of the E. faecalis V583 genome revealed ten additional putative EfaR-binding sites, suggesting that manganese availability could have a broader regulatory role in infection. The results identify a new Mn2+-sensing regulator in enterococci that regulates the expression of a virulence factor implicated in enterococcal endocarditis.", }