1887

Abstract

Interpretation of the mupirocin E-test for low-level mupirocin-resistant strains has been improved by adding the indicator dye tetrazolium. E-tests were compared with agar dilution methods for assessing mupirocin susceptibility. MICs obtained by the agar dilution method and E-tests showed 89.3 % agreement within 2 log dilution criteria. The agreement between MICs increased to 100 % in the 1 log dilution definition when the indicator dye tetrazolium was added to the E-test. The use of the E-test with tetrazolium reduction is more accurate for determining mupirocin MICs for strains.

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2003-05-01
2020-01-23
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References

  1. Bartlett, R. C. & Mazens, M. F. ( 1979;). Rapid antimicrobial susceptibility test using tetrazolium reduction. Antimicrob Agents Chemother 15, 769–774.[CrossRef]
    [Google Scholar]
  2. Bastos, M. C. F., Mondino, P. J. J., Azevedo, M. L., Santos, K. R. N. & Giambiagi-deMarval, M. ( 1999;). Molecular characterization and transfer among Staphylococcus strains of a plasmid conferring high-level resistance to mupirocin. Eur J Clin Microbiol Infect Dis 18, 393–398.[CrossRef]
    [Google Scholar]
  3. Eltringham, I. ( 1997;). Mupirocin resistance and methicillin-resistant Staphylococcus aureus (MRSA). J Hosp Infect 35, 1–8.
    [Google Scholar]
  4. Hudson, I. R. ( 1994;). The efficacy of intranasal mupirocin in the prevention of staphylococcal infections: a review of recent experience. J Hosp Infect 27, 81–98.[CrossRef]
    [Google Scholar]
  5. NCCLS ( 2001;). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard, 5th edn. NCCLS document M7-A5. Wayne, PA: National Committee for Clinical Laboratory Standards.
  6. Santos, K. R. N., Fonseca, L. S. & Gontijo Filho, P. P. ( 1996;). Emergence of high-level mupirocin resistance in methicillin-resistant Staphylococcus aureus isolated from Brazilian university hospitals. Infect Control Hosp Epidemiol 17, 813–816.[CrossRef]
    [Google Scholar]
  7. Santos, K. R. N., Teixeira, L. M., Leal, G. S., Fonseca, L. S. & Gontijo Filho, P. P. ( 1999;). DNA typing of methicillin-resistant Staphylococcus aureus: isolates and factors associated with nosocomial acquisition in two Brazilian university hospitals. J Med Microbiol 48, 17–23.[CrossRef]
    [Google Scholar]
  8. Simpson, I. N., Gisby, J., Hemingway, C. P., Durodie, J. & Macpherson, I. ( 1995;). Evaluation of mupirocin E-test for determination of isolate susceptibility: comparison with standard agar dilution techniques. J Clin Microbiol 33, 2254–2259.
    [Google Scholar]
  9. Valdez, Y., Velapatiño, B., Gilman, R. H., Gutierrez, V. & León, C. ( 1998;). Antimicrobial susceptibility of Helicobacter pylori determined by the E test using tetrazolium egg yolk agar. J Clin Microbiol 36, 2784–2785.
    [Google Scholar]
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