1887

Abstract

Canine leptospirosis, which is caused by infection with pathogenic species, occurs worldwide, but information regarding the causative serotypes and genotypes and their effects on virulence in dogs remains limited. Monitoring acute leptospirosis in dogs as sentinels can also aid in estimating the risk of human leptospirosis, particularly when the disease is rare, as it currently is in Japan. Among 283 clinically suspected cases of leptospirosis diagnosed from August 2007 to March 2011 in Japan, 83 cases were laboratory diagnosed as leptospirosis by blood culture, a rise in antibody titres in paired sera using a microscopic agglutination test (MAT) and/or DNA detection using -nested PCR. The infected dogs comprised hunting dogs (31 dogs) and companion animals (50 dogs) and two unknown; 63.4 % of the infected dogs were males. The mortality rate was 53.2 %. A rise of at least fourfold in MAT titre was detected in 30 dogs whose paired serum samples were obtained, and the predominant reactive serogroup was Hebdomadis (53.3 %), followed by Australis (16.7 %) and Autumnalis (16.7 %). was isolated from 45 dogs of the following serogroups: Australis (16), Autumnalis (six), Canicola (one), Hebdomadis (21) and Icterohaemorrhagiae (one). All of these serogroups caused lethal infections (57.1–100 %). Genetic heterogeneity was demonstrated in serogroups Australis, Autumnalis and Hebdomadis by multilocus sequence typing (MLST) and/or RFLP analysis based on PFGE. In serogroup Hebdomadis, each genotype determined by MLST had a unique mortality rate in the infected dogs. Although classic canine leptospirosis is associated with serovars Canicola and Icterohaemorrhagiae, serogroup Hebdomadis has become the predominant serogroup causing high mortality in Japan. This study suggests that the virulence of members of serogroup Hebdomadis in dogs may be associated with the genotypes in this serogroup.

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2013-04-01
2019-12-07
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References

  1. Ahmed A., Thaipadungpanit J., Boonsilp S., Wuthiekanun V., Nalam K., Spratt B. G., Aanensen D. M., Smythe L. D., Ahmed N.. & other authors ( 2011;). Comparison of two multilocus sequence based genotyping schemes for Leptospira species. . PLoS Negl Trop Dis 5:, e1374. [CrossRef][PubMed]
    [Google Scholar]
  2. Bharti A. R., Nally J. E., Ricaldi J. N., Matthias M. A., Diaz M. M., Lovett M. A., Levett P. N., Gilman R. H., Willig M. R.. & other authors ( 2003;). Leptospirosis: a zoonotic disease of global importance. . Lancet Infect Dis 3:, 757–771. [CrossRef][PubMed]
    [Google Scholar]
  3. Ellis W. A.. ( 2010;). Control of canine leptospirosis in Europe: time for a change?. Vet Rec 167:, 602–605. [CrossRef][PubMed]
    [Google Scholar]
  4. Faine S., Adler B., Bolin C., Perolat P.. ( 1999;). Leptospira and Leptospirosis, , 2nd edn.. Melbourne:: MediSci;.
    [Google Scholar]
  5. Gautam R., Wu C. C., Guptill L. F., Potter A., Moore G. E., Moore G. E.. ( 2010;). Detection of antibodies against Leptospira serovars via microscopic agglutination tests in dogs in the United States, 2000–2007. . J Am Vet Med Assoc 237:, 293–298.[PubMed]
    [Google Scholar]
  6. Goldstein R. E.. ( 2010;). Canine leptospirosis. . Vet Clin North Am Small Anim Pract 40:, 1091–1101. [CrossRef][PubMed]
    [Google Scholar]
  7. Goldstein R. E., Lin R. C., Langston C. E., Scrivani P. V., Erb H. N., Barr S. C.. ( 2006;). Influence of infecting serogroup on clinical features of leptospirosis in dogs. . J Vet Intern Med 20:, 489–494. [CrossRef][PubMed]
    [Google Scholar]
  8. Gouveia E. L., Metcalfe J., de Carvalho A. L. F., Aires T. S. F., Villasboas-Bisneto J. C., Queirroz A., Santos A. C., Salgado K., Reis M. G., Ko A. I.. ( 2008;). Leptospirosis-associated severe pulmonary hemorrhagic syndrome, Salvador, Brazil. . Emerg Infect Dis 14:, 505–508. [CrossRef][PubMed]
    [Google Scholar]
  9. Greenlee J. J., Bolin C. A., Alt D. P., Cheville N. F., Andreasen C. B.. ( 2004;). Clinical and pathologic comparison of acute leptospirosis in dogs caused by two strains of Leptospira kirschneri serovar grippotyphosa. . Am J Vet Res 65:, 1100–1107. [CrossRef][PubMed]
    [Google Scholar]
  10. Greenlee J. J., Alt D. P., Bolin C. A., Zuerner R. L., Andreasen C. B.. ( 2005;). Experimental canine leptospirosis caused by Leptospira interrogans serovars pomona and bratislava. . Am J Vet Res 66:, 1816–1822. [CrossRef][PubMed]
    [Google Scholar]
  11. Gulig P. A., de Crécy-Lagard V., Wright A. C., Walts B., Telonis-Scott M., McIntyre L. M.. ( 2010;). SOLiD sequencing of four Vibrio vulnificus genomes enables comparative genomic analysis and identification of candidate clade-specific virulence genes. . BMC Genomics 11:, 512. [CrossRef][PubMed]
    [Google Scholar]
  12. Iwamoto E., Wada Y., Fujisaki Y., Umeki S., Jones M. Y., Mizuno T., Itamoto K., Maeda K., Iwata H., Okuda M.. ( 2009;). Nationwide survey of Leptospira antibodies in dogs in Japan: results from microscopic agglutination test and enzyme-linked immunosorbent assay. . J Vet Med Sci 71:, 1191–1199. [CrossRef][PubMed]
    [Google Scholar]
  13. Koizumi N., Muto M., Yamamoto S., Baba Y., Kudo M., Tamae Y., Shimomura K., Takatori I., Iwakiri A.. & other authors ( 2008;). Investigation of reservoir animals of Leptospira in the northern part of Miyazaki Prefecture. . Jpn J Infect Dis 61:, 465–468.[PubMed]
    [Google Scholar]
  14. Koizumi N., Uchida M., Makino T., Taguri T., Kuroki T., Muto M., Kato Y., Watanabe H.. ( 2009;). Isolation and characterization of Leptospira spp. from raccoons in Japan. . J Vet Med Sci 71:, 425–429. [CrossRef][PubMed]
    [Google Scholar]
  15. Levett P. N.. ( 2001;). Leptospirosis. . Clin Microbiol Rev 14:, 296–326. [CrossRef][PubMed]
    [Google Scholar]
  16. Maiden M. C.. ( 2006;). Multilocus sequence typing of bacteria. . Annu Rev Microbiol 60:, 561–588. [CrossRef][PubMed]
    [Google Scholar]
  17. Martins G., Penna B., Lilenbaum W.. ( 2012;). The dog in the transmission of human leptospirosis under tropical conditions: victim or villain?. Epidemiol Infect 140:, 207–208, author reply 208–209. [CrossRef][PubMed]
    [Google Scholar]
  18. Nash J. H., Villegas A., Kropinski A. M., Aguilar-Valenzuela R., Konczy P., Mascarenhas M., Ziebell K., Torres A. G., Karmali M. A., Coombes B. K.. ( 2010;). Genome sequence of adherent-invasive Escherichia coli and comparative genomic analysis with other E. coli pathotypes. . BMC Genomics 11:, 667. [CrossRef][PubMed]
    [Google Scholar]
  19. Stokes J. E., Kaneene J. B., Schall W. D., Kruger J. M., Miller R., Kaiser L., Bolin C. A.. ( 2007;). Prevalence of serum antibodies against six Leptospira serovars in healthy dogs. . J Am Vet Med Assoc 230:, 1657–1664. [CrossRef][PubMed]
    [Google Scholar]
  20. Suepaul S. M., Carrington C. V. F., Campbell M., Borde G., Adesiyun A. A.. ( 2010;). Serovars of Leptospira isolated from dogs and rodents. . Epidemiol Infect 138:, 1059–1070. [CrossRef][PubMed]
    [Google Scholar]
  21. Sykes J. E., Hartmann K., Lunn K. F., Moore G. E., Stoddard R. A., Goldstein R. E.. ( 2011;). 2010 ACVIM small animal consensus statement on leptospirosis: diagnosis, epidemiology, treatment, and prevention. . J Vet Intern Med 25:, 1–13. [CrossRef][PubMed]
    [Google Scholar]
  22. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  23. Thaipadungpanit J., Wuthiekanun V., Chierakul W., Smythe L. D., Petkanchanapong W., Limpaiboon R., Apiwatanaporn A., Slack A. T., Suputtamongkol Y.. & other authors ( 2007;). A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand. . PLoS Negl Trop Dis 1:, e56. [CrossRef][PubMed]
    [Google Scholar]
  24. Yanagawa R.. ( 1992;). [ Animal leptospirosis. ]. Kagaku Ryoho no Ryoiki 8:, 673–678 (in Japanese).
    [Google Scholar]
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