1887

Abstract

A detection system for DNA in blood samples based on the PCR was developed and evaluated in A/J mice with experimentally induced pneumonia. Primers were designed to amplify a 106 bp DNA fragment of the 16S rRNA gene specific to species. The PCR system could detect clinically relevant species including , , , , , and . The sensitivity of the PCR system was 20 fg extracted DNA. In the mouse model, the blood PCR was compared with results obtained by PCR on bronchoalveolar lavage fluid (BALF) samples, cultures of blood and BALF and detection of urinary antigen. Blood PCR was positive until 8 days after infection, while BALF PCR became negative on day 4. These results indicate that PCR using blood samples may be a useful, convenient and non-invasive method for the diagnosis of pneumonia.

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2003-04-01
2024-03-29
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