1887

Abstract

The importance of hens eggs as a source of specific antibodies (IgY) is well recognized. The protective effect of IgY obtained from hens immunized with whole-cell lysate has been reported for the control of infection. However, IgY produced by whole-cell lysates presents the possibility of cross-reactivity with other bacteria, including the normal human flora, and this could decrease the efficiency of IgY. In the present study, the immunodominant proteins of with reactivity to -specific IgY (IgY-) were identified. IgY obtained from hens immunized with various fractions of proteins was isolated and purified, titres of IgY- against were determined and cross-reactivity between IgY- and normal human bacteria was examined by Western blot analysis. Finally, immunodominant proteins were identified by LC/MS analysis. IgY obtained 2 months after immunization with whole-cell lysate showed the highest antibody titre. Five immunodominant proteins were identified that were strongly reactive to IgY-: urease β-subunit (62 kDa), heat-shock protein 60 (60 kDa), urease α-subunit (26 kDa), probable peroxiredoxin (22 kDa) and probable thiol peroxidase (18 kDa). Immunization of hens with the immunodominant proteins identified would produce a more specific IgY against .

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2003-03-01
2020-01-17
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References

  1. Akita, E. M. & Nakai, S. ( 1992;). Immunoglobulin from egg yolk: isolation and purification. J Food Sci 57, 629–634.[CrossRef]
    [Google Scholar]
  2. Akita, E. M. & Nakai, S. ( 1993;). Comparison of four purification methods for the production of immunoglobulin from eggs laid by hens immunized with an enterotoxigenic E. coli strain. J Immunol Methods 160, 207–214.[CrossRef]
    [Google Scholar]
  3. Anonymous ( 1997;). Current European concepts in the management of Helicobacter pylori infection. The Maastricht Consensus Report. European Helicobacter pylori Study Group. Gut 41, 8–13.[CrossRef]
    [Google Scholar]
  4. Censini, S., Lange, C., Xiang, Z., Crabtree, J. E., Ghiara, P., Borodovsky, M., Rappuoli, R. & Covacci, A. ( 1996;). cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc Natl Acad Sci U S A 93, 14648–14653.[CrossRef]
    [Google Scholar]
  5. Chakravarti, D. N., Fiske, M. J., Fletcher, L. D. & Zagursky, R. J. ( 2000;). Application of genomics and proteomics for identification of bacterial gene products as potential vaccine candidates. Vaccine 19, 601–612.[CrossRef]
    [Google Scholar]
  6. Cover, T. L. & Blaser, M. J. ( 1992;). Purification and characterization of the vacuolating toxin from Helicobacter pylori. J Biol Chem 267, 10570–10575.
    [Google Scholar]
  7. Goddard, A. F. & Logan, R. P. (1996). Antimicrobial resistance and Helicobacter pylori. J Antimicrob Chemother 37, 639–643.
  8. Graham, D. Y., Go, M. F. & Evans, D. J., Jr ( 1992;). Urease, gastric ammonium/ammonia, and Helicobacter pylori – the past, the present, and recommendations for future research. Aliment Pharmacol Ther 6, 659–669.
    [Google Scholar]
  9. Hatta, H., Tsuda, K., Akachi, S., Kim, M., Yamamoto, T. & Ebina, T. ( 1993;). Oral passive immunization effect of anti-human rotavirus IgY and its behavior against proteolytic enzymes. Biosci Biotechnol Biochem 57, 1077–1081.[CrossRef]
    [Google Scholar]
  10. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans ( 1994;). Infection with Helicobacter pylori. IARC Monogr Eval Carcinog Risks Hum 61, 177–240.
    [Google Scholar]
  11. Keller, W. C. & Michetti, P. ( 2001;). Vaccination against Helicobacter pylori –an old companion of man. Expert Opin Biol Ther 1, 795–802.[CrossRef]
    [Google Scholar]
  12. Kim, B. J., Kang, B. H., Kim, T. Y., Kim, T. H. & Kim, K. W. ( 1997;). Production and characterization of IgY specific to Helicobacter pylori. Kor J Appl Microbiol Biotechnol 25, 612–616.
    [Google Scholar]
  13. Kim, B. W., Choi, M. G., Choi, H. & 8 other authors ( 1999;). Pooled analysis of antibiotic therapy for Helicobacter pylori eradication in Korea. Kor J Gastroenterol 34, 42–49.
    [Google Scholar]
  14. Korean H. pylori Study Group ( 1998;). Diagnosis and treatment of Helicobacter pylori infection in Korea. Kor J Gastroenterol 32, 275–289.
    [Google Scholar]
  15. Laemmli, U. K. ( 1970;). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  16. Mabe, K., Yamada, M., Oguni, I. & Takahashi, T. ( 1999;). In vitro and in vivo activities of tea catechins against Helicobacter pylori. Antimicrob Agents Chemother 43, 1788–1791.
    [Google Scholar]
  17. Michetti, P., Corthésy-Theulaz, I., Davin, C. & 7 other authors ( 1994;). Immunization of BALB/c mice against Helicobacter felis infection with Helicobacter pylori urease. Gastroenterology 107, 1002–1011.
    [Google Scholar]
  18. Michetti, P., Kreiss, C., Kotloff, K. L. & 13 other authors ( 1999;). Oral immunization with urease and Escherichia coli heat-labile enterotoxin is safe and immunogenic in Helicobacter pylori-infected adults. Gastroenterology 116, 804–812.[CrossRef]
    [Google Scholar]
  19. Mizushima, T., Sugiyama, T., Komatsu, Y., Ishizuka, J., Kato, M. & Asaka, M. ( 2001;). Clinical relevance of the babA2 genotype of Helicobacter pylori in Japanese clinical isolates. J Clin Microbiol 39, 2463–2465.[CrossRef]
    [Google Scholar]
  20. Mysore, J. V., Wigginton, T., Simon, P. M., Zopf, D., Heman-Ackah, L. M. & Dubois, A. ( 1999;). Treatment of Helicobacter pylori infection in rhesus monkeys using a novel antiadhesion compound. Gastroenterology 117, 1316–1325.[CrossRef]
    [Google Scholar]
  21. Nam, S. W., Roe, I. H., Kim, S. B. & 7 other authors ( 2000;). Detection of clarithromycin-resistant Helicobacter pylori by polymerase chain reaction. Kor J Gastroenterol 36, 450–456.
    [Google Scholar]
  22. Porwollik, S., Noonan, B. & O'Toole, P. W. ( 1999;). Molecular characterization of a flagellar export locus of Helicobacter pylori. Infect Immun 67, 2060–2070.
    [Google Scholar]
  23. Roe, I. H., Nam, S. U., Yang, M. R., Myung, N. H., Kim, J. T. & Shin, J. H. ( 2002;). The promising effect of egg yolk antibody IgY on the treatment of Helicobacter pylori-associated gastric diseases. Kor J Gastroenterol 39, 260–268.
    [Google Scholar]
  24. Satoh, K., Kimura, K., Taniguchi, Y. & 12 other authors ( 1996;). Distribution of inflammation and atrophy in the stomach of Helicobacter pylori-positive and -negative patients with chronic gastritis. Am J Gastroenterol 91, 963–969.
    [Google Scholar]
  25. Sharma, S. A., Miller, G. G., Peek, R. A., Jr, Pérez-Pérez, G. & Blaser, M. J. ( 1997;). T-cell, antibody, and cytokine responses to homologs of the 60-kilodalton heat shock protein in Helicobacter pylori infection. Clin Diagn Lab Immunol 4, 440–446.
    [Google Scholar]
  26. Shon, D. H., Roe, J. H., Kim, Y. B., Han, C. K., Sung, K. S. & Lee, N. H. ( 1998;). Properties of anti-S. mutans IgY separated from egg yolk. Kor J Food Sci Technol 30, 1029–1034.
    [Google Scholar]
  27. Simon, P. M., Goode, P. L., Mobasseri, A. & Zopf, D. ( 1997;). Inhibition of Helicobacter pylori binding to gastrointestinal epithelial cells by sialic acid-containing oligosaccharides. Infect Immun 65, 750–757.
    [Google Scholar]
  28. Smith, D. J., King, W. F. & Godiska, R. ( 2001;). Passive transfer of immunoglobulin Y antibody to Streptococcus mutans glucan binding protein B can confer protection against experimental dental caries. Infect Immun 69, 3135–3142.[CrossRef]
    [Google Scholar]
  29. Sunwoo, H. H., Nakano, T., Dixon, W. T. & Sim, J. S. (1996). Immune responses in chickens against lipopolysaccharide of Escherichia coli and Salmonella typhimurium. Poult Sci 75, 342–345.
  30. Yamaguchi, H., Osaki, T., Kai, M., Taguchi, H. & Kamiya, S. ( 2000;). Immune response against a cross-reactive epitope on the heat shock protein 60 homologue of Helicobacter pylori. Infect Immun 68, 3448–3454.[CrossRef]
    [Google Scholar]
  31. Yokoyama, H., Peralta, C. R., Diaz, R., Sendo, S., Ikemori, Y. & Kodama, Y. ( 1992;). Passive protective effect of chicken egg yolk immunoglobulins against experimental enterotoxigenic Escherichia coli infection in neonatal piglets. Infect Immun 60, 998–1007.
    [Google Scholar]
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