1887

Abstract

The importance of hens eggs as a source of specific antibodies (IgY) is well recognized. The protective effect of IgY obtained from hens immunized with whole-cell lysate has been reported for the control of infection. However, IgY produced by whole-cell lysates presents the possibility of cross-reactivity with other bacteria, including the normal human flora, and this could decrease the efficiency of IgY. In the present study, the immunodominant proteins of with reactivity to -specific IgY (IgY-) were identified. IgY obtained from hens immunized with various fractions of proteins was isolated and purified, titres of IgY- against were determined and cross-reactivity between IgY- and normal human bacteria was examined by Western blot analysis. Finally, immunodominant proteins were identified by LC/MS analysis. IgY obtained 2 months after immunization with whole-cell lysate showed the highest antibody titre. Five immunodominant proteins were identified that were strongly reactive to IgY-: urease β-subunit (62 kDa), heat-shock protein 60 (60 kDa), urease α-subunit (26 kDa), probable peroxiredoxin (22 kDa) and probable thiol peroxidase (18 kDa). Immunization of hens with the immunodominant proteins identified would produce a more specific IgY against .

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2003-03-01
2021-07-24
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