1887

Abstract

Attaching and effacing (AEEC) have been implicated in diarrhoea in humans in several countries. A total of 919 strains, isolated from 125 children with diarrhoea and 98 without diarrhoea, was investigated by PCR for the presence of the EAF, , and genes. Thirty-four of these isolates were found to carry the gene; they were isolated from 27 (79.4 %) children with diarrhoea and seven (20.6 %) controls, in the city of Londrina, Brazil. These strains were investigated for their genotypic and phenotypic characteristics. Different genetic profiles were observed; strains containing the gene alone were most common (47.1 %). The characteristic genetic profile of typical enteropathogenic (EPEC), , and EAF, was only found in isolates from children with diarrhoea. The gene was not detected in any of the 34 strains studied. Ten (29.4 %) strains were negative in the fluorescent actin-staining test. Localized adhesion (LA) was the most common pattern of adhesion (44.1 %), followed by the aggregative adhesion (AA) (23.5 %) and localized adhesion-like (LAL) (14.7 %) patterns. The results showed a strong association between strains presenting the LA pattern and diarrhoea. Forty-seven per cent of the strains studied belonged to classical O-serogroups of EPEC. The most common serotype found was O119 : H6; these isolates all showed the LA pattern, were positive for fluorescent actin-staining and were associated with diarrhoea. Intimin β was detected in seven strains, four of which belonged to serotype O119 : H6 and three to serotype ONT : H7; all were associated with diarrhoea. On the other hand, intimin ϵ was detected in two strains of serotype O111 : H38 and one of serotype ONT : H19, isolated from children without diarrhoea. To our knowledge, this is the first report of the occurrence of intimin ϵ in strains of isolated from humans in Brazil.

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2003-06-01
2020-10-29
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