1887

Abstract

Attaching and effacing (AEEC) have been implicated in diarrhoea in humans in several countries. A total of 919 strains, isolated from 125 children with diarrhoea and 98 without diarrhoea, was investigated by PCR for the presence of the EAF, , and genes. Thirty-four of these isolates were found to carry the gene; they were isolated from 27 (79.4 %) children with diarrhoea and seven (20.6 %) controls, in the city of Londrina, Brazil. These strains were investigated for their genotypic and phenotypic characteristics. Different genetic profiles were observed; strains containing the gene alone were most common (47.1 %). The characteristic genetic profile of typical enteropathogenic (EPEC), , and EAF, was only found in isolates from children with diarrhoea. The gene was not detected in any of the 34 strains studied. Ten (29.4 %) strains were negative in the fluorescent actin-staining test. Localized adhesion (LA) was the most common pattern of adhesion (44.1 %), followed by the aggregative adhesion (AA) (23.5 %) and localized adhesion-like (LAL) (14.7 %) patterns. The results showed a strong association between strains presenting the LA pattern and diarrhoea. Forty-seven per cent of the strains studied belonged to classical O-serogroups of EPEC. The most common serotype found was O119 : H6; these isolates all showed the LA pattern, were positive for fluorescent actin-staining and were associated with diarrhoea. Intimin β was detected in seven strains, four of which belonged to serotype O119 : H6 and three to serotype ONT : H7; all were associated with diarrhoea. On the other hand, intimin ϵ was detected in two strains of serotype O111 : H38 and one of serotype ONT : H19, isolated from children without diarrhoea. To our knowledge, this is the first report of the occurrence of intimin ϵ in strains of isolated from humans in Brazil.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.04977-0
2003-06-01
2019-11-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/52/6/JM520609.html?itemId=/content/journal/jmm/10.1099/jmm.0.04977-0&mimeType=html&fmt=ahah

References

  1. Adu-Bobie, J., Frankel, G., Bain, C., Goncalves, A. G., Trabulsi, L. R., Douce, G., Knutton, S. & Dougan, G. ( 1998;). Detection of intimins α, β, γ and δ, four intimin derivatives expressed by attaching and effacing microbial pathogens. J Clin Microbiol 36, 662–668.
    [Google Scholar]
  2. Agin, T. S. & Wolf, M. K. ( 1997;). Identification of a family of intimins common to Escherichia coli causing attaching–effacing lesions in rabbits, humans, and swine. Infect Immun 65, 320–326.
    [Google Scholar]
  3. Aidar, L., Penteado, A. S., Trabulsi, L. R., Blanco, J. E., Blanco, M., Blanco, J. & Pestana de Castro, A. F. ( 2000;). Subtypes of intimin among non-toxigenic Escherichia coli from diarrheic calves in Brazil. Can J Vet Res 64, 15–20.
    [Google Scholar]
  4. Baldini, M. M., Kaper, J. B., Levine, M. M., Candy, D. C. A. & Moon, H. W. ( 1983;). Plasmid-mediated adhesion in enteropathogenic Escherichia coli. J Pediatr Gastroenterol Nutr 2, 534–538.[CrossRef]
    [Google Scholar]
  5. Baudry, B., Savarino, S. J., Vial, P., Kaper, J. B. & Levine, M. M. ( 1990;). A sensitive and specific DNA probe to identify enteroaggregative Escherichia coli, a recently discovered diarrheal pathogen. J Infect Dis 161, 1249–1251.[CrossRef]
    [Google Scholar]
  6. Beebakhee, G., Louie, M., De Azavedo, J. & Brunton, J. ( 1992;). Cloning and nucleotide sequence of the eae gene homologue from enterohemorrhagic Escherichia coli serotype O157 : H7. FEMS Microbiol Lett 91, 63–68.[CrossRef]
    [Google Scholar]
  7. Beutin, L. ( 1991;). The different hemolysins of Escherichia coli. Med Microbiol Immunol 180, 167–182.
    [Google Scholar]
  8. Beutin, L., Zimmermann, S. & Gleier, K. ( 1998;). Human infections with Shiga toxin-producing Escherichia coli other than serogroup O157 in Germany. Emerg Infect Dis 4, 635–639.[CrossRef]
    [Google Scholar]
  9. Cantey, J. R. & Moseley, S. L. ( 1991;). HeLa cell adherence, actin aggregation, and invasion by nonenteropathogenic Escherichia coli possessing the eae gene. Infect Immun 59, 3924–3929.
    [Google Scholar]
  10. Cravioto, A., Gross, R. J., Scotland, S. M. & Rowe, B. ( 1979;). An adhesive factor found in strains of Escherichia coli belonging to the traditional infantile enteropathogenic serotypes. Curr Microbiol 3, 95–99.[CrossRef]
    [Google Scholar]
  11. Edwards, P. R. & Ewing, W. H. ( 1972;). Identification of Enterobacteriaceae, 3rd edn. Minneapolis, MN: Burgess.
  12. Franke, J., Franke, S., Schmidt, H., Schwarzkopf, A., Wieler, L. H., Baljer, G., Beutin, L. & Karch, H. ( 1994;). Nucleotide sequence analysis of enteropathogenic Escherichia coli (EPEC) adherence factor probe and development of PCR for rapid detection of EPEC harboring virulence plasmids. J Clin Microbiol 32, 2460–2463.
    [Google Scholar]
  13. Frankel, G., Candy, D. C., Everest, P. & Dougan, G. ( 1994;). Characterization of the C-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic Escherichia coli, Citrobacter freundii, and Hafnia alvei. Infect Immun 62, 1835–1842.
    [Google Scholar]
  14. Frankel, G., Candy, D. C. A., Fabiani, E., Adu-Bobie, J., Gil, S., Novakova, M., Phillips, A. D. & Dougan, G. ( 1995;). Molecular characterization of a carboxy-terminal eukaryotic-cell-binding domain of intimin from enteropathogenic Escherichia coli. Infect Immun 63, 4323–4328.
    [Google Scholar]
  15. Giraldi, R., Guth, B. E. C. & Trabulsi, L. R. ( 1990;). Production of Shiga-like toxin among Escherichia coli strains and other bacteria isolated from diarrhea in São Paulo, Brazil. J Clin Microbiol 28, 1460–1462.
    [Google Scholar]
  16. Girón, J. A., Ho, A. S. Y. & Schoolnik, G. K. ( 1991;). An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science 254, 710–713.[CrossRef]
    [Google Scholar]
  17. Gomes, T. A. T., Rassi, V., MacDonald, K. L. & 8 other authors ( 1991;). Enteropathogens associated with acute diarrheal disease in urban infants in São Paulo, Brazil. J Infect Dis 164, 331–337.[CrossRef]
    [Google Scholar]
  18. Gunzberg, S. T., Tornieporth, N. G. & Riley, L. W. ( 1995;). Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene. J Clin Microbiol 33, 1375–1377.
    [Google Scholar]
  19. Jerse, A. E., Yu, J., Tall, B. D. & Kaper, J. B. ( 1990;). A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci U S A 87, 7839–7843.[CrossRef]
    [Google Scholar]
  20. Kaper, J. B. ( 1996;). Defining EPEC. Rev Microbiol 27 (Suppl. 1), 130–133.
    [Google Scholar]
  21. Kenny, B., DeVinney, R., Stein, M., Reinscheid, D. J., Frey, E. A. & Finlay, B. B. ( 1997;). Enteropathogenic E.coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell 91, 511–520.[CrossRef]
    [Google Scholar]
  22. Knutton, S., Baldwin, T., Williams, P. H. & McNeish, A. S. ( 1989;). Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun 57, 1290–1298.
    [Google Scholar]
  23. Lin, Z., Kurazono, H., Yamasaki, S. & Takeda, Y. ( 1993;). Detection of various variant verotoxin genes in Escherichia coli by polymerase chain reaction. Microbiol Immunol 37, 543–548.[CrossRef]
    [Google Scholar]
  24. Louie, M., de Azavedo, J. C., Handelsman, M. Y. C., Clark, C. G., Ally, B., Dytoc, M., Sherman, P. & Brunton, J. ( 1993;). Expression and characterization of the eaeA gene product of Escherichia coli serotype O157 : H7. Infect Immun 61, 4085–4092.
    [Google Scholar]
  25. McDaniel, T. K., Jarvis, K. G., Donnenberg, M. S. & Kaper, J. B. ( 1995;). A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc Natl Acad Sci USA 92, 1664–1668.[CrossRef]
    [Google Scholar]
  26. Nataro, J. P. & Kaper, J. B. ( 1998;). Diarrheagenic Escherichia coli. Clin Microbiol Rev 11, 142–201.
    [Google Scholar]
  27. Oswald, E., Schmidt, H., Morabito, S., Karch, H., Marchès, O. & Caprioli, A. ( 2000;). Typing of intimin genes in human and animal enterohemorrhagic and enteropathogenic Escherichia coli: characterization of a new intimin variant. Infect Immun 68, 64–71.[CrossRef]
    [Google Scholar]
  28. Pelayo, J. S., Scaletsky, I. C. A., Pedroso, M. Z., Sperandio, V., Girón, J. A., Frankel, G. & Trabulsi, L. R. ( 1999;). Virulence properties of atypical EPEC strains. J Med Microbiol 48, 41–49.[CrossRef]
    [Google Scholar]
  29. Saridakis, H. O. ( 1994;). Non production of Shiga-like toxins by Escherichia coli serogroup O26. Rev Microbiol 25, 154–155.
    [Google Scholar]
  30. Scaletsky, I. C. A., Silva, M. L. M. & Trabulsi, L. R. ( 1984;). Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells. Infect Immun 45, 534–536.
    [Google Scholar]
  31. Scaletsky, I. C. A., Pelayo, J. S., Giraldi, R., Rodrigues, J., Pedroso, M. Z. & Trabulsi, L. R. ( 1996;). EPEC adherence to HEp-2 cells. Rev Microbiol 27 (Suppl. 1), 58–62.
    [Google Scholar]
  32. Scaletsky, I. C. A., Pedroso, M. Z., Oliva, C. A. G., Carvalho, R. B., Morais, M. B. & Fagundes-Neto, U. ( 1999;). A localized adherence-like pattern as a second pattern of adherence of classic enteropathogenic Escherichia coli to HEp-2 cells that is associated with infantile diarrhea. Infect Immun 67, 3410–3415.
    [Google Scholar]
  33. Vieira, M. A. M., Andrade, J. R. C., Trabulsi, L. R., Rosa, A. C., Dias, A. M. G., Ramos, S. R. T. S., Frankel, G. & Gomes, T. A. T. ( 2001;). Phenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E.coli (EPEC) serogroups that carry eae and lack the EPEC adherence factor and Shiga toxin DNA probe sequences. J Infect Dis 183, 762–772.[CrossRef]
    [Google Scholar]
  34. Yu, J. & Kaper, J. B. ( 1992;). Cloning and characterization of the eae gene of enterohaemorragic Escherichia coli O157 : H7. Mol Microbiol 6, 411–417.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.04977-0
Loading
/content/journal/jmm/10.1099/jmm.0.04977-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error