1887

Journal of Medical Microbiology logo

Volume 52, Issue 8

Genetic relatedness of strains isolated from women with vaginal candidiasis in Malaysia Free

Abstract

The aims of this study were to compare the genetic relatedness of: (i) sequential and single isolates of strains from women with recurrent vaginal candidiasis (RVC); and (ii) strains from women who had only one episode of infection within a 1-year period. In total, 87 isolates from 71 patients were cultured, speciated and genotyped by random amplification of polymorphic DNA (RAPD) analysis. Patients were categorized into three groups, namely those with: (i) a history of RVC from whom two or more yeast isolates were obtained (group A); (ii) a history of RVC from whom only a single isolate was obtained (group B); and (iii) a single episode of vaginal candidiasis within a 1-year period (group C). Six yeast species were detected: , , , , and . Interestingly, the prevalence of non- species was higher in group A patients (50 %) than in patients in groups B (36 %) or C (18.9 %). Eighty RAPD profiles were observed, with a total of 61 polymorphic PCR fragments of distinct sizes. Clustering analysis showed that, overall, the majority of patients in group A had recurrent infections caused by highly similar, but not identical, sequential strains [mean pairwise similarity coefficient ( ) = 0.721 ± 0.308]. The range of mean values for intergroup comparisons for isolates alone was 0.50–0.56, suggesting that there was no significant relatedness between strains from different groups. Genetic similarity of isolates from patients in group A was lower than that of isolates from patients in group C (mean = 0.532 ± 0.249 and 0.636 ± 0.206, respectively); this difference was statistically significant ( = 0.036). These results demonstrate that the cause of recurrent infections varies among individuals and ranges between strain maintenance, strain microevolution and strain replacement; the major scenario is strain maintenance with microevolution. They also show that strains that cause recurrent infections are less similar to each other than strains that cause one-off infections, suggesting that the former may represent more virulent subtypes.

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.04973-0
2003-08-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/52/8/JM520809.html?itemId=/content/journal/jmm/10.1099/jmm.0.04973-0&mimeType=html&fmt=ahah

References

  1. Armstrong J, Gibbs A, Peakall R, Weiller G. 1994 The RAPDistance package (available at http://biology.anu.edu.au/ research-groups/gig/bi/molecular/software/rapid.htm
  2. Biswas S. K, Yokoyama K, Wang L, Nishimura K, Miyaji M. 2001; Typing of Candida albicans isolates by sequence analysis of the cytochrome b gene and differentiation from Candida stellatoidea . J Clin Microbiol 39:1600–1603 [CrossRef]
     [Google Scholar]
  3. Dice L. R. 1945; Measures of the amount of ecologic associations between species. J Ecol 26:197–302
     [Google Scholar]
  4. Faro S. 1996; Vaginitis: diagnosis and management. Int J Fertil Menopausal Stud 41:115–123
     [Google Scholar]
  5. Fidel P. L Jr, Sobel J. D. 1996; Immunopathogenesis of recurrent vulvovaginal candidiasis. Clin Microbiol Rev 9:335–348
     [Google Scholar]
  6. Fidel P. L Jr, Vazquez J. A, Sobel J. D. 1999; Candida glabrata : review of epidemiology, pathogenesis and clinical disease with comparison to C.albicans . Clin Microbiol Rev 12:80–96
     [Google Scholar]
  7. Kumar S, Tamura K, Jakobsen I. B, Nei M. 2001; mega2: molecular evolutionary genetic analysis software. Bioinformatics 17:1244–1245 [CrossRef]
     [Google Scholar]
  8. Lanchares J. L, Hernandez M. L. 2000; Recurrent vaginal candidiasis changes in etiopathogenical patterns. Int J Gynaecol Obstet 71:Suppl. 1S29–S35 [CrossRef]
     [Google Scholar]
  9. Lockhart S. R, Reed B. D, Pierson C. L, Soll D. R. 1996; Most frequent scenario for recurrent Candida vaginitis is strain maintenance with ‘‘substrain shuffling’': demonstration by sequential DNA fingerprinting with probes Ca3, C1, and CARE2. J Clin Microbiol 34:767–777
     [Google Scholar]
  10. Marco F, Lockhart S. R, Pfaller M. A. 11 other authors 1999; Elucidating the origins of nosocomial infections with Candida albicans by DNA fingerprinting with the complex probe Ca3.The Nemis Study Group. J Clin Microbiol 37:2817–2828
     [Google Scholar]
  11. McCullough M. J, Clemons K. V, Stevens D. A. 1999; Molecular and phenotypic characterization of genotypic Candida albicans subgroups and comparison with Candida dubliniensis and Candida stellatoidea . J Clin Microbiol 37:417–421
     [Google Scholar]
  12. Mercure S, Poirier S, Lemay G, Auger P, Montplaisir S, de Repentigny L. 1993; Application of biotyping and DNA typing of Candida albicans to the epidemiology of recurrent vulvovaginal candidiasis. J Infect Dis 168:502–507 [CrossRef]
     [Google Scholar]
  13. Metzgar D, van Belkum A, Field D, Haubrich R, Wills C. 1998; Random amplification of polymorphic DNA and microsatellite genotyping of pre- and posttreatment isolates of Candida spp.from human immunodeficiency virus-infected patients on different fluconazole regimens. J Clin Microbiol 36:2308–2313
     [Google Scholar]
  14. Ng K. P, Madasamy M, Saw T. L, Baki A, He J, Soo-Hoo T. S. 1999; Candida biotypes isolated from clinical specimens in Malaysia. Mycopathologia 144:135–140
     [Google Scholar]
  15. Nyirjesy P, Seeney S. M, Grody M. H, Jordan C. A, Buckley H. R. 1995; Chronic fungal vaginitis: the value of cultures. Am J Obstet Gynecol 173:820–823 [CrossRef]
     [Google Scholar]
  16. Ringdahl E. N. 2000; Treatment of recurrent vulvovaginal candidiasis. Am Fam Physician 61:3306–3312, 3317. 3317
     [Google Scholar]
  17. Rippon J. W. 1974 Medical Mycology: the Pathogenic Fungi and the Pathogenic Actinomycetes pp 33–70 Philadelphia: Saunders;
     [Google Scholar]
  18. Samaranayake Y. H, Samaranayake L. P. 1994; Candida krusei : biology, epidemiology, pathogenicity and clinical manifestations of an emerging pathogen. J Med Microbiol 41:295–310 [CrossRef]
     [Google Scholar]
  19. Sobel J. D. 1992; Pathogenesis and treatment of recurrent vulvovaginal candidiasis. Clin Infect Dis 14:Suppl. 1S148–S153 [CrossRef]
     [Google Scholar]
  20. Sobel J. D. 1993; Candidal vulvovaginitis. Clin Obstet Gynecol 36:153–165 [CrossRef]
     [Google Scholar]
  21. Vazquez J. A, Sobel J. D, Demitriou R, Vaishampayan J, Lynch M, Zervos M. J. 1994; Karyotyping of Candida albicans isolates obtained longitudinally in women with recurrent vulvovaginal candidiasis. J Infect Dis 170:1566–1569 [CrossRef]
     [Google Scholar]
  22. Xu J, Boyd C. M, Livingston E, Meyer W, Madden J. F, Mitchell T. G. 1999; Species and genotypic diversities and similarities of pathogenic yeasts colonizing women. J Clin Microbiol 37:3835–3843
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.04973-0
Loading
Genetic relatedness of Candida strains isolated from women with vaginal candidiasis in Malaysia
J. Med. Microbiol. 52, 657 (2003); https://doi.org/10.1099/jmm.0.04973-0
/content/journal/jmm/10.1099/jmm.0.04973-0
/content/journal/jmm/10.1099/jmm.0.04973-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error