1887

Abstract

The nucleotide sequence of an ORF () within the type IV pilus gene cluster of O34 strain NAGV14 was determined, thereby completing the sequence analysis of the structural operon. The gene showed homology to the gene of the mannose-sensitive haemagglutinin pilus gene cluster. The was 651 bp larger than , and the G+C content of the extra 651 bp portion (35.6 mol%) was lower than that of the overall gene (42.5 mol%). Except for the first 270 aa residues, the deduced amino acid sequence of VcfQ showed high homology to the MshQ protein. There was immunological cross-reaction between VcfQ and MshQ by Western blotting. Cell fractionation studies showed that VcfQ is located in both the inner and the outer membranes. Mutational analysis showed that -deficient mutant expressed detectable levels of major pilin (VcfA), but failed to assemble them into pili, indicating that VcfQ is essential for pilus assembly. Colony-blotting analyses showed that the N-terminal region of is variable in strains.

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2003-04-01
2019-10-22
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